|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 113, Issue 1 245-255, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
M Van Doren, HM Ellis and JW Posakony
Department of Biology, University of California San Diego, La Jolla 92093-0322.
In Drosophila, a group of regulatory proteins of the helix-loop-helix (HLH) class play an essential role in conferring upon cells in the developing adult epidermis the competence to give rise to sensory organs. Proteins encoded by the daughterless (da) gene and three genes of the achaete-scute complex (AS-C) act positively in the determination of the sensory organ precursor cell fate, while the extramacrochaetae (emc) and hairy (h) gene products act as negative regulators. In the region upstream of the achaete gene of the AS-C, we have identified three 'E box' consensus sequences that are bound specifically in vitro by hetero-oligomeric complexes consisting of the da protein and an AS-C protein. We have used this DNA-binding activity to investigate the biochemical basis of the negative regulatory function of emc. Under the conditions of our experiments, the emc protein, but not the h protein, is able to antagonize specifically the in vitro DNA-binding activity of da/AS-C and putative da/da protein complexes. We interpret these results as follows: the heterodimerization capacity of the emc protein (conferred by its HLH domain) allows it to act in vivo as a competitive inhibitor of the formation of functional DNA-binding protein complexes by the da and AS-C proteins, thereby reducing the effective level of their transcriptional regulatory activity within the cell.
This article has been cited by other articles:
|
|
 |
|
  T. Ikawa, H. Kawamoto, A. W. Goldrath, and C. Murre E proteins and Notch signaling cooperate to promote T cell lineage specification and commitment J. Exp. Med., May 15, 2006; 203(5): 1329 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Acar, H. Jafar-Nejad, N. Giagtzoglou, S. Yallampalli, G. David, Y. He, C. Delidakis, and H. J. Bellen Senseless physically interacts with proneural proteins and functions as a transcriptional co-activator Development, May 15, 2006; 133(10): 1979 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Vanolst, C. Fromental-Ramain, and P. Ramain Toutatis, a TIP5-related protein, positively regulates Pannier function during Drosophila neural development Development, October 1, 2005; 132(19): 4327 - 4338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lamar and C. Kintner The Notch targets Esr1 and Esr10 are differentially regulated in Xenopus neural precursors Development, August 15, 2005; 132(16): 3619 - 3630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Adam and D. J. Montell A role for extra macrochaetae downstream of Notch in follicle cell differentiation Development, December 1, 2004; 131(23): 5971 - 5980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tapanes-Castillo and M. K. Baylies Notch signaling patterns Drosophila mesodermal segments by regulating the bHLH transcription factor twist Development, May 15, 2004; 131(10): 2359 - 2372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Karp and I. Greenwald Post-transcriptional regulation of the E/Daughterless ortholog HLH-2, negative feedback, and birth order bias during the AC/VU decision in C. elegans Genes & Dev., December 15, 2003; 17(24): 3100 - 3111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Louis, L. Holm, L. Sanchez, and M. Kaufman A Theoretical Model for the Regulation of Sex-lethal, a Gene That Controls Sex Determination and Dosage Compensation in Drosophila melanogaster Genetics, November 1, 2003; 165(3): 1355 - 1384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Cadigan, A. D. Jou, and R. Nusse Wingless blocks bristle formation and morphogenetic furrow progression in the eye through repression of Daughterless Development, March 9, 2003; 129(14): 3393 - 3402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Frankfort and G. Mardon R8 development in the Drosophila eye: a paradigm for neural selection and differentiation Development, March 5, 2003; 129(6): 1295 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Heitzler, L. Vanolst, I. Biryukova, and P. Ramain Enhancer-promoter communication mediated by Chip during Pannier-driven proneural patterning is regulated by Osa Genes & Dev., March 1, 2003; 17(5): 591 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Barolo and J. W. Posakony Three habits of highly effective signaling pathways: principles of transcriptional control by developmental cell signaling Genes & Dev., May 15, 2002; 16(10): 1167 - 1181. [Full Text] [PDF]
|
|
|
|
|
|
A. Baonza and M. Freeman Notch signalling and the initiation of neural development in the Drosophila eye Development, October 15, 2001; 128(20): 3889 - 3898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Castanon, S. Von Stetina, J. Kass, and M. K. Baylies Dimerization partners determine the activity of the Twist bHLH protein during Drosophila mesoderm development Development, August 15, 2001; 128(16): 3145 - 3159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Ledent and M. Vervoort The Basic Helix-Loop-Helix Protein Family: Comparative Genomics and Phylogenetic Analysis Genome Res., May 1, 2001; 11(5): 754 - 770. [Abstract] [Full Text]
|
|
|
|
|
|
S. Abdelilah-Seyfried, Y.-M. Chan, C. Zeng, N. J. Justice, S. Younger-Shepherd, L. E. Sharp, S. Barbel, S. A. Meadows, L. Y. Jan, and Y. N. Jan A Gain-of-Function Screen for Genes That Affect the Development of the Drosophila Adult External Sensory Organ Genetics, June 1, 2000; 155(2): 733 - 752. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. S. Wesley and L. Saez Analysis of Notch Lacking the Carboxyl Terminus Identified in Drosophila Embryos J. Cell Biol., May 1, 2000; 149(3): 683 - 696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Massari and C. Murre Helix-Loop-Helix Proteins: Regulators of Transcription in Eucaryotic Organisms Mol. Cell. Biol., January 15, 2000; 20(2): 429 - 440. [Full Text]
|
|
|
|
|
|
E. Lai, R Bodner, and J. Posakony The enhancer of split complex of Drosophila includes four Notch-regulated members of the bearded gene family Development, January 8, 2000; 127(16): 3441 - 3455. [Abstract] [PDF]
|
|
|
|
|
|
A Baonza, J. de Celis, and A Garcia-Bellido Relationships between extramacrochaetae and Notch signalling in Drosophila wing development Development, January 6, 2000; 127(11): 2383 - 2393. [Abstract] [PDF]
|
|
|
|
|
|
C Schutt and R Nothiger Structure, function and evolution of sex-determining systems in Dipteran insects Development, January 2, 2000; 127(4): 667 - 677. [Abstract] [PDF]
|
|
|
|
|
|
E. Lai, R Bodner, J Kavaler, G Freschi, and J. Posakony Antagonism of notch signaling activity by members of a novel protein family encoded by the bearded and enhancer of split gene complexes Development, January 1, 2000; 127(2): 291 - 306. [Abstract] [PDF]
|
|
|
|
|
|
B. H. Jennings, D. M. Tyler, and S. J. Bray Target Specificities of Drosophila Enhancer of split Basic Helix-Loop-Helix Proteins Mol. Cell. Biol., July 1, 1999; 19(7): 4600 - 4610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Simpson, R Woehl, and K Usui The development and evolution of bristle patterns in Diptera Development, January 4, 1999; 126(7): 1349 - 1364. [Abstract] [PDF]
|
|
|
|
|
|
M Sato, T Kojima, T Michiue, and K Saigo Bar homeobox genes are latitudinal prepattern genes in the developing Drosophila notum whose expression is regulated by the concerted functions of decapentaplegic and wingless Development, January 4, 1999; 126(7): 1457 - 1466. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Katsube, M. Takahisa, R. Ueda, N. Hashimoto, M. Kobayashi, and S. Togashi Cortactin Associates with the Cell-Cell Junction Protein ZO-1 in both Drosophila and Mouse J. Biol. Chem., November 6, 1998; 273(45): 29672 - 29677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Carrera, S. Abrell, B. Kerber, U. Walldorf, A. Preiss, M. Hoch, and H. Jackle A modifier screen in the eye reveals control genes for Kruppel activity in the Drosophila embryo PNAS, September 1, 1998; 95(18): 10779 - 10784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Culí and J. Modolell Proneural gene self-stimulation in neural precursors: an essential mechanism for sense organ development that is regulated by Notch signaling Genes & Dev., July 1, 1998; 12(13): 2036 - 2047. [Abstract] [Full Text]
|
|
|
|
|
|
N. Brown, S Kanekar, M. Vetter, P. Tucker, D. Gemza, and T Glaser Math5 encodes a murine basic helix-loop-helix transcription factor expressed during early stages of retinal neurogenesis Development, January 12, 1998; 125(23): 4821 - 4833. [Abstract] [PDF]
|
|
|
|
|
|
Y Tomoyasu, M Nakamura, and N Ueno Role of dpp signalling in prepattern formation of the dorsocentral mechanosensory organ in Drosophila melanogaster Development, January 11, 1998; 125(21): 4215 - 4224. [Abstract] [PDF]
|
|
|
|
|
|
E. Lai, C Burks, and J. Posakony The K box, a conserved 3' UTR sequence motif, negatively regulates accumulation of enhancer of split complex transcripts Development, January 10, 1998; 125(20): 4077 - 4088. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Chao Zheng, R. Porat, P. Lu, and S. D. O' Neill PNZIP Is a Novel Mesophyll-Specific cDNA That Is Regulated by Phytochrome and a Circadian Rhythm and Encodes a Protein with a Leucine Zipper Motif Plant Physiology, January 1, 1998; 116(1): 27 - 35. [Abstract] [Full Text]
|
|
|
|
|
|
P. Alifragis, G. Poortinga, S. M. Parkhurst, and C. Delidakis A network of interacting transcriptional regulators involved in Drosophila neural fate specification revealed by the yeast two-hybrid system PNAS, November 25, 1997; 94(24): 13099 - 13104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cubadda, P. Heitzler, R. P. Ray, M. Bourouis, P. Ramain, W. Gelbart, P. Simpson, and M. Haenlin u-shaped encodes a zinc finger protein that regulates the proneural genes achaete and scute during the formation of bristles in Drosophila Genes & Dev., November 15, 1997; 11(22): 3083 - 3095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Anand, X. Yin, A. K. Shahidi, L. Grove, and E. V. Prochownik Novel Regulation of the Helix-Loop-Helix Protein Id1 by S5a, a Subunit of the 26 S Proteasome J. Biol. Chem., August 1, 1997; 272(31): 19140 - 19151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Giebel and J. A. Campos-Ortega Functional dissection of the Drosophila Enhancer of split protein, a suppressor of neurogenesis PNAS, June 10, 1997; 94(12): 6250 - 6254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Blader, N Fischer, G Gradwohl, F Guillemont, and U Strahle The activity of neurogenin1 is controlled by local cues in the zebrafish embryo Development, January 11, 1997; 124(22): 4557 - 4569. [Abstract] [PDF]
|
|
|
|
|
|
D Doherty, L. Jan, and Y. Jan The Drosophila neurogenic gene big brain, which encodes a membrane-associated protein, acts cell autonomously and can act synergistically with Notch and Delta Development, January 10, 1997; 124(19): 3881 - 3893. [Abstract] [PDF]
|
|
|
|
|
|
M Krause, M Park, J. Zhang, J Yuan, B Harfe, S. Xu, I Greenwald, M Cole, B Paterson, and A Fire A C. elegans E/Daughterless bHLH protein marks neuronal but not striated muscle development Development, January 6, 1997; 124(11): 2179 - 2189. [Abstract] [PDF]
|
|
|
|
|
|
J. B. Moss, E. N. Olson, and R. J. Schwartz The Myogenic Regulatory Factor MRF4 Represses the Cardiac alpha -Actin Promoter through a Negative-acting N-terminal Protein Domain J. Biol. Chem., December 6, 1996; 271(49): 31688 - 31694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Takahisa, S Togashi, T Suzuki, M Kobayashi, A Murayama, K Kondo, T Miyake, and R Ueda The Drosophila tamou gene, a component of the activating pathway of extramacrochaetae expression, encodes a protein homologous to mammalian cell-cell junction-associated protein ZO-1. Genes & Dev., July 15, 1996; 10(14): 1783 - 1795. [Abstract] [PDF]
|
|
|
|
|
|
N Papalopulu and C Kintner A posteriorising factor, retinoic acid, reveals that anteroposterior patterning controls the timing of neuronal differentiation in Xenopus neuroectoderm Development, January 11, 1996; 122(11): 3409 - 3418. [Abstract] [PDF]
|
|
|
|
|
|
P Heitzler, M Bourouis, L Ruel, C Carteret, and P Simpson Genes of the Enhancer of split and achaete-scute complexes are required for a regulatory loop between Notch and Delta during lateral signalling in Drosophila Development, January 1, 1996; 122(1): 161 - 171. [Abstract] [PDF]
|
|
|
|
|
|
A M Bailey and J W Posakony Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity. Genes & Dev., November 1, 1995; 9(21): 2609 - 2622. [Abstract] [PDF]
|
|
|
|
|
|
A Grens, E Mason, J. Marsh, and H. Bode Evolutionary conservation of a cell fate specification gene: the Hydra achaete-scute homolog has proneural activity in Drosophila Development, January 12, 1995; 121(12): 4027 - 4035. [Abstract] [PDF]
|
|
|
|
|
|
J. Treisman, Z. Lai, and G. Rubin Shortsighted acts in the decapentaplegic pathway in Drosophila eye development and has homology to a mouse TGF-beta-responsive gene Development, January 9, 1995; 121(9): 2835 - 2845. [Abstract] [PDF]
|
|
|
|
|
|
A Giangrande Proneural genes influence gliogenesis in Drosophila Development, January 2, 1995; 121(2): 429 - 438. [Abstract] [PDF]
|
|
|
|
|
|
M Van Doren, A M Bailey, J Esnayra, K Ede, and J W Posakony Negative regulation of proneural gene activity: hairy is a direct transcriptional repressor of achaete. Genes & Dev., November 15, 1994; 8(22): 2729 - 2742. [Abstract] [PDF]
|
|
|
|
|
|
S Ohsako, J Hyer, G Panganiban, I Oliver, and M Caudy Hairy function as a DNA-binding helix-loop-helix repressor of Drosophila sensory organ formation. Genes & Dev., November 15, 1994; 8(22): 2743 - 2755. [Abstract] [PDF]
|
|
|
|
|
|
P. Cubas, J. Modolell, and M. Ruiz-Gomez The helix-loop-helix extramacrochaetae protein is required for proper specification of many cell types in the Drosophila embryo Development, September 1, 1994; 120(9): 2555 - 2566. [Abstract] [PDF]
|
|
|
|
|
|
A Singson, M W Leviten, A G Bang, X H Hua, and J W Posakony Direct downstream targets of proneural activators in the imaginal disc include genes involved in lateral inhibitory signaling. Genes & Dev., September 1, 1994; 8(17): 2058 - 2071. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Parkhurst and P. Meneely Sex determination and dosage compensation: lessons from flies and worms Science, May 13, 1994; 264(5161): 924 - 932. [Abstract] [PDF]
|
|
|
|
|
|
C. Cabrera, M. Alonso, and H Huikeshoven Regulation of scute function by extramacrochaete in vitro and in vivo Development, January 12, 1994; 120(12): 3595 - 3603. [Abstract] [PDF]
|
|
|
|
|
|
B Oliver, J Singer, V Laget, G Pennetta, and D Pauli Function of Drosophila ovo+ in germ-line sex determination depends on X-chromosome number Development, January 11, 1994; 120(11): 3185 - 3195. [Abstract] [PDF]
|
|
|
|
|
|
H Vaessin, M Brand, L. Jan, and Y. Jan daughterless is essential for neuronal precursor differentiation but not for initiation of neuronal precursor formation in Drosophila embryo Development, January 4, 1994; 120(4): 935 - 945. [Abstract] [PDF]
|
|
|
|
|
|
C. Cummings and C Cronmiller The daughterless gene functions together with Notch and Delta in the control of ovarian follicle development in Drosophila Development, January 2, 1994; 120(2): 381 - 394. [Abstract] [PDF]
|
|
|
|
|
|
P. Ramain, P. Heitzler, M. Haenlin, and P. Simpson pannier, a negative regulator of achaete and scute in Drosophila, encodes a zinc finger protein with homology to the vertebrate transcription factor GATA-1 Development, December 1, 1993; 119(4): 1277 - 1291. [Abstract] [PDF]
|
|
|
|
|
|
J W Erickson and T W Cline A bZIP protein, sisterless-a, collaborates with bHLH transcription factors early in Drosophila development to determine sex. Genes & Dev., September 1, 1993; 7(9): 1688 - 1702. [Abstract] [PDF]
|
|
|
|
|
|
S Gonzalez-Crespo and M Levine Interactions between dorsal and helix-loop-helix proteins initiate the differentiation of the embryonic mesoderm and neuroectoderm in Drosophila. Genes & Dev., September 1, 1993; 7(9): 1703 - 1713. [Abstract] [PDF]
|
|
|
|
|
|
A Ghysen, C Dambly-Chaudiere, L Y Jan, and Y N Jan Cell interactions and gene interactions in peripheral neurogenesis. Genes & Dev., May 1, 1993; 7(5): 723 - 733. [PDF]
|
|
|
|
|
|
A. Jarman, M Brand, L. Jan, and Y. Jan The regulation and function of the helix-loop-helix gene, asense, in Drosophila neural precursors Development, January 9, 1993; 119(1): 19 - 29. [Abstract] [PDF]
|
|
|
|
|
|
R. Phillips and J. Whittle wingless expression mediates determination of peripheral nervous system elements in late stages of Drosophila wing disc development Development, January 6, 1993; 118(2): 427 - 438. [Abstract] [PDF]
|
|
|
|
|
|
S. Parkhurst, H. Lipshitz, and D Ish-Horowicz achaete-scute feminizing activities and Drosophila sex determination Development, January 2, 1993; 117(2): 737 - 749. [Abstract] [PDF]
|
|
|
|
|
|
M Steinmann-Zwicky Sex determination in Drosophila: sis-b, a major numerator element of the X:A ratio in the soma, does not contribute to the X:A ratio in the germ line Development, January 2, 1993; 117(2): 763 - 767. [Abstract] [PDF]
|
|
|
|
|
|
