Development, Vol 104, Issue 1 165-173, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Complete sequence and in vitro expression of a tissue-specific phosphatidylinositol-linked N-CAM isoform from skeletal muscle

CH Barton, G Dickson, HJ Gower, LH Rowett, W Putt, V Elsom, SE Moore, C Goridis and FS Walsh
Institute of Neurology, London, UK.

Neural cell adhesion molecules (N-CAMs) are a family of cell surface sialoglycoproteins encoded by a single copy gene. A full-length cDNA clone that encodes a nontransmembrane phosphatidylinositol (PI) linked N-CAM of Mr 125 x 10(3) has been isolated from a human skeletal muscle cDNA library. The deduced protein sequence encodes a polypeptide of 761 amino acids and is highly homologous to the N-CAM isoform in brain of Mr 120 x 10(3). The size difference between the 125 x 10(3). The size difference between the 125 x 10(3) Mr skeletal muscle form and the 120 x 10(3) Mr N-CAM form from brain is accounted for by the insertion of a block of 37 amino acids called MSD1, in the extracellular domain of the muscle form. Transient expression of the human cDNA in COS cells results in cell surface N-CAM expression via a putative covalent attachment to PI-containing phospholipid. Linked in vitro transcription and translation experiments followed by immunoprecipitation with anti-N-CAM antibodies demonstrate that the full-length clone of 761 amino acid coding potential produces a core polypeptide of Mr 110 x 10(3) which is processed by microsomal membranes to yield a 122 x 10(3) Mr species. Taken together, these results demonstrate that the cloned cDNA sequence encodes a lipid-linked, PI-specific phospholipase C releasable surface isoform of N-CAM with core glycopeptide molecular weight corresponding to the authentic muscle 125 x 10(3) Mr N-CAM isoform. This is the first direct correlation of cDNA and deduced protein sequence with a known PI-linked N-CAM isoform from skeletal muscle.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. B. Nicholson and C. P. Stanners Specific inhibition of GPI-anchored protein function by homing and self-association of specific GPI anchors J. Cell Biol., November 20, 2006; 175(4): 647 - 659. [Abstract] [Full Text] [PDF]

				E. Sanchez-Heras, F. V. Howell, G. Williams, and P. Doherty The Fibroblast Growth Factor Receptor Acid Box Is Essential for Interactions with N-Cadherin and All of the Major Isoforms of Neural Cell Adhesion Molecule J. Biol. Chem., November 17, 2006; 281(46): 35208 - 35216. [Abstract] [Full Text] [PDF]

				R. A. Screaton, L. DeMarte, P. Draber, and C. P. Stanners The Specificity for the Differentiation Blocking Activity of Carcinoembryonic Antigen Resides in Its Glycophosphatidyl-Inositol Anchor J. Cell Biol., August 7, 2000; 150(3): 613 - 626. [Abstract] [Full Text] [PDF]

				M. Dunckley, K. Wells, T. Piper, D. Wells, and G Dickson Independent localization of dystrophin N- and C-terminal regions to the sarcolemma of mdx mouse myofibres in vivo J. Cell Sci., January 6, 1994; 107(6): 1469 - 1475. [Abstract] [PDF]