|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 110, Issue 2 379-384, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
O Ohneda, N Yanai and M Obinata
Department of Cell Biology, Tohoku University, Sendai, Japan.
Mouse stromal cell lines (FLS lines), established from the livers of 13-day gestation mouse fetus, supported the proliferation and differentiation of the erythroid progenitor cells from mouse fetal livers and bone marrow in a semisolid medium in the presence of erythropoietin. A large erythroid colony of over 1000 benzidine-positive erythroid cells was developed from a single erythroid progenitor cell on the FLS cell layer after 4 days of culture. When in close contact with the layer, the erythroid progenitor cells divided rapidly with an average generation time of 9.6 h and mature erythroid cells, including enucleated erythrocytes, were produced. The present studies demonstrate that the microenvironment created by the stromal cells can support the rapid expansion of erythropoietic cell population in the fetal liver of mice.
This article has been cited by other articles:
|
|
 |
|
  T. Yamashita, O. Ohneda, A. Sakiyama, F. Iwata, K. Ohneda, and Y. Fujii-Kuriyama The microenvironment for erythropoiesis is regulated by HIF-2{alpha} through VCAM-1 in endothelial cells Blood, August 15, 2008; 112(4): 1482 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Umeda, T. Heike, M. Nakata-Hizume, A. Niwa, M. Arai, G. Shinoda, F. Ma, H. Suemori, H. Y. Luo, D. H. K. Chui, et al. Sequential Analysis of {alpha}- and {beta}-Globin Gene Expression During Erythropoietic Differentiation from Primate Embryonic Stem Cells Stem Cells, December 1, 2006; 24(12): 2627 - 2636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Y. Mukai, H. Motohashi, O. Ohneda, N. Suzuki, M. Nagano, and M. Yamamoto Transgene Insertion in Proximity to thec-myb Gene Disrupts Erythroid-Megakaryocytic Lineage Bifurcation Mol. Cell. Biol., November 1, 2006; 26(21): 7953 - 7965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Subramanian, H. E. Teal, P. H. Correll, and R. F. Paulson Resistance to Friend Virus-Induced Erythroleukemia in W/Wv Mice Is Caused by a Spleen-Specific Defect Which Results in a Severe Reduction in Target Cells and a Lack of Sf-Stk Expression J. Virol., December 1, 2005; 79(23): 14586 - 14594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Lenox, J. M. Perry, and R. F. Paulson BMP4 and Madh5 regulate the erythroid response to acute anemia Blood, April 1, 2005; 105(7): 2741 - 2748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nakano, K. Ohneda, H. Yamamoto-Mukai, R. Shimizu, O. Ohneda, S. Ohmura, M. Suzuki, S. Tsukamoto, T. Yanagawa, H. Yoshida, et al. Transgenic over-expression of GATA-1 mutant lacking N-finger domain causes hemolytic syndrome in mouse erythroid cells Genes Cells, January 1, 2005; 10(1): 47 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Shimizu, T. Kuroha, O. Ohneda, X. Pan, K. Ohneda, S. Takahashi, S. Philipsen, and M. Yamamoto Leukemogenesis Caused by Incapacitated GATA-1 Function Mol. Cell. Biol., December 15, 2004; 24(24): 10814 - 10825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. O'Prey, N. Leslie, K. Itoh, W. Ostertag, C. Bartholomew, and P. R. Harrison Both Stroma and Stem Cell Factor Maintain Long-Term Growth of ELM Erythroleukemia Cells, but Only Stroma Prevents Erythroid Differentiation in Response to Erythropoietin and Interleukin-3 Blood, March 1, 1998; 91(5): 1548 - 1555. [Abstract] [Full Text] [PDF]
|
|
