|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 115, Issue 4 1133-1147, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
R Palacios and S Nishikawa
Basel Institute for Immunology, Switzerland.
We have used a c-kit-specific monoclonal antibody, immuno-fluorescence staining and flow fluorocytometry or microscopy analysis to assess the cell surface expression of the c-kit receptor on a panel of non-transformed clones representing different stages of T- and B-lymphocyte development, freshly isolated lymphoid cells from thymus, bone marrow and spleen of young adult C57BL/6 mice and cells from yolk sac, thymus and liver of developing C57BL/6 mouse embryos. Pro-T, Pro-B and Pre-B clones derived from thymus or liver of 14-day embryos are c-kit+. Starting at day 8 to 8.5 in yolk sac, day-10 in fetal liver, and day 11 to 12 in fetal thymus, there are many c-kit+ cells. The number of c-kit+ cells in liver and thymus increases up to day 15 and progressively decreases thereafter. Cell sorter purified c-kit+ day 14 fetal liver cells fully reconstitute the T and B cell compartments of immunodeficient Scid mice. Stromal cells or epithelial cells derived from fetal thymus or liver, which can support growth and differentiation of c-kit+ lymphocyte progenitor clones, synthesize mRNA for Steel Factor (SF), the ligand of c-kit. In the adult mouse, however, c-kit expression is restricted to very early stages of T- and B-lymphocyte development (multipotent progenitors, B-cell/myelocytic progenitors, Pro-T and Pro-B lymphocyte progenitors). Most cells at the Pre-T, Pre-B and later stages of development do not bear detectable c-kit. Using Cos-1 cells transfected with mouse SF-cDNA and an antagonistic c-kit receptor-specific antibody, we show that the c-kit/SF system contributes to the survival of lymphocyte progenitors and enhances the proliferative responses of these cells to other growth factors (i.e. IL2, IL3, IL4, IL7). However, the c-kit receptor/SF ligand pair is neither sufficient nor necessary for the differentiation of lymphocyte progenitors into mature T- or B-lymphocytes. Finally, in stromal cell lines from fetal liver and adult bone marrow and thymic epithelial cell lines the level of steady state SF-RNA transcripts is inversely correlated with that of IL-7-mRNA. Moreover, IL7 inhibits the synthesis of SF-mRNA in stromal cells and rIL6 abrogates this inhibitory effect of rIL7. Thus, the expression of SF in stromal cells is subjected to complex regulation by other cytokines produced by the same stromal cells or by neighboring cells in a given microenvironment.(ABSTRACT TRUNCATED AT 400 WORDS)
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  W.-L. Li, Y. Yamada, M. Ueno, S. Nishikawa, S.-I. Nishikawa, and N. Takakura Platelet Derived Growth Factor Receptor Alpha Is Essential for Establishing a Microenvironment That Supports Definitive Erythropoiesis J. Biochem., August 1, 2006; 140(2): 267 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Goldman, L. K. Berg, M. C. Heinrich, and J. L. Christian Ectodermally derived steel/stem cell factor functions non-cell autonomously during primitive erythropoiesis in Xenopus Blood, April 15, 2006; 107(8): 3114 - 3121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-m. Su and N. R. Manley Hoxa3 and Pax1 Transcription Factors Regulate the Ability of Fetal Thymic Epithelial Cells to Promote Thymocyte Development J. Immunol., June 1, 2000; 164(11): 5753 - 5760. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Krosl, G. He, M. Lefrancois, F. Charron, P.-H. Romeo, P. Jolicoeur, I. R. Kirsch, M. Nemer, and T. Hoang Transcription Factor SCL Is Required for c-kit Expression and c-Kit Function in Hemopoietic Cells J. Exp. Med., August 3, 1998; 188(3): 439 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. C. Broudy Stem Cell Factor and Hematopoiesis Blood, August 15, 1997; 90(4): 1345 - 1364. [Full Text] [PDF]
|
|
|
|
|
|
C. L. Miller, V. I. Rebel, C. D. Helgason, P. M. Lansdorp, and C. J. Eaves Impaired Steel Factor Responsiveness Differentially Affects the Detection and Long-Term Maintenance of Fetal Liver Hematopoietic Stem Cells In Vivo Blood, February 15, 1997; 89(4): 1214 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yasuda, I. Nishijima, S. Watanabe, K.-i. Arai, A. Zlotnik, and T. A. Moore Human Granulocyte-Macrophage Colony-Stimulating Factor (hGM-CSF) Induces Inhibition of Intrathymic T-Cell Development in hGM-CSF Receptor Transgenic Mice Blood, February 15, 1997; 89(4): 1349 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Ryan, B. L. Nuccie, I. Ritterman, J. L. Liesveld, C. N. Abboud, and R. A. Insel Expression of Interleukin-7 Receptor by Lineage-Negative Human Bone Marrow Progenitors With Enhanced Lymphoid Proliferative Potential and B-Lineage Differentiation Capacity Blood, February 1, 1997; 89(3): 929 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Wallin, H Eibel, A Neubuser, J Wilting, H Koseki, and R Balling Pax1 is expressed during development of the thymus epithelium and is required for normal T-cell maturation Development, January 1, 1996; 122(1): 23 - 30. [Abstract] [PDF]
|
|
|
|
 |
|
 F Young, B Ardman, Y Shinkai, R Lansford, T K Blackwell, M Mendelsohn, A Rolink, F Melchers, and F W Alt Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation. Genes & Dev., May 1, 1994; 8(9): 1043 - 1057. [Abstract] [PDF]
|
|
|
|
|
|
M Ogawa, S Nishikawa, K Yoshinaga, S Hayashi, T Kunisada, J Nakao, T Kina, T Sudo, H Kodama, and S Nishikawa Expression and function of c-Kit in fetal hemopoietic progenitor cells: transition from the early c-Kit-independent to the late c-Kit-dependent wave of hemopoiesis in the murine embryo Development, January 3, 1993; 117(3): 1089 - 1098. [Abstract] [PDF]
|
|
