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Development, Vol 106, Issue 1 195-201, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Plasminogen activator expression in F9 teratocarcinoma embryoid bodies and their endoderm derivatives

KR Sabbag, JE Casanova and LB Grabel
Whitehead Institute, 9 Cambridge Center, MA 02142.

Plasminogen activators are believed to play an important role in tissue remodeling and cell migration. During mouse embryogenesis, visceral endoderm secretes urokinase-type plasminogen activator (uPA) whereas parietal endoderm secretes tissue-type plasminogen activator (tPA). Visceral endoderm from F9 embryoid bodies can transdifferentiate into parietal endoderm under the appropriate culture conditions. We have examined at the protein and mRNA levels the type of plasminogen activator expressed in whole embryoid bodies, visceral endoderm and its parietal endoderm derivatives. Our experiments show that the visceral endoderm on F9 embryoid bodies synthesizes and secretes substantial amounts of both tPA and uPA. In contrast, the parietal endoderm derived directly from the visceral endoderm secretes dramatically increased levels of tPA and decreases production of uPA to low or below detectable levels. These data support the finding that visceral endoderm can transdifferentiate to parietal endoderm. In addition, this transition provides an excellent model for studying the molecular basis of the coincident down- and upregulation of the two plasminogen activators as well as their potential function during embryogenesis.


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O Behrendtsen, C. Alexander, and Z Werb
Cooperative interactions between extracellular matrix, integrins and parathyroid hormone-related peptide regulate parietal endoderm differentiation in mouse embryos
Development, January 12, 1995; 121(12): 4137 - 4148.
[Abstract] [PDF]


© The Company of Biologists Ltd 1989