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Development, Vol 106, Issue 4 675-683, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Expression of N-CAM precedes neural induction in Pleurodeles waltl (urodele, amphibian)

JP Saint-Jeannet, F Foulquier, C Goridis and AM Duprat
U.A. CNRS 675, Centre de biologie du developpement, Universite Paul Sabatier, Toulouse, France.

The appearance and localization of N-CAM during neural induction were studied in Pleurodeles waltl embryos and compared with recent contradictory results reported in Xenopus laevis. A monoclonal antibody raised against mouse N-CAM was used. In the nervous system of Pleurodeles, it recognized two glycoproteins of 180 and 140x10(3) M(r) which are the Pleurodeles equivalent of N-CAM-180 and -140. Using this probe for immunohistochemistry and immunocytochemistry, we showed that N-CAM was already expressed in presumptive ectoderm at the early gastrula stage. In late gastrula embryos, a slight increase in staining was observed in the neurectoderm, whereas the labelling persisted in the noninduced ectoderm. When induced ectodermal cells were isolated at the late gastrula stage and cultured in vitro up to 14 days, a faint polarized labelling of cells was observed initially. During differentiation, the staining increased and became progressively restricted to differentiating neurons.


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L Bally-Cuif, C Goridis, and M. Santoni
The mouse NCAM gene displays a biphasic expression pattern during neural tube development
Development, January 2, 1993; 117(2): 543 - 552.
[Abstract] [PDF]


© The Company of Biologists Ltd 1989