|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 110, Issue 1 41-50, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
BR Hough-Evans, RR Franks, RW Zeller, RJ Britten and EH Davidson
Division of Biology, California Institute of Technology, Pasadena 91125.
The CyIIIa.CAT fusion gene was injected into Strongylocentrotus purpuratus eggs, together with excess ligated competitor sequences representing subregions of the CyIIIa regulatory domain. In this construct, the chloramphenicol acetyltransferase (CAT) reporter gene is placed under the control of the 2300 nucleotide upstream regulatory domain of the lineage-specific CyIIIa cytoskeletal actin gene. CAT mRNA was detected by in situ hybridization in serial sections of pluteus stage embryos derived from the injected eggs. When carrier DNA lacking competitor CyIIIa fragments was coinjected with CyIIIa.CAT, CAT mRNA was observed exclusively in aboral ectoderm cells, i.e. the territory in which the CyIIIa gene itself is normally expressed (as also reported by us previously). The same result was obtained when five of seven different competitor subfragments bearing sites of DNA-protein interaction were coinjected. However, coinjection of excess quantities of either of two widely separated, nonhomologous fragments of the CyIIIa regulatory domain produced a dramatic ectopic expression of CAT mRNA in the recipient embryos. CAT mRNA was observed in gut, mesenchyme cells and oral ectoderm in these embryos. We conclude that these fragments contain regulatory sites that negatively control spatial expression of the CyIIIa gene.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Tilton and R. L. Tanguay Exposure to Sodium Metam during Zebrafish Somitogenesis Results in Early Transcriptional Indicators of the Ensuing Neuronal and Muscular Dysfunction Toxicol. Sci., November 1, 2008; 106(1): 103 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Bogarad, M. I. Arnone, C. Chang, and E. H. Davidson Interference with gene regulation in living sea urchin embryos: Transcription factor Knock Out (TKO), a genetically controlled vector for blockade of specific transcription factors PNAS, December 8, 1998; 95(25): 14827 - 14832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Davidson, R. Cameron, and A Ransick Specification of cell fate in the sea urchin embryo: summary and some proposed mechanisms Development, January 9, 1998; 125(17): 3269 - 3290. [Abstract] [PDF]
|
|
|
|
|
|
M. Arnone, E. Martin, and E. Davidson Cis-regulation downstream of cell type specification: a single compact element controls the complex expression of the CyIIa gene in sea urchin embryos Development, January 4, 1998; 125(8): 1381 - 1395. [Abstract] [PDF]
|
|
|
|
|
|
J. Coffman, C. Kirchhamer, M. Harrington, and E. Davidson SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos Development, January 12, 1997; 124(23): 4717 - 4727. [Abstract] [PDF]
|
|
|
|
|
|
M. Arnone and E. Davidson The hardwiring of development: organization and function of genomic regulatory systems Development, January 5, 1997; 124(10): 1851 - 1864. [Abstract] [PDF]
|
|
|
|
|
|
C. Kirchhamer and E. Davidson Spatial and temporal information processing in the sea urchin embryo: modular and intramodular organization of the CyIIIa gene cis-regulatory system Development, January 1, 1996; 122(1): 333 - 348. [Abstract] [PDF]
|
|
|
|
|
|
D. Wang, C. Kirchhamer, R. Britten, and E. Davidson SpZ12-1, a negative regulator required for spatial control of the territory-specific CyIIIa gene in the sea urchin embryo Development, January 4, 1995; 121(4): 1111 - 1122. [Abstract] [PDF]
|
|
|
|
|
|
E. Davidson Later embryogenesis: regulatory circuitry in morphogenetic fields Development, January 7, 1993; 118(3): 665 - 690. [Abstract] [PDF]
|
|
|
|
 |
|
 E. Aamodt, M. Chung, and J. McGhee Spatial control of gut-specific gene expression during Caenorhabditis elegans development Science, April 26, 1991; 252(5005): 579 - 582. [Abstract] [PDF]
|
|
