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Development, Vol 106, Issue 3 473-481, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Analysis of specific mRNA destabilization during Dictyostelium development

G Mangiarotti, S Bulfone, R Giorda, P Morandini, A Ceccarelli and BD Hames
Department of Genetics, Biology and Chemistry, University of Turin, Torino, Italy.

A number of specific mRNAs are destabilized upon disaggregation of developing Dictyostelium discoideum cells. Analysis of a family of cloned genes indicates that only prespore-enriched mRNAs are affected; constitutive mRNAs that are expressed throughout development and mRNAs that accumulate preferentially in prestalk cells are stable under these conditions. The decay of sensitive prespore mRNAs can be halted by allowing the cells to reaggregate, indicating that destabilization occurs by the progressive selection of individual molecules rather than on all members of an mRNA subpopulation at the time of disaggregation. Individual molecules of the sensitive mRNA species remain engaged in protein synthesis in the disaggregated cells until selected. Destabilization of sensitive mRNAs is induced by cell dissociation even in the presence of concentrations of nogalamycin that inhibit RNA synthesis. The reported prevention of disaggregation-induced mRNA decay by actinomycin D and daunomycin is therefore probably a secondary effect unrelated to the inhibition of transcription.


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S. Chiaberge, E. Cassarino, and G. Mangiarotti
The Phosphorylation of Protein S6 Modulates the Interaction of the 40 S Ribosomal Subunit with the 5'-Untranslated Region of a Dictyostelium Pre-spore-specific mRNA and Controls Its Stability
J. Biol. Chem., October 16, 1998; 273(42): 27070 - 27075.
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© The Company of Biologists Ltd 1989