Development, Vol 104, Issue 2 297-303, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Prevention of spinal neural tube defects in the mouse embryo by growth retardation during neurulation

AJ Copp, JA Crolla and FA Brook
Department of Zoology, University of Oxford, UK.

Homozygous mutant curly tail mouse embryos developing spinal neural tube defects (NTD) exhibit a cell-type-specific abnormality of cell proliferation that affects the gut endoderm and notochord but not the neuroepithelium. We suggested that spinal NTD in these embryos may result from the imbalance of cell proliferation rates between affected and unaffected cell types. In order to test this hypothesis, curly tail embryos were subjected to influences that retard growth in vivo and in vitro. The expectation was that growth of unaffected rapidly growing cell types would be reduced to a greater extent than affected slowly growing cell types, thus counteracting the genetically determined imbalance of cell proliferation rates and leading to normalization of spinal neurulation. Food deprivation of pregnant females for 48 h prior to the stage of posterior neuropore closure reduced the overall incidence of spinal NTD and almost completely prevented open spina bifida, the most severe form of spinal NTD in curly tail mice. Analysis of embryos earlier in gestation showed that growth retardation acts by reducing the incidence of delayed neuropore closure. Culture of embryos at 40.5 degrees C for 15-23 h from day 10 of gestation, like food deprivation in vivo, also produced growth retardation and led to normalization of posterior neuropore closure. Labelling of embryos in vitro with [3H]thymidine for 1 h at the end of the culture period showed that the labelling index is reduced to a greater extent in the neuroepithelium than in other cell types in growth-retarded embryos compared with controls cultured at 38 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				P. Cogram, A. Hynes, L. P.E. Dunlevy, N. D.E. Greene, and A. J. Copp Specific isoforms of protein kinase C are essential for prevention of folate-resistant neural tube defects by inositol Hum. Mol. Genet., January 1, 2004; 13(1): 7 - 14. [Abstract] [Full Text] [PDF]

				W. Chen, G. Morriss-Kay, and A. Copp Genesis and prevention of spinal neural tube defects in the curly tail mutant mouse: involvement of retinoic acid and its nuclear receptors RAR-beta and RAR-gamma Development, January 3, 1995; 121(3): 681 - 691. [Abstract] [PDF]

				J. Estibeiro, F. Brook, and A. Copp Interaction between splotch (Sp) and curly tail (ct) mouse mutants in the embryonic development of neural tube defects Development, January 9, 1993; 119(1): 113 - 121. [Abstract] [PDF]