spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Emerson, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Emerson, J. A.

Development, Vol 104, Issue 2 219-234, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Disruption of the cytokeratin filament network in the preimplantation mouse embryo

JA Emerson
Department of Anatomy, University of California, San Francisco 94143.

The distribution of the cytokeratin network in the intact preimplantation mouse embryo and the role of cytokeratin filaments in trophectoderm differentiation were investigated by means of whole-mount indirect immunofluorescence microscopy and microinjection of anti-cytokeratin antibody. Assembled cytokeratin filaments were detected in some blastomeres as early as the compacted 8-cell stage. The incidence and organization of cytokeratin filaments increased during the morula stage, although individual blastomeres varied in their content of assembled filaments. At the blastocyst stage, each trophectoderm cell contained an intricate network of cytokeratin filaments, and examination of sectioned blastocysts confirmed that extensive arrays of cytokeratin filaments were restricted to cells of the trophectoderm. Microinjection of anticytokeratin antibody into individual mural trophectoderm cells of expanded blastocysts resulted in a dramatic rearrangement of the cytokeratin network in these cells. Moreover, antibody injection into 2-cell embryos inhibited assembly of the cytokeratin network during the next two days of development. Despite this disruption of cytokeratin assembly, the injected embryos compacted and developed into blastocysts with normal morphology and nuclear numbers. These results suggest that formation of an elaborate cytokeratin network in preimplantation mouse embryos is unnecessary for the initial stages of trophectoderm differentiation resulting in blastocyst formation.


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
Y. Verlinsky and S. Evsikov
Karyotyping of human oocytes by chromosomal analysis of the second polar bodies
Mol. Hum. Reprod., February 1, 1999; 5(2): 89 - 95.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
T. M. Magin, R. Schroder, S. Leitgeb, F. Wanninger, K. Zatloukal, C. Grund, and D. W. Melton
Lessons from Keratin 18 Knockout Mice: Formation of Novel Keratin Filaments, Secondary Loss of Keratin 7 and Accumulation of Liver-specific Keratin 8-Positive Aggregates
J. Cell Biol., March 23, 1998; 140(6): 1441 - 1451.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
H Baribault, J Price, K Miyai, and R G Oshima
Mid-gestational lethality in mice lacking keratin 8.
Genes & Dev., July 1, 1993; 7(7a): 1191 - 1202.
[Abstract] [PDF]


© The Company of Biologists Ltd 1988