|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 113, Issue 4 1145-1158, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
N Papalopulu, JD Clarke, L Bradley, D Wilkinson, R Krumlauf and N Holder
MRC National Institute for Medical Research, Mill Hill, London, UK.
Retinoic acid is a very potent teratogen and has also been implicated as an endogenous developmental signalling molecule in vertebrate embryos. One of the regions of the embryo reliably affected by exogenously applied RA is the hindbrain. In this paper, we describe in detail the hindbrain of Xenopus laevis embryos briefly treated with various levels of RA at gastrula stages. Such treatments lead to development of embryos with loss of anterior structures. In addition, RA has a general effect on rhombomere morphology and specific effects on the development of the anterior rhombomeres. This effect is demonstrated using neurofilament antibodies, HRP staining and in situ hybridisation using a probe for expression of the Xenopus Krox-20 gene. Anatomically it is evident that the development of the hindbrain normally anterior to the otocyst (rhombomeres 1-4) is abnormal following RA treatment. Sensory and motor axons of cranial nerves V and VII form a single root and the peripheral paths of V and VII and IX and X are also abnormal, as is the more anterior location of the otocyst. These anatomical changes are accompanied by changes in the pattern of expression for the gene XKrox-20, which normally expresses in rhombomeres 3 and 5, but is found in a single band in the anterior hindbrain of treated embryos which standardly fail to generate the normal external segmental appearance. The results are discussed in terms of both the teratogenic and possible endogenous roles of RA during normal development of the central nervous system. We conclude that low doses of RA applied during gastrulation have specific effects on the anterior Xenopus hindbrain which appear to be evolutionarily conserved in the light of similar recent findings in zebrafish.
This article has been cited by other articles:
|
|
 |
|
  H. Chen, H. Zhang, J. Lee, X. Liang, X. Wu, T. Zhu, P.-k. Lo, X. Zhang, and S. Sukumar HOXA5 Acts Directly Downstream of Retinoic Acid Receptor {beta} and Contributes to Retinoic Acid Induced Apoptosis and Growth Inhibition Cancer Res., September 1, 2007; 67(17): 8007 - 8013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rastegar, L. Kobrossy, E. N. Kovacs, I. Rambaldi, and M. Featherstone Sequential Histone Modifications at Hoxd4 Regulatory Regions Distinguish Anterior from Posterior Embryonic Compartments Mol. Cell. Biol., September 15, 2004; 24(18): 8090 - 8103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Murakami, M. Pasqualetti, Y. Takio, S. Hirano, F. M. Rijli, and S. Kuratani Segmental development of reticulospinal and branchiomotor neurons in lamprey: insights into the evolution of the vertebrate hindbrain Development, March 1, 2004; 131(5): 983 - 995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-K. Bae, T. Shimizu, T. Yabe, C.-H. Kim, T. Hirata, H. Nojima, O. Muraoka, T. Hirano, and M. Hibi A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish Development, May 1, 2003; 130(9): 1853 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Foley, I Skromne, and C. Stern Reconciling different models of forebrain induction and patterning: a dual role for the hypoblast Development, January 9, 2000; 127(17): 3839 - 3854. [Abstract] [PDF]
|
|
|
|
 |
|
 A. R. Uzgare, J. A. Uzman, H. M. El-Hodiri, and A. K. Sater Mitogen-activated protein kinase and neural specification in Xenopus PNAS, December 8, 1998; 95(25): 14833 - 14838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Huang, S. Chen, A. Langston, and L. Gudas A conserved retinoic acid responsive element in the murine Hoxb-1 gene is required for expression in the developing gut Development, January 8, 1998; 125(16): 3235 - 3246. [Abstract] [PDF]
|
|
|
|
|
|
M Studer, A Gavalas, H Marshall, L Ariza-McNaughton, F. Rijli, P Chambon, and R Krumlauf Genetic interactions between Hoxa1 and Hoxb1 reveal new roles in regulation of early hindbrain patterning Development, January 3, 1998; 125(6): 1025 - 1036. [Abstract] [PDF]
|
|
|
|
|
|
J van der Wees, J. Schilthuis, C. Koster, H Diesveld-Schipper, G. Folkers, P. van der Saag, M. Dawson, K Shudo, B van der Burg, and A. Durston Inhibition of retinoic acid receptor-mediated signalling alters positional identity in the developing hindbrain Development, January 2, 1998; 125(3): 545 - 556. [Abstract] [PDF]
|
|
|
|
 |
|
 A. W. Langston, J. R. Thompson, and L. J. Gudas Retinoic Acid-responsive Enhancers Located 3' of the Hox A and Hox B Homeobox Gene Clusters. FUNCTIONAL ANALYSIS J. Biol. Chem., January 24, 1997; 272(4): 2167 - 2175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Nittenberg, K Patel, Y Joshi, R Krumlauf, D. Wilkinson, P. Brickell, C Tickle, and J. Clarke Cell movements, neuronal organisation and gene expression in hindbrains lacking morphological boundaries Development, January 6, 1997; 124(12): 2297 - 2306. [Abstract] [PDF]
|
|
|
|
|
|
H. Lu, G Eichele, and C Thaller Ligand-bound RXR can mediate retinoid signal transduction during embryogenesis Development, January 1, 1997; 124(1): 195 - 203. [Abstract] [PDF]
|
|
|
|
|
|
B Blumberg, J Bolado, T. Moreno, C Kintner, R. Evans, and N Papalopulu An essential role for retinoid signaling in anteroposterior neural patterning Development, January 1, 1997; 124(2): 373 - 379. [Abstract] [PDF]
|
|
|
|
|
|
C. Sharpe and K Goldstone Retinoid receptors promote primary neurogenesis in Xenopus Development, January 1, 1997; 124(2): 515 - 523. [Abstract] [PDF]
|
|
|
|
|
|
K. Kroll and E Amaya Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation Development, January 10, 1996; 122(10): 3173 - 3183. [Abstract] [PDF]
|
|
|
|
|
|
D Alexandre, J. Clarke, E Oxtoby, Y. Yan, T Jowett, and N Holder Ectopic expression of Hoxa-1 in the zebrafish alters the fate of the mandibular arch neural crest and phenocopies a retinoic acid-induced phenotype Development, January 3, 1996; 122(3): 735 - 746. [Abstract] [PDF]
|
|
|
|
|
|
E Gale, V Prince, A Lumsden, J Clarke, N Holder, and M Maden Late effects of retinoic acid on neural crest and aspects of rhombomere Development, January 3, 1996; 122(3): 783 - 793. [Abstract] [PDF]
|
|
|
|
|
|
Y Katsuyama, S Wada, S Yasugi, and H Saiga Expression of the labial group Hox gene HrHox-1 and its alteration induced by retinoic acid in development of the ascidian Halocynthia roretzi Development, January 10, 1995; 121(10): 3197 - 3205. [Abstract] [PDF]
|
|
|
|
|
|
I. Blitz and K. Cho Anterior neurectoderm is progressively induced during gastrulation: the role of the Xenopus homeobox gene orthodenticle Development, January 4, 1995; 121(4): 993 - 1004. [Abstract] [PDF]
|
|
|
|
|
|
Y. Lee, N Osumi-Yamashita, Y Ninomiya, C. Moon, U Eriksson, and K Eto Retinoic acid stage-dependently alters the migration pattern and identity of hindbrain neural crest cells Development, January 3, 1995; 121(3): 825 - 837. [Abstract] [PDF]
|
|
|
|
|
|
M Taira, H Otani, M Jamrich, and I. Dawid Expression of the LIM class homeobox gene Xlim-1 in pronephros and CNS cell lineages of Xenopus embryos is affected by retinoic acid and exogastrulation Development, January 6, 1994; 120(6): 1525 - 1536. [Abstract] [PDF]
|
|
|
|
|
|
E. Dekker, M. Vaessen, C van den Berg, A Timmermans, S Godsave, T Holling, P Nieuwkoop, A Geurts van Kessel, and A Durston Overexpression of a cellular retinoic acid binding protein (xCRABP) causes anteroposterior defects in developing Xenopus embryos Development, January 4, 1994; 120(4): 973 - 985. [Abstract] [PDF]
|
|
|
|
|
|
M. W. Kelley, X. M. Xu, M. A. Wagner, M. E. Warchol, and J. T. Corwin The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture Development, December 1, 1993; 119(4): 1041 - 1053. [Abstract] [PDF]
|
|
|
|
 |
|
 P J Swiatek and T Gridley Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox20. Genes & Dev., November 1, 1993; 7(11): 2071 - 2084. [Abstract] [PDF]
|
|
|
|
|
|
L Jeannotte, M Lemieux, J Charron, F Poirier, and E J Robertson Specification of axial identity in the mouse: role of the Hoxa-5 (Hox1.3) gene. Genes & Dev., November 1, 1993; 7(11): 2085 - 2096. [Abstract] [PDF]
|
|
|
|
|
|
T. Schuh, B. Hall, J. Kraft, M. Privalsky, and D Kimelman v-erbA and citral reduce the teratogenic effects of all-trans retinoic acid and retinol, respectively, in Xenopus embryogenesis Development, January 11, 1993; 119(3): 785 - 798. [Abstract] [PDF]
|
|
|
|
|
|
M. Saha, R. Michel, K. Gulding, and R. Grainger A Xenopus homebox gene defines dorsal-ventral domains in the developing brain Development, January 5, 1993; 118(1): 193 - 202. [Abstract] [PDF]
|
|
|
|
|
|
E Ruberte, V Friederich, P Chambon, and G Morriss-Kay Retinoic acid receptors and cellular retinoid binding proteins. III. Their differential transcript distribution during mouse nervous system development Development, January 5, 1993; 118(1): 267 - 282. [Abstract] [PDF]
|
|
|
|
|
|
M. Frohman, G. Martin, S. Cordes, L. Halamek, and G. Barsh Altered rhombomere-specific gene expression and hyoid bone differentiation in the mouse segmentation mutant, kreisler (kr) Development, January 3, 1993; 117(3): 925 - 936. [Abstract] [PDF]
|
|
|
|
|
|
A. Zimmer and A. Zimmer Induction of a RAR beta 2-lacZ transgene by retinoic acid reflects the neuromeric organization of the central nervous system Development, December 1, 1992; 116(4): 977 - 983. [Abstract] [PDF]
|
|
|
|
 |
|
 T Doniach, C. Phillips, and J. Gerhart Planar induction of anteroposterior pattern in the developing central nervous system of Xenopus laevis Science, July 24, 1992; 257(5069): 542 - 545. [Abstract] [PDF]
|
|
|
|
|
|
F. Grun, Y. Hirose, S. Kawauchi, T. Ogura, and K. Umesono Aldehyde Dehydrogenase 6, a Cytosolic Retinaldehyde Dehydrogenase Prominently Expressed in Sensory Neuroepithelia during Development J. Biol. Chem., December 22, 2000; 275(52): 41210 - 41218. [Abstract] [Full Text] [PDF]
|
|
