Development, Vol 107, Issue 4 945-956, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Transactivation of the adenovirus EIIa promoter in the absence of adenovirus E1A protein is restricted to mouse oocytes and preimplantation embryos

TP Dooley, M Miranda, NC Jones and ML DePamphilis
Gene Regulation Laboratory, Imperial Cancer Research Fund, London, England.

Undifferentiated mouse embryonal carcinoma (EC) cells are capable of transactivating the adenovirus EIIa promoter in the absence of its normal transactivator, E1A protein, suggesting that EC cells contain an E1A-like activity. In an effort to identify where this activity appears during normal mouse development, mouse oocytes and preimplantation embryos were injected with plasmids containing the EIIa promoter coupled to various reporter genes. These expression vectors were fully active in human 293 cells where E1A is present, but were inactive in differentiated fibroblast cell lines unless cotransfected with the E1A gene. In mouse oocytes and preimplantation embryos, EIIa promoter activity in the absence of adenovirus E1A protein was equivalent to or greater than activity of the HSV thymidine kinase promoter coupled to a strong enhancer. Coinjection of the E1A gene failed to stimulate EIIa activity further, perhaps because c-myc protein, which has been reported to transactivate this promoter, was already present at high levels in mouse oocytes. Activation of the EIIa promoter in the absence of E1A was unique to mouse oocytes and preimplantation embryos because gene expression from an EIIa promoter introduced into transgenic mice was observed only in the adult ovary, and particularly in the oocytes. In addition, post-implantation transgenic embryos failed to express the E1A-activatable reporter gene, thereby indicating that expression from the EIIa promoter is restricted to the relatively undifferentiated stages of oogenesis and preimplantation development. These data suggest that cellular promoters of the class that can be transactivated by E1A may serve uniquely to initiate transcription of genes that are needed for preimplantation development.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				Y. A. Minamishima, J. Moslehi, N. Bardeesy, D. Cullen, R. T. Bronson, and W. G. Kaelin Jr Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure Blood, March 15, 2008; 111(6): 3236 - 3244. [Abstract] [Full Text] [PDF]

				M. Y. Chang, J. Boulden, J. B. Katz, L. Wang, T. J. Meyer, A. P. Soler, A. J. Muller, and G. C. Prendergast Bin1 Ablation Increases Susceptibility to Cancer during Aging, Particularly Lung Cancer Cancer Res., August 15, 2007; 67(16): 7605 - 7612. [Abstract] [Full Text] [PDF]

				A. Diwan, A. G. Koesters, A. M. Odley, S. Pushkaran, C. P. Baines, B. T. Spike, D. Daria, A. G. Jegga, H. Geiger, B. J. Aronow, et al. Unrestrained erythroblast development in Nix-/- mice reveals a mechanism for apoptotic modulation of erythropoiesis PNAS, April 17, 2007; 104(16): 6794 - 6799. [Abstract] [Full Text] [PDF]

				S. J. Matkovich, A. Diwan, J. L. Klanke, D. J. Hammer, Y. Marreez, A. M. Odley, E. W. Brunskill, W. J. Koch, R. J. Schwartz, and G. W. Dorn II Cardiac-Specific Ablation of G-Protein Receptor Kinase 2 Redefines Its Roles in Heart Development and {beta}-Adrenergic Signaling Circ. Res., October 27, 2006; 99(9): 996 - 1003. [Abstract] [Full Text] [PDF]

				M. Tomioka, M. Nishimoto, S. Miyagi, T. Katayanagi, N. Fukui, H. Niwa, M. Muramatsu, and A. Okuda Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex Nucleic Acids Res., July 15, 2002; 30(14): 3202 - 3213. [Abstract] [Full Text] [PDF]

				M. Holzenberger, C. Lenzner, P. Leneuve, R. Zaoui, G. Hamard, S. Vaulont, and Y. L. Bouc Cre-mediated germline mosaicism: a method allowing rapid generation of several alleles of a target gene Nucleic Acids Res., November 1, 2000; 28(21): e92 - e92. [Abstract] [Full Text] [PDF]

				K. E. Latham, B. Patel, F. D. M. Bautista, and S. M. Hawes Effects of X Chromosome Number and Parental Origin on X-Linked Gene Expression in Preimplantation Mouse Embryos Biol Reprod, July 1, 2000; 63(1): 64 - 73. [Abstract] [Full Text]

				Q. Wang and K. E. Latham Translation of Maternal Messenger Ribonucleic Acids Encoding Transcription Factors During Genome Activation in Early Mouse Embryos Biol Reprod, April 1, 2000; 62(4): 969 - 978. [Abstract] [Full Text]

				Y. Fujitani, Y. Kajimoto, T. Yasuda, T.-A. Matsuoka, H. Kaneto, Y. Umayahara, N. Fujita, H. Watada, J.-I. Miyazaki, Y. Yamasaki, et al. Identification of a Portable Repression Domain and an E1A-Responsive Activation Domain in Pax4: a Possible Role of Pax4 as a Transcriptional Repressor in the Pancreas Mol. Cell. Biol., December 1, 1999; 19(12): 8281 - 8291. [Abstract] [Full Text] [PDF]

				G. Tremml, C. Dominguez, V. Rosti, Z. Zhang, P.P. Pandolfi, P. Keller, and M. Bessler Increased Sensitivity to Complement and a Decreased Red Blood Cell Life Span in Mice Mosaic for a Nonfunctional Piga Gene Blood, November 1, 1999; 94(9): 2945 - 2954. [Abstract] [Full Text] [PDF]

				M. Nishimoto, A. Fukushima, A. Okuda, and M. Muramatsu The Gene for the Embryonic Stem Cell Coactivator UTF1 Carries a Regulatory Element Which Selectively Interacts with a Complex Composed of Oct-3/4 and Sox-2 Mol. Cell. Biol., August 1, 1999; 19(8): 5453 - 5465. [Abstract] [Full Text] [PDF]

				P. Keller, G. Tremml, V. Rosti, and M. Bessler X inactivation and somatic cell selection rescue female mice carrying a Piga-null mutation PNAS, June 22, 1999; 96(13): 7479 - 7483. [Abstract] [Full Text] [PDF]

				P. Lawinger, L. Rastelli, Z. Zhao, and S. Majumder Lack of Enhancer Function in Mammals Is Unique to Oocytes and Fertilized Eggs J. Biol. Chem., March 19, 1999; 274(12): 8002 - 8011. [Abstract] [Full Text] [PDF]

				J.-L. Liu, A. Grinberg, H. Westphal, B. Sauer, D. Accili, M. Karas, and D. LeRoith Insulin-Like Growth Factor-I Affects Perinatal Lethality and Postnatal Development in a Gene Dosage-Dependent Manner: Manipulation Using the Cre/loxP System in Transgenic Mice Mol. Endocrinol., September 1, 1998; 12(9): 1452 - 1462. [Abstract] [Full Text]

				M Wiekowski, M Miranda, J. Nothias, and M. DePamphilis Changes in histone synthesis and modification at the beginning of mouse development correlate with the establishment of chromatin mediated repression of transcription J. Cell Sci., January 5, 1997; 110(10): 1147 - 1158. [Abstract] [PDF]

				K. Kaneko, E. Cullinan, K. Latham, and M. DePamphilis Transcription factor mTEAD-2 is selectively expressed at the beginning of zygotic gene expression in the mouse Development, January 5, 1997; 124(10): 1963 - 1973. [Abstract] [PDF]

				M. J. Quinones, J. Leor, R. A. Kloner, M. Ito, M. Patterson, W. F. Witke, and L. Kedes Avoidance of Immune Response Prolongs Expression of Genes Delivered to the Adult Rat Myocardium by Replication-Defective Adenovirus Circulation, September 15, 1996; 94(6): 1394 - 1401. [Abstract] [Full Text]

				J.-Y. Nothias, S. Majumder, K. J. Kaneko, and M. L. DePamphilis Regulation of Gene Expression at the Beginning of Mammalian Development J. Biol. Chem., September 22, 1995; 270(38): 22077 - 22080. [Full Text] [PDF]