spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kloc, M.
Right arrow Articles by Etkin, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kloc, M.
Right arrow Articles by Etkin, L.

Development, Vol 107, Issue 4 899-907, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

The maternal store of the xlgv7 mRNA in full-grown oocytes is not required for normal development in Xenopus

M Kloc, M Miller, AE Carrasco, E Eastman and L Etkin
Department of Molecular Genetics, University of Texas MD Anderson Cancer Center, Houston 77030.

We have attempted to analyze the function of a maternal mRNA xlgv7 which is distributed as an animal-vegetal gradient in stage 6 oocytes using a combination of antisense oligodeoxynucleotide injection into oocytes followed by in vitro maturation and fertilization. Injection of 20 ng of the antisense oligodeoxynucleotide resulted in the destruction of the xlgv7 mRNA to undetectable levels. Upon maturation and fertilization the resulting embryos develop with no specific defects suggesting that the maternal store of xlgv7 in stage 6 oocytes is not required and that the embryo can develop solely with the maternal store of the xlgv7 protein. Also, these results demonstrate the feasibility of this approach in destroying a specific maternal RNA and assaying its effect on development.


This article has been cited by other articles:


Home page
 ScienceHome page
M Kloc and L. Etkin
Delocalization of Vg1 mRNA from the vegetal cortex in Xenopus oocytes after destruction of Xlsirt RNA
Science, August 19, 1994; 265(5175): 1101 - 1103.
[Abstract] [PDF]

Home page
 DevelopmentHome page
J Heasman, D Ginsberg, B Geiger, K Goldstone, T Pratt, C Yoshida-Noro, and C Wylie
A functional test for maternally inherited cadherin in Xenopus shows its importance in cell adhesion at the blastula stage
Development, January 1, 1994; 120(1): 49 - 57.
[Abstract] [PDF]


© The Company of Biologists Ltd 1989