spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Knight, J. K.
Right arrow Articles by Raymond, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knight, J. K.
Right arrow Articles by Raymond, P. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 110, Issue 4 1115-1120, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

Time course of opsin expression in developing rod photoreceptors

JK Knight and PA Raymond
Neuroscience Program, University of Michigan, Ann Arbor 48109-0616.

We have investigated the time course of rod photoreceptor determination in the goldfish retina. Rod precursor cells located in the outer nuclear layer of the mature retina continuously generate rod photoreceptors. In this study, we asked when rod precursor cells begin to express opsin, which would signal their commitment to the rod pathway of differentiation. There are three possibilities: a rod precursor could express opsin while still mitotic, at or shortly after the terminal mitosis but before differentiation, or during differentiation. We used immunocytochemistry with antibodies against bromodeoxyuridine, BrdU (a thymidine analogue) and against opsin to determine when during the mitotic history of a cell the expression of opsin first occurred, taking a double labelled cell to be evidence of commitment to the rod cell fate. We found that the first double labelled cells appeared at 4 days after BrdU injection. The number of double labelled cells increased to peak at 10 days, and then fell. These results support the hypothesis that dividing rod precursor cells are probably multipotent stem cells not committed to the rod cell fate.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
O. Yaron, C. Farhy, T. Marquardt, M. Applebury, and R. Ashery-Padan
Notch1 functions to suppress cone-photoreceptor fate specification in the developing mouse retina
Development, April 1, 2006; 133(7): 1367 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Norrander, M. Larsson, S. Stahl, C. Hoog, and R. Linck
Expression of Ciliary Tektins in Brain and Sensory Development
J. Neurosci., November 1, 1998; 18(21): 8912 - 8918.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. M. Levine, H. Roelink, J. Turner, and T. A. Reh
Sonic Hedgehog Promotes Rod Photoreceptor Differentiation in Mammalian Retinal Cells In Vitro
J. Neurosci., August 15, 1997; 17(16): 6277 - 6288.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C Neophytou, A. Vernallis, A Smith, and M. Raff
Muller-cell-derived leukaemia inhibitory factor arrests rod photoreceptor differentiation at a postmitotic pre-rod stage of development
Development, January 6, 1997; 124(12): 2345 - 2354.
[Abstract] [PDF]

Home page
 DevelopmentHome page
Z. Ezzeddine, X Yang, T DeChiara, G Yancopoulos, and C. Cepko
Postmitotic cells fated to become rod photoreceptors can be respecified by CNTF treatment of the retina
Development, January 3, 1997; 124(5): 1055 - 1067.
[Abstract] [PDF]


© The Company of Biologists Ltd 1990