spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baldwin, T. J.
Right arrow Articles by Burden, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baldwin, T. J.
Right arrow Articles by Burden, S. J.

Development, Vol 104, Issue 4 557-564, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Regulation of transcript encoding the 43K subsynaptic protein during development and after denervation

TJ Baldwin, JA Theriot, CM Yoshihara and SJ Burden
Biology Department, Massachusetts Institute of Technology, Cambridge 02139.

The postsynaptic membrane of vertebrate neuromuscular synapses is enriched in the four subunits of the acetylcholine receptor (AChR) and in a peripheral membrane protein of Mr = 43 x 10(3) (43K). Although AChRs are virtually restricted to the postsynaptic membrane of innervated adult muscle, developing and denervated adult muscle contain AChRs at nonsynaptic regions. These nonsynaptic AChRs accumulate because the level of mRNA encoding AChR subunits increases in response to a loss of muscle cell electrical activity. We have determined the level of mRNA encoding the 43K subsynaptic protein in developing muscle and in innervated and denervated adult muscle. We isolated a cDNA that encodes the entire protein-coding region of the 43K subsynaptic protein from Torpedo electric organ and used this cDNA to isolate a cDNA that encodes the 43K subsynaptic protein from Xenopus laevis. We used the Xenopus cDNA to measure the level of transcript encoding the 43K protein in embryonic muscle and in innervated and denervated adult muscle by RNase protection. The level of transcript encoding the 43K protein is low in innervated adult muscle and increases 25- to 30-fold after denervation. The level of transcript encoding the alpha subunit of the AChR increases to a similar extent after denervation. Moreover, during development, transcripts encoding the 43K protein and the alpha subunit are expressed initially at late gastrula and are present in similar quantities in embryonic muscle. These results demonstrate that transcripts encoding the 43K protein and AChR subunits appear coordinately during embryonic development and that the level of mRNA encoding the 43K protein is regulated by denervation.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. R. Tremblay and S. Carbonetto
An Extracellular Pathway for Dystroglycan Function in Acetylcholine Receptor Aggregation and Laminin Deposition in Skeletal Myotubes
J. Biol. Chem., May 12, 2006; 281(19): 13365 - 13373.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
H.-O. NGHIÊM, L. BETTENDORFF, and J.-P. CHANGEUX
Specific phosphorylation of Torpedo 43K rapsyn by endogenous kinase(s) with thiamine triphosphate as the phosphate donor
FASEB J, March 1, 2000; 14(3): 543 - 554.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
A. L. Burns, D. Benson, M. J. Howard, and J. F. Margiotta
Chick Ciliary Ganglion Neurons Contain Transcripts Coding for Acetylcholine Receptor-Associated Protein at Synapses (Rapsyn)
J. Neurosci., July 1, 1997; 17(13): 5016 - 5026.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1988