|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 113, Issue 4 1069-1084, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
T Lallier and M Bronner-Fraser
Developmental Biology Center, University of California, Irvine 92717.
The mechanisms of neural crest cell interaction with laminin were explored using a quantitative cell attachment assay. With increasing substratum concentrations, an increasing percentage of neural crest cells adhere to laminin. Cell adhesion at all substratum concentrations was inhibited by the CSAT antibody, which recognizes the chick beta 1 subunit of integrin, suggesting that beta 1-integrins mediate neural crest cell interactions with laminin. The HNK-1 antibody, which recognizes a carbohydrate epitope, inhibited neural crest cell attachment to laminin at low coating concentrations (greater than 1 microgram ml-1; Low-LM), but not at high coating concentration of laminin (10 micrograms ml-1; High-LM). Attachment to Low-LM occurred in the absence of divalent cations, whereas attachment to High-LM required greater than 0.1 mM Ca2+ or Mn2+. Neural crest cell adherence to the E8 fragment of laminin, derived from its long arm, was similar to that on intact laminin at high and low coating concentrations, suggesting that this fragment contains the neural crest cell binding site(s). The HNK-1 antibody recognizes a protein of 165,000 Mr which is also found in immunoprecipitates using antibodies against the beta 1 subunit of integrin and is likely to be an integrin alpha subunit or an integrin-associated protein. Our results suggest that the HNK-1 epitope on neural crest cells is present on or associated with a novel or differentially glycosylated form of beta 1-integrin, which recognizes laminin in the apparent absence of divalent cations. We conclude that neural crest cells have at least two functionally independent means of attachment to laminin which are revealed at different substratum concentrations and/or conformations of laminin.
This article has been cited by other articles:
|
|
 |
|
  A. R. Morris, J. Drawbridge, and M. S. Steinberg Axolotl pronephric duct migration requires an epidermally derived, laminin 1-containing extracellular matrix and the integrin receptor {alpha}6{beta}1 Development, December 1, 2003; 130(23): 5601 - 5608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Freire, F. C. A. Gomes, R. Linden, V. M. Neto, and T. Coelho-Sampaio Structure of laminin substrate modulates cellular signaling for neuritogenesis J. Cell Sci., March 14, 2003; 115(24): 4867 - 4876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Gammill and M. Bronner-Fraser Genomic analysis of neural crest induction Development, March 14, 2003; 129(24): 5731 - 5741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Garwood, O. Schnadelbach, A. Clement, K. Schutte, A. Bach, and A. Faissner DSD-1-Proteoglycan Is the Mouse Homolog of Phosphacan and Displays Opposing Effects on Neurite Outgrowth Dependent on Neuronal Lineage J. Neurosci., May 15, 1999; 19(10): 3888 - 3899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Loike, L. Cao, S. Budhu, E. E. Marcantonio, J. E. Khoury, S. Hoffman, T. A. Yednock, and S. C. Silverstein Differential Regulation of beta 1 Integrins by Chemoattractants Regulates Neutrophil Migration through Fibrin J. Cell Biol., March 8, 1999; 144(5): 1047 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Sanderson and G. L. Smith Vaccinia Virus Induces Ca2+-Independent Cell-Matrix Adhesion during the Motile Phase of Infection J. Virol., December 1, 1998; 72(12): 9924 - 9933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Krull, A Collazo, S. Fraser, and M Bronner-Fraser Segmental migration of trunk neural crest: time-lapse analysis reveals a role for PNA-binding molecules Development, January 11, 1995; 121(11): 3733 - 3743. [Abstract] [PDF]
|
|
|
|
|
|
J. Evans, R. Schultz, and G. Kopf Mouse sperm-egg plasma membrane interactions: analysis of roles of egg integrins and the mouse sperm homologue of PH-30 (fertilin) beta J. Cell Sci., January 10, 1995; 108(10): 3267 - 3278. [Abstract] [PDF]
|
|
|
|
 |
|
 T Lallier and M Bronner-Fraser Inhibition of neural crest cell attachment by integrin antisense oligonucleotides Science, January 29, 1993; 259(5095): 692 - 695. [Abstract]
|
|
|
|
|
|
R Perris, J Syfrig, M Paulsson, and M Bronner-Fraser Molecular mechanisms of neural crest cell attachment and migration on types I and IV collagen J. Cell Sci., January 12, 1993; 106(4): 1357 - 1368. [Abstract] [PDF]
|
|
|
|
|
|
T Lallier, G Leblanc, K. Artinger, and M Bronner-Fraser Cranial and trunk neural crest cells use different mechanisms for attachment to extracellular matrices Development, January 11, 1992; 116(3): 531 - 541. [Abstract] [PDF]
|
|
