Development, Vol 114, Issue 2 507-519, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

Disruption of pioneer growth cone guidance in vivo by removal of glycosyl-phosphatidylinositol-anchored cell surface proteins

WS Chang, K Serikawa, K Allen and D Bentley
Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Cell surface proteins anchored to membranes via covalently attached glycosyl-phosphatidylinositol (GPI) have been implicated in neuronal adhesion, promotion of neurite outgrowth and directed cell migration. Treatment of grasshopper embryos with bacterial phosphatidylinositol-specific phospholipase C (PI-PLC), an enzyme that cleaves the GPI anchor, often induced disruptions in the highly stereotyped migrations of peripheral pioneer growth cones and afferent neuron cell bodies. In distal limb regions of embryos treated with PI-PLC at early stages of pioneer axon outgrowth, growth cones lost their proximal orientation toward the central nervous system (CNS) and turned distally. Pioneer growth cones in treated limbs also failed to make a characteristic ventral turn along the trochanter-coxa (Tr-Cx) segment boundary, and instead continued to grow proximally across the boundary. Treatment at an earlier stage of development caused pre-axonogenesis Cx1 neurons to abandon their normal circumferential migration and reorient toward the CNS. None of these abnormal phenotypes were observed in limbs of untreated embryos or embryos exposed to other phospholipases that do not release GPI-anchored proteins. Incubation of embryos with PI-PLC effectively removed immunoreactivity for fasciclin I, a GPI-anchored protein expressed on a subset of neuronal surfaces. These results suggest that cell surface GPI-anchored proteins are involved in pioneer growth cone guidance and in pre-axonogenesis migration of neurons in the grasshopper limb bud in vivo.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. Wright, M. Snyder, K. Schwinof, S Combes, and P. Copenhaver A role for fasciclin II in the guidance of neuronal migration Development, January 6, 1999; 126(14): 3217 - 3228. [Abstract] [PDF]

				E. Seaver, E. Carpenter, and M. Bastiani REGA-1 is a GPI-linked member of the immunoglobulin superfamily present on restricted regions of sheath cell processes in grasshopper Development, January 2, 1996; 122(2): 567 - 578. [Abstract] [PDF]

				G. MacGregor, B. Zambrowicz, and P Soriano Tissue non-specific alkaline phosphatase is expressed in both embryonic and extraembryonic lineages during mouse embryogenesis but is not required for migration of primordial germ cells Development, January 5, 1995; 121(5): 1487 - 1496. [Abstract] [PDF]

				V Zhukareva and P Levitt The limbic system-associated membrane protein (LAMP) selectively mediates interactions with specific central neuron populations Development, January 4, 1995; 121(4): 1161 - 1172. [Abstract] [PDF]

				M. Ganfornina, D Sanchez, and M. Bastiani Lazarillo, a new GPI-linked surface lipocalin, is restricted to a subset of neurons in the grasshopper embryo Development, January 1, 1995; 121(1): 123 - 134. [Abstract] [PDF]

				P. McGuire and S. Alexander Inhibition of urokinase synthesis and cell surface binding alters the motile behavior of embryonic endocardial-derived mesenchymal cells in vitro Development, January 7, 1993; 118(3): 931 - 939. [Abstract] [PDF]

				R. Karlstrom, L. Wilder, and M. Bastiani Lachesin: an immunoglobulin superfamily protein whose expression correlates with neurogenesis in grasshopper embryos Development, January 6, 1993; 118(2): 509 - 522. [Abstract] [PDF]

				W. Chang, K. Zachow, and D Bentley Expression of epithelial alkaline phosphatase in segmentally iterated bands during grasshopper limb morphogenesis Development, January 6, 1993; 118(2): 651 - 663. [Abstract] [PDF]