|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 115, Issue 2 421-426, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
TA Bayer and JA Campos-Ortega
Institut fur Entwicklungsbiologie, Universitat zu Koln, Federal Republic of Germany.
In order to screen for developmentally active chromosomal domains during zebrafish embryogenesis, we generated transgenic fish by microinjecting two different lacZ reporter constructs into fertilized eggs. Transgenic fish were screened among the progeny of injected fish (F0) crossed to non-injected fish. Groups of 15 to 20 progeny of each cross were tested for lacZ expression and/or transmission of injected sequences using PCR and Southern hybridizations. Progeny from 2 of 102 fish injected with supercoiled constructs containing Rous sarcoma virus promoter sequences showed apparently spatially regulated beta-galactosidase (beta-Gal) activity. However, we were not able to detect this reporter construct in DNA from fins of F1 fish. Injections of a linear reporter construct containing mouse heat-shock promoter sequences revealed transmission of injected sequences to F1 progeny in about 6% of cases (8 of 129 fish, tested with PCR). We found one lacZ-expressing line that showed a spatially and temporally restricted expression of lacZ and, therefore, features typical characteristics of "enhancer trap" lines. In this line, lacZ expression starts at 16 hours post-fertilization in trigeminal ganglion cells. At about 24 hours lacZ expression can be detected in trigeminal ganglion neurons and Rohon-Beard neurons, indicating that the development of these two cell types shows common features. The reporter gene has integrated as a single copy. The founder fish was mosaic: 19% of its offspring (3 of 16 tested animals) carried the reporter construct in their fins; about 51% (13 of 27 tested animals) of the progeny of F1 fish were beta-Gal positive indicating full hemizygosity.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  S. Ellingsen, M. A. Laplante, M. Konig, H. Kikuta, T. Furmanek, E. A. Hoivik, and T. S. Becker Large-scale enhancer detection in the zebrafish genome Development, September 1, 2005; 132(17): 3799 - 3811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. T. DOW and S. A. DAVIES Integrative Physiology and Functional Genomics of Epithelial Function in a Genetic Model Organism Physiol Rev, July 1, 2003; 83(3): 687 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Morin, R. Daneman, M. Zavortink, and W. Chia A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila PNAS, December 6, 2001; (2001) 261408198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Smith and K. C. Gross A Family of at Least Seven beta -Galactosidase Genes Is Expressed during Tomato Fruit Development Plant Physiology, July 1, 2000; 123(3): 1173 - 1184. [Abstract] [Full Text]
|
|
|
|
|
|
M. Halloran, M Sato-Maeda, J. Warren, F Su, Z Lele, P. Krone, J. Kuwada, and W Shoji Laser-induced gene expression in specific cells of transgenic zebrafish Development, January 5, 2000; 127(9): 1953 - 1960. [Abstract] [PDF]
|
|
|
|
|
|
J. M. Fadool, D. L. Hartl, and J. E. Dowling Transposition of the mariner element from Drosophila mauritiana in zebrafish PNAS, April 28, 1998; 95(9): 5182 - 5186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Noll, M. Sturtevant, R. Gollapudi, and E Bier New functions of the Drosophila rhomboid gene during embryonic and adult development are revealed by a novel genetic method, enhancer piracy Development, January 8, 1994; 120(8): 2329 - 2338. [Abstract] [PDF]
|
|
|
|
|
|
X. Morin, R. Daneman, M. Zavortink, and W. Chia A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila PNAS, December 18, 2001; 98(26): 15050 - 15055. [Abstract] [Full Text] [PDF]
|
|
