|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 115, Issue 1 305-312, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
M Aghayan, LV Rao, RM Smith, L Jarett, MJ Charron, B Thorens and S Heyner
Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, Philadelphia 19104.
Two general mechanisms mediate glucose transport, one is a sodium-coupled glucose transporter found in the apical border of intestinal and kidney epithelia, while the other is a sodium-independent transport system. Of the latter, several facilitated transporters have been identified, including GLUT1 (erythrocyte/brain), GLUT2 (liver) and GLUT4 (adipose/muscle) isoforms. In this study, we used Western-blot analysis and high resolution immunoelectron microscopy (IEM) to investigate the stage-related expression and cellular localization of GLUT1, 2 and 4. The Western blot results demonstrate that GLUT1 is detectable in the oocyte and throughout preimplantation development. GLUT2 isoforms were not detectable until the blastocyst stage, while the GLUT4 isoform was undetectable in the oocyte through blastocyst stages. The present findings confirm previous studies at the molecular level which demonstrated that mRNAs encoding the same GLUT isoforms are detectable at corresponding developmental stages. GLUT1 and GLUT2 display different cellular distributions at the blastocyst stage as shown by IEM studies. GLUT1 has a widespread distribution in both trophectoderm and inner cell mass cells, while GLUT2 is located on trophectoderm membranes facing the blastocyst cavity. This observation suggests a different functional significance for these isoforms during mouse preimplantation development.
This article has been cited by other articles:
|
|
 |
|
  A. Ganguly, R. A. McKnight, S. Raychaudhuri, B.-C. Shin, Z. Ma, K. Moley, and S. U. Devaskar Glucose transporter isoform-3 mutations cause early pregnancy loss and fetal growth restriction Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1241 - E1255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K Riley and K. H Moley Glucose utilization and the PI3-K pathway: mechanisms for cell survival in preimplantation embryos. Reproduction, May 1, 2006; 131(5): 823 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wang, J. M. Pascual, H. Yang, K. Engelstad, X. Mao, J. Cheng, J. Yoo, J. L. Noebels, and D. C. De Vivo A mouse model for Glut-1 haploinsufficiency Hum. Mol. Genet., April 1, 2006; 15(7): 1169 - 1179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. D Houghton Role of gap junctions during early embryo development Reproduction, February 1, 2005; 129(2): 129 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Navarrete Santos, S. Tonack, M. Kirstein, S. Kietz, and B. Fischer Two insulin-responsive glucose transporter isoforms and the insulin receptor are developmentally expressed in rabbit preimplantation embryos Reproduction, November 1, 2004; 128(5): 503 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Navarrete Santos, S. Tonack, M. Kirstein, M. Pantaleon, P. Kaye, and B. Fischer Insulin acts via mitogen-activated protein kinase phosphorylation in rabbit blastocysts Reproduction, November 1, 2004; 128(5): 517 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Riley, J. M. Heeley, A. H. Wyman, E. L. Schlichting, and K. H. Moley TRAIL and KILLER Are Expressed and Induce Apoptosis in the Murine Preimplantation Embryo Biol Reprod, September 1, 2004; 71(3): 871 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. O. Carayannopoulos, A. Schlein, A. Wyman, M. Chi, C. Keembiyehetty, and K. H. Moley GLUT9 Is Differentially Expressed and Targeted in the Preimplantation Embryo Endocrinology, March 1, 2004; 145(3): 1435 - 1443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Heilig, T. Saunders, F. C. Brosius III, K. Moley, K. Heilig, R. Baggs, L. Guo, and D. Conner Glucose transporter-1-deficient mice exhibit impaired development and deformities that are similar to diabetic embryopathy PNAS, December 23, 2003; 100(26): 15613 - 15618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Heilig, F. Brosius, B. Siu, L. Concepcion, R. Mortensen, K. Heilig, M. Zhu, R. Weldon, G. Wu, and D. Conner Implications of Glucose Transporter Protein Type 1 (GLUT1)-Haplodeficiency in Embryonic Stem Cells for Their Survival in Response to Hypoxic Stress Am. J. Pathol., November 1, 2003; 163(5): 1873 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Wyman, M. Chi, J. Riley, M. O. Carayannopoulos, C. Yang, K. J. Coker, J. E. Pessin, and K. H. Moley Syntaxin 4 Expression Affects Glucose Transporter 8 Translocation and Embryo Survival Mol. Endocrinol., October 1, 2003; 17(10): 2096 - 2102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. McAvey, G. B. Wortzman, C. J. Williams, and J. P. Evans Involvement of Calcium Signaling and the Actin Cytoskeleton in the Membrane Block to Polyspermy in Mouse Eggs Biol Reprod, October 1, 2002; 67(4): 1342 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Pinto, M. O. Carayannopoulos, A. Hoehn, L. Dowd, and K. H. Moley Glucose Transporter 8 Expression and Translocation Are Critical for Murine Blastocyst Survival Biol Reprod, June 1, 2002; 66(6): 1729 - 1733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Turkay Korgun, R. Demir, A. Hammer, G. Dohr, G. Desoye, G. Skofitsch, and T. Hahn Glucose Transporter Expression in Rat Embryo and Uterus During Decidualization, Implantation, and Early Postimplantation Biol Reprod, November 1, 2001; 65(5): 1364 - 1370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Leppens-Luisier, F. Urner, and D. Sakkas Facilitated glucose transporters play a crucial role throughout mouse preimplantation embryo development Hum. Reprod., June 1, 2001; 16(6): 1229 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Ludwig, M. Lane, and B. D. Bavister Differential Effect of Hexoses on Hamster Embryo Development in Culture Biol Reprod, May 1, 2001; 64(5): 1366 - 1374. [Abstract] [Full Text]
|
|
|
|
|
|
M. Pantaleon, J. P. Ryan, M. Gil, and P. L. Kaye An Unusual Subcellular Localization of GLUT1 and Link with Metabolism in Oocytes and Preimplantation Mouse Embryos Biol Reprod, April 1, 2001; 64(4): 1247 - 1254. [Abstract] [Full Text]
|
|
|
|
|
|
M. O. Carayannopoulos, M. M.-Y. Chi, Y. Cui, J. M. Pingsterhaus, R. A. McKnight, M. Mueckler, S. U. Devaskar, and K. H. Moley GLUT8 is a glucose transporter responsible for insulin-stimulated glucose uptake in the blastocyst PNAS, June 20, 2000; 97(13): 7313 - 7318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-l. Wang, N. Chinookoswong, S. Scully, M. Qi, and Z.-Q. Shi Differential Effects of Leptin in Regulation of Tissue Glucose Utilization in Vivo Endocrinology, May 1, 1999; 140(5): 2117 - 2124. [Abstract] [Full Text]
|
|
|
|
|
|
F. Urner and D. Sakkas A Possible Role for the Pentose Phosphate Pathway of Spermatozoa inGamete Fusion in the Mouse Biol Reprod, March 1, 1999; 60(3): 733 - 739. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Hahn, S. Barth, U. Weiss, W. Mosgoeller, and G. Desoye Sustained hyperglycemia in vitro down-regulates the GLUT1 glucose transport system of cultured human term placental trophoblast: a mechanism to protect fetal development? FASEB J, September 1, 1998; 12(12): 1221 - 1231. [Abstract] [Full Text]
|
|
|
|
|
|
K. H. Moley, M. M.-Y. Chi, and M. M. Mueckler Maternal hyperglycemia alters glucose transport and utilization in mouse preimplantation embryos Am J Physiol Endocrinol Metab, July 1, 1998; 275(1): E38 - E47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pantaleon, E. J. Whiteside, M. B. Harvey, R. T. Barnard, M. J. Waters, and P. L. Kaye Functional growth hormone (GH) receptors and GH are expressed by preimplantation mouse embryos: A role for GH in early embryogenesis? PNAS, May 13, 1997; 94(10): 5125 - 5130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pantaleon, M. B. Harvey, W. S. Pascoe, D. E. James, and P. L. Kaye Glucose transporter GLUT3: Ontogeny, targeting, and role in the mouse blastocyst PNAS, April 15, 1997; 94(8): 3795 - 3800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Smith and T Gridley Differential screening of a PCR-generated mouse embryo cDNA library: glucose transporters are differentially expressed in early postimplantation mouse embryos Development, January 11, 1992; 116(3): 555 - 561. [Abstract] [PDF]
|
|
