Development, Vol 111, Issue 4 1143-1154, Copyright © 1991 by Company of Biologists

JOURNAL ARTICLES

Differentiating and mature neurons express the acidic fibroblast growth factor gene during chick neural development

H Schnurch and W Risau
Max-Planck-Institut fur Psychiatrie, Abteilung Neurochemie, Martinsried, FRG.

We have previously isolated and characterized acidic fibroblast growth factor (aFGF) from the embryonic chick brain. To analyze the expression of the gene encoding this growth factor a cDNA clone was isolated. The predicted amino acid sequence was found to be highly conserved (90%) between human and chick. Using single-stranded DNA probes, aFGF gene expression was detectable at day 3.5 in the embryonic chick brain. The mRNA level of the 1.7 kb transcript increased during embryonic development and reached the highest level in the adult brain. In situ hybridization results confirmed these developmental changes and revealed a localized expression in neurons. In the adult, Purkinje cells, deep cerebellar and brainstem neurons showed a high level of aFGF mRNA. In the embryonic brain, localized expression in neurons was detectable from day 6 onward. aFGF mRNA was also present in neurons of the peripheral nervous system. In dorsal root ganglia, aFGF was found to be expressed after embryonic day 6. Cells of blood vessels and the ependyma did not express detectable amounts of aFGF mRNA. These results suggest that aFGF may have a function as a differentiation or maintenance factor for postmitotic neurons or as a growth or differentiation factor for other cells in the nervous system mainly in later stages of development.

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