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Development, Vol 110, Issue 2 483-489, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

Treatment with beta bungarotoxin blocks muscle spindle formation in fetal rats

J Kucera and JM Waldro
Department of Neurology, School of Medicine, Boston University, Massachusetts 02118.

Sensory and motor fibers of peripheral nerves were irreversibly destroyed in fetal rats by administering beta bungarotoxin (BTX) on embryonic day 16 or 17, after assembly of primary myotubes, but before the formation of muscle spindles. Soleus muscles of toxin-treated fetuses and their untreated littermates were removed just prior to birth and were examined by light microscopy of serial transverse sections for the presence of spindles and immunocytochemical expression of several isoforms of myosin heavy chains (MHC). Untreated muscles exhibited numerous spindles that were innervated by branches of intramuscular nerves and contained muscle fibers expressing a slow-tonic MHC isoform characteristic of the intrafusal but not extrafusal fibers. Toxin-treated muscles were devoid of intramuscular nerve bundles and perineurial structures. Encapsulations of muscle fibers resembling spindles were absent and no myotubes expressed the slow-tonic MHC isoform associated with intrafusal fibers in beta BTX-treated muscles. Thus, the assembly of muscle spindles, formation of the spindle capsule, and transformation of undifferentiated myotubes into the intrafusal fibers that contain spindle-specific myosin isoforms all depend on the presence of innervation in prenatal rat muscles.


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F. Picquet, L. De-Doncker, and M. Falempin
Expression of Myosin Heavy Chain Isoforms in Rat Soleus Muscle Spindles After 19 Days of Hypergravity
J. Histochem. Cytochem., November 1, 2003; 51(11): 1479 - 1489.
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© The Company of Biologists Ltd 1990