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Development, Vol 106, Issue 4 635-640, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Cell proliferation and expression of cytokeratin filaments in F9 embryonal carcinoma cells

P Kurki, A Laasonen, EM Tan and E Lehtonen
Department of Pathology, University of Helsinki, Finland.

A double immunofluorescence method was developed for the monitoring of proliferation and differentiation of F9 embryonal carcinoma cells. Cytokeratin filament expression was used as a marker for differentiation, and proliferating cell nuclear antigen (PCNA)/cyclin or bromodeoxyuridine labeling were used as markers for proliferation. F9 cells had a high proliferation rate and were cytokeratin-filament-negative. Upon treatment with retinoic acid and dibutyryl cyclic AMP, cytokeratin-filament-positive cells with differentiated phenotype appeared. After 3 days, the extent of proliferation of cytokeratin-filament-positive cells was comparable to, but after 5 days significantly lower than, that of cytokeratin-filament-negative cells in the same culture. In differentiating F9 cells, cytokeratin filament expression is associated with, and even slightly precedes, the dramatic decrease in the rate of proliferation.


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© The Company of Biologists Ltd 1989