|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 105, Issue 1 61-74, Copyright © 1989 by Company of Biologists
JOURNAL ARTICLES |
JA Dent, AG Polson and MW Klymkowsky
Molecular, Cellular & Developmental Biology, University of Colorado, Boulder 80309-0347.
We have developed a whole-mount immunocytochemical method for Xenopus and used it to map the expression of the intermediate filament protein vimentin during early embryogenesis. We used two monoclonal antibodies, 14h7 and RV202. Both label vimentin filaments in Xenopus A6 cells, RV202 reacts specifically with vimentin (Mr, 55 x 10(3] on Western blots of A6 cells and embryos. 14h7 reacts with vimentin and a second, insoluble polypeptide of 57 x 10(3) Mr found in A6 cells. The 57 x 10(3) Mr polypeptide appears to be an intermediate filament protein immunochemically related to vimentin. In the whole-mount embryo, we first found vimentin at the time of neural tube closure (stage 19) in cells located at the lateral margins of the neural tube. By stage 26, these cells, which are presumably radial glia, are present along the entire length of the neural tube and in the tail bud. Cells in the optic vesicles express vimentin by stage 24. Vimentin-expressing mesenchymal cells appear on the surface of the somites at stage 22/23; these cells appear first on anterior somites and on progressively more posterior somites as development continues. Beginning at stage 24, vimentin appears in mesenchymal cells located ventral to the somites and associated with the pronephric ducts; these ventral cells first appear below the anterior somites and later appear below more posterior somites. The dorsal fin mesenchyme expresses vimentin at stage 26. In the head, both mesodermally-derived and neural-crest-derived mesenchymal tissues express vimentin by stage 26. These include the mesenchyme of the branchial arches, the mandibular arch, the corneal epithelium, the eye, the meninges and mesenchyme surrounding the otic vesicle. By stage 33, vimentin-expressing mesenchymal cells are present in the pericardial cavity and line the vitelline veins. Vimentin expression appears to be a marker for the differentiation of a subset of central nervous system cells and of head and body mesenchyme in the early Xenopus embryo.
This article has been cited by other articles:
|
|
 |
|
  Y. Liu, C. Gervasi, and B. G. Szaro A crucial role for hnRNP K in axon development in Xenopus laevis Development, September 15, 2008; 135(18): 3125 - 3135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Joyner and N. Wall Immunohistochemistry of Whole-Mount Mouse Embryos CSH Protocols, January 1, 2008; 2008(2): pdb.prot4820 - pdb.prot4820. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Ahnfelt-Ronne, M. C. Jorgensen, J. Hald, O. D. Madsen, P. Serup, and J. Hecksher-Sorensen An Improved Method for Three-dimensional Reconstruction of Protein Expression Patterns in Intact Mouse and Chicken Embryos and Organs J. Histochem. Cytochem., September 1, 2007; 55(9): 925 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tao, S. Nandadasa, P. D. McCrea, J. Heasman, and C. Wylie G-protein-coupled signals control cortical actin assembly by controlling cadherin expression in the early Xenopus embryo Development, July 15, 2007; 134(14): 2651 - 2661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Rottbauer, G. Wessels, T. Dahme, S. Just, N. Trano, D. Hassel, C. G. Burns, H. A. Katus, and M. C. Fishman Cardiac Myosin Light Chain-2: A Novel Essential Component of Thick-Myofilament Assembly and Contractility of the Heart Circ. Res., August 4, 2006; 99(3): 323 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson Zebrafish as a Model Vertebrate for Investigating Chemical Toxicity Toxicol. Sci., July 1, 2005; 86(1): 6 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Lowery and H. Sive Initial formation of zebrafish brain ventricles occurs independently of circulation and requires the nagie oko and snakehead/atp1a1a.1 gene products Development, May 1, 2005; 132(9): 2057 - 2067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ralle, C. Grund, W. W. Franke, and R. Stick Intranuclear membrane structure formations by CaaX-containing nuclear proteins J. Cell Sci., December 1, 2004; 117(25): 6095 - 6104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Rubinstein, J. D. Porter, and J. F. Y. Hoh The Development of Longitudinal Variation of Myosin Isoforms in the Orbital Fibers of Extraocular Muscles of Rats Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 3067 - 3072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Hogan, C. A. Ambler, D. L. Chapman, and V. L. Bautch The neural tube patterns vessels developmentally using the VEGF signaling pathway Development, April 1, 2004; 131(7): 1503 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Hill, S. M. Bello, A. L. Prasch, R. E. Peterson, and W. Heideman Water Permeability and TCDD-Induced Edema in Zebrafish Early-Life Stages Toxicol. Sci., March 1, 2004; 78(1): 78 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhang, T. Basta, E. D. Jensen, and M. W. Klymkowsky The {beta}-catenin/VegT-regulated early zygotic gene Xnr5 is a direct target of SOX3 regulation Development, December 1, 2003; 130(23): 5609 - 5624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. Sawtell Quantitative Analysis of Herpes Simplex Virus Reactivation In Vivo Demonstrates that Reactivation in the Nervous System Is Not Inhibited at Early Times Postinoculation J. Virol., April 1, 2003; 77(7): 4127 - 4138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Kroll, W.-m. Zhao, C. Jiang, and P. W. Huber A homolog of FBP2/KSRP binds to localized mRNAs in Xenopus oocytes Development, March 14, 2003; 129(24): 5609 - 5619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lagutina, S. J. Conway, J. Sublett, and G. C. Grosveld Pax3-FKHR Knock-In Mice Show Developmental Aberrations but Do Not Develop Tumors Mol. Cell. Biol., October 15, 2002; 22(20): 7204 - 7216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. V. Sillitoe and R. Hawkes Whole-mount Immunohistochemistry: A High-throughput Screen for Patterning Defects in the Mouse Cerebellum J. Histochem. Cytochem., February 1, 2002; 50(2): 235 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. WOLFF, B. GRUNWALD, B. FRIEDRICH, F. LANG, S. GODEHARDT, and G. BURCKHARDT Cationic Amino Acids Involved in Dicarboxylate Binding of the Flounder Renal Organic Anion Transporter J. Am. Soc. Nephrol., October 1, 2001; 12(10): 2012 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Rubinstein and J. F. Y. Hoh The Distribution of Myosin Heavy Chain Isoforms among Rat Extraocular Muscle Fiber Types Invest. Ophthalmol. Vis. Sci., October 1, 2000; 41(11): 3391 - 3398. [Abstract] [Full Text]
|
|
|
|
|
|
A. Majumdar, K. Lun, M. Brand, and I. A. Drummond Zebrafish no isthmus reveals a role for pax2.1 in tubule differentiation and patterning events in the pronephric primordia Development, May 15, 2000; 127(10): 2089 - 2098. [Abstract] [PDF]
|
|
|
|
 |
|
 U. Schulte, H. Hahn, M. Konrad, N. Jeck, C. Derst, K. Wild, S. Weidemann, J. P. Ruppersberg, B. Fakler, and J. Ludwig pH gating of ROMK (Kir1.1) channels: Control by an Arg-Lys-Arg triad disrupted in antenatal Bartter syndrome PNAS, December 21, 1999; 96(26): 15298 - 15303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Xavier-Neto, C. Neville, M. Shapiro, L Houghton, G. Wang, W Nikovits, F. Stockdale, and N Rosenthal A retinoic acid-inducible transgenic marker of sino-atrial development in the mouse heart Development, January 6, 1999; 126(12): 2677 - 2687. [Abstract] [PDF]
|
|
|
|
|
|
J. Gotz, A. Probst, E. Ehler, B. Hemmings, and W. Kues Delayed embryonic lethality in mice lacking protein phosphatase 2A catalytic subunit Calpha PNAS, October 13, 1998; 95(21): 12370 - 12375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Drummond, A Majumdar, H Hentschel, M Elger, L Solnica-Krezel, A. Schier, S. Neuhauss, D. Stemple, F Zwartkruis, Z Rangini, et al. Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function Development, January 12, 1998; 125(23): 4655 - 4667. [Abstract] [PDF]
|
|
|
|
|
|
B Ferreiro, M Artinger, K Cho, and C Niehrs Antimorphic goosecoids Development, January 4, 1998; 125(8): 1347 - 1359. [Abstract] [PDF]
|
|
|
|
|
|
D Onichtchouk, A Glinka, and C Niehrs Requirement for Xvent-1 and Xvent-2 gene function in dorsoventral patterning of Xenopus mesoderm Development, January 4, 1998; 125(8): 1447 - 1456. [Abstract] [PDF]
|
|
|
|
|
|
G. Serbedzija, J. Chen, and M. Fishman Regulation in the heart field of zebrafish Development, January 3, 1998; 125(6): 1095 - 1101. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Caldero, D. Prevette, X. Mei, R. A. Oakley, L. Li, C. Milligan, L. Houenou, M. Burek, and R. W. Oppenheim Peripheral Target Regulation of the Development and Survival of Spinal Sensory and Motor Neurons in the Chick Embryo J. Neurosci., January 1, 1998; 18(1): 356 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Erickson, K. O'Shea, N Ghaboosi, L Loverro, G Frantz, M Bauer, L. Lu, and M. Moore ErbB3 is required for normal cerebellar and cardiac development: a comparison with ErbB2-and heregulin-deficient mice Development, January 12, 1997; 124(24): 4999 - 5011. [Abstract] [PDF]
|
|
|
|
|
|
M. Lane and R Keller Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by the vegetal alignment zone Development, January 2, 1997; 124(4): 895 - 906. [Abstract] [PDF]
|
|
|
|
|
|
J. Chen, P Haffter, J Odenthal, E Vogelsang, M Brand, F. van Eeden, M Furutani-Seiki, M Granato, M Hammerschmidt, C. Heisenberg, et al. Mutations affecting the cardiovascular system and other internal organs in zebrafish Development, January 12, 1996; 123(1): 293 - 302. [Abstract] [PDF]
|
|
|
|
|
|
Y Chen, M Bei, I Woo, I Satokata, and R Maas Msx1 controls inductive signaling in mammalian tooth morphogenesis Development, January 10, 1996; 122(10): 3035 - 3044. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Lee, S. Hollenberg, L Snider, D. Turner, N Lipnick, and H Weintraub Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein Science, May 12, 1995; 268(5212): 836 - 844. [Abstract] [PDF]
|
|
|
|
|
|
R. Cary and M. Klymkowsky Disruption of intermediate filament organization leads to structural defects at the intersomite junction in Xenopus myotomal muscle Development, January 4, 1995; 121(4): 1041 - 1052. [Abstract] [PDF]
|
|
|
|
|
|
M Pannese, C Polo, M Andreazzoli, R Vignali, B Kablar, G Barsacchi, and E Boncinelli The Xenopus homologue of Otx2 is a maternal homeobox gene that demarcates and specifies anterior body regions Development, January 3, 1995; 121(3): 707 - 720. [Abstract] [PDF]
|
|
|
|
|
|
K. Kroll and J. Gerhart Transgenic X. laevis embryos from eggs transplanted with nuclei of transfected cultured cells Science, October 28, 1994; 266(5185): 650 - 653. [Abstract] [PDF]
|
|
|
|
 |
|
 D L Turner and H Weintraub Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate. Genes & Dev., June 15, 1994; 8(12): 1434 - 1447. [Abstract] [PDF]
|
|
|
|
|
|
R A Rupp, L Snider, and H Weintraub Xenopus embryos regulate the nuclear localization of XMyoD. Genes & Dev., June 1, 1994; 8(11): 1311 - 1323. [Abstract] [PDF]
|
|
|
|
|
|
F Fagotto and B. Gumbiner Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries Development, January 12, 1994; 120(12): 3667 - 3679. [Abstract] [PDF]
|
|
|
|
|
|
R. Cary, M. Klymkowsky, R. Evans, A Domingo, J. Dent, and L. Backhus Vimentin's tail interacts with actin-containing structures in vivo J. Cell Sci., January 6, 1994; 107(6): 1609 - 1622. [Abstract] [PDF]
|
|
|
|
|
|
A Frumkin, G Pillemer, R Haffner, N Tarcic, Y Gruenbaum, and A Fainsod A role for CdxA in gut closure and intestinal epithelia differentiation Development, January 2, 1994; 120(2): 253 - 263. [Abstract] [PDF]
|
|
|
|
|
|
A Frumkin, R Haffner, E Shapira, N Tarcic, Y Gruenbaum, and A Fainsod The chicken CdxA homeobox gene and axial positioning during gastrulation Development, January 6, 1993; 118(2): 553 - 562. [Abstract] [PDF]
|
|
|
|
|
|
S Schneider, K Herrenknecht, S Butz, R Kemler, and P Hausen Catenins in Xenopus embryogenesis and their relation to the cadherin-mediated cell-cell adhesion system Development, January 6, 1993; 118(2): 629 - 640. [Abstract] [PDF]
|
|
|
|
|
|
K. Shimamura and M. Takeichi Local and transient expression of E-cadherin involved in mouse embryonic brain morphogenesis Development, December 1, 1992; 116(4): 1011 - 1019. [Abstract] [PDF]
|
|
|
|
|
|
A Ruiz i Altaba and T Jessell Retinoic acid modifies mesodermal patterning in early Xenopus embryos. Genes & Dev., February 1, 1991; 5(2): 175 - 187. [Abstract] [PDF]
|
|
|
|
|
|
A. Otte, I. Kramer, and A. Durston Protein kinase C and regulation of the local competence of Xenopus ectoderm Science, February 1, 1991; 251(4993): 570 - 573. [Abstract] [PDF]
|
|
