|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 112, Issue 1 279-287, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
AW Puschel, R Balling and P Gruss
Max-Planck-Institut fur biophysikalische Chemie, Abteilung Molekulare Zellbiologie, Gottingen, FRG.
The Hox genes are a class of putative developmental control genes that are thought to be involved in the specification of positional identity along the anteroposterior axis of the vertebrate embryo. It is apparent from their expression pattern that their regulation is dependent upon positional information. In a previous analysis of the Hox-1.1 promoter in transgenic mice, we identified sequences that were sufficient to establish transgene expression in a specific region of the embryo. The construct used, however, did not contain enough regulatory sequences to reproduce all aspects of Hox-1.1 expression. In particular, neither a posterior boundary nor a restriction of expression to prevertebrae was achieved. Here we show correct regulation by Hox-1.1 sequences in transgenic mice and identify the elements responsible for different levels of control. Concomitant with the subdivision of mesodermal cells into different lineages during gastrulation and organogenesis, Hox-1.1 expression is restricted to successively smaller sets of cells. Distinct elements are required at different stages of development to execute this developmental programme. One position-responsive element (130 bp nontranslated leader) was shown to be crucial for the restriction of expression not only along the anteroposterior axis of the embryo, setting the posterior border, but also along the dorsoventral axis of the neural tube and to the lineage giving rise to the prevertebrae. Thus, Hox-1.1 expression is established in a specific region of the embryo and in a specific lineage of the mesoderm by restricting the activity of the promoter by the combined effect of several regulatory elements.
This article has been cited by other articles:
|
|
 |
|
  C Fritsch, J. Brown, J. Kassis, and J Muller The DNA-binding polycomb group protein pleiohomeotic mediates silencing of a Drosophila homeotic gene Development, January 9, 1999; 126(17): 3905 - 3913. [Abstract] [PDF]
|
|
|
|
 |
|
 B. D. Yu, R. D. Hanson, J. L. Hess, S. E. Horning, and S. J. Korsmeyer MLL, a mammalian trithorax-group gene, functions as a transcriptional maintenance factor in morphogenesis PNAS, September 1, 1998; 95(18): 10632 - 10636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Packer, D. Crotty, V. Elwell, and D. Wolgemuth Expression of the murine Hoxa4 gene requires both autoregulation and a conserved retinoic acid response element Development, January 6, 1998; 125(11): 1991 - 1998. [Abstract] [PDF]
|
|
|
|
|
|
T. E. Haerry and W. J. Gehring Intron of the mouse Hoxa-7 gene contains conserved homeodomain binding sites that can functionas an enhancer element in Drosophila PNAS, November 26, 1996; 93(24): 13884 - 13889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Zeltser, C Desplan, and N Heintz Hoxb-13: a new Hox gene in a distant region of the HOXB cluster maintains colinearity Development, January 8, 1996; 122(8): 2475 - 2484. [Abstract] [PDF]
|
|
|
|
|
|
A Morrison, M. Moroni, L Ariza-McNaughton, R Krumlauf, and F Mavilio In vitro and transgenic analysis of a human HOXD4 retinoid-responsive enhancer Development, January 6, 1996; 122(6): 1895 - 1907. [Abstract] [PDF]
|
|
|
|
|
|
T Akasaka, M Kanno, R Balling, M. Mieza, M Taniguchi, and H Koseki A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton Development, January 5, 1996; 122(5): 1513 - 1522. [Abstract] [PDF]
|
|
|
|
|
|
J Muller, S Gaunt, and P. Lawrence Function of the Polycomb protein is conserved in mice and flies Development, January 9, 1995; 121(9): 2847 - 2852. [Abstract] [PDF]
|
|
|
|
|
|
M Frasch, X Chen, and T Lufkin Evolutionary-conserved enhancers direct region-specific expression of the murine Hoxa-1 and Hoxa-2 loci in both mice and Drosophila Development, January 4, 1995; 121(4): 957 - 974. [Abstract] [PDF]
|
|
|
|
|
|
T Knittel, M Kessel, M. Kim, and P Gruss A conserved enhancer of the human and murine Hoxa-7 gene specifies the anterior boundary of expression during embryonal development Development, January 4, 1995; 121(4): 1077 - 1088. [Abstract] [PDF]
|
|
|
|
 |
|
 L Jeannotte, M Lemieux, J Charron, F Poirier, and E J Robertson Specification of axial identity in the mouse: role of the Hoxa-5 (Hox1.3) gene. Genes & Dev., November 1, 1993; 7(11): 2085 - 2096. [Abstract] [PDF]
|
|
|
|
|
|
R Vogels, J Charite, W de Graaff, and J Deschamps Proximal cis-acting elements cooperate to set Hoxb-7 (Hox-2.3) expression boundaries in transgenic mice Development, January 5, 1993; 118(1): 71 - 82. [Abstract] [PDF]
|
|
|
|
|
|
R. Behringer, D. Crotty, V. Tennyson, R. Brinster, R. Palmiter, and D. Wolgemuth Sequences 5' of the homeobox of the Hox-1.4 gene direct tissue-specific expression of lacZ during mouse development Development, January 3, 1993; 117(3): 823 - 833. [Abstract] [PDF]
|
|
|
|
|
|
C Logan, W. Khoo, D Cado, and A. Joyner Two enhancer regions in the mouse En-2 locus direct expression to the mid/hindbrain region and mandibular myoblasts Development, January 3, 1993; 117(3): 905 - 916. [Abstract] [PDF]
|
|
|
|
|
|
M Westerfield, J Wegner, B G Jegalian, E M DeRobertis, and A W Puschel Specific activation of mammalian Hox promoters in mosaic transgenic zebrafish. Genes & Dev., April 1, 1992; 6(4): 591 - 598. [Abstract] [PDF]
|
|
|
|
|
|
J Whiting, H Marshall, M Cook, R Krumlauf, P W Rigby, D Stott, and R K Allemann Multiple spatially specific enhancers are required to reconstruct the pattern of Hox-2.6 gene expression. Genes & Dev., November 1, 1991; 5(11): 2048 - 2059. [Abstract] [PDF]
|
|
