|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 116, Issue 1 95-102, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
EM Jagerbauer, A Fraser, EW Herbst, R Kothary and R Fundele
Institut fur Biologie III, Universitat Freiburg, Germany.
The ability of parthenogenetic (pg) cells to contribute to proliferating stem cell populations of postnatal aggregation chimeras was investigated. Using DNA in situ analysis, pg participation was observed in highly regenerative epithelia of various regions of the gastrointestinal tract, e.g., stomach, duodenum and colon, in the epithelia of tongue and uterus and in the epidermis. Pg cells also contributed to the epithelium of the urinary bladder, which is characterized by a relatively slow cellular turnover. Using a sensitive proliferation marker to determine division rate of pg and normal (wt) cells in tissues of a 24-day-old chimera, no significant differences between pg and fertilized cells were observed. However, in colon and uterus of a pg <==> wt chimera aged 101 days, a significant loss of proliferative capacity of pg cells was found. In the colon, this loss of proliferative potential was accompanied by an altered morphology of pg crypts. In general, they were situated at the periphery of the epithelium and lacked access to the lumen, with consequent cystic enlargement and flattened epithelium. No obvious morphological changes were observed in the pg-derived areas of the uterine epithelium of this chimera. Our results provide evidence that pg cells can persist as proliferating stem cells in various tissues of early postnatal chimeras. They suggest that pg-derived stem cells may cease to proliferate in restricted areas of the gastrointestinal tract and in the uterine epithelium of pg <==> wt chimeras of advanced age.(ABSTRACT TRUNCATED AT 250 WORDS)
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Eckardt, N. A. Leu, H. L. Bradley, H. Kato, K. D. Bunting, and K. J. McLaughlin Hematopoietic reconstitution with androgenetic and gynogenetic stem cells Genes & Dev., February 15, 2007; 21(4): 409 - 419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Nakabayashi, S Makino, S Minagawa, A C Smith, J S Bamforth, P Stanier, M Preece, L Parker-Katiraee, T Paton, M Oshimura, et al. Genomic imprinting of PPP1R9A encoding neurabin I in skeletal muscle and extra-embryonic tissues J. Med. Genet., August 1, 2004; 41(8): 601 - 608. [Full Text] [PDF]
|
|
|
|
 |
|
 N. Allen, S. Barton, K Hilton, M. Norris, and M. Surani A functional analysis of imprinting in parthenogenetic embryonic stem cells Development, January 6, 1994; 120(6): 1473 - 1482. [Abstract] [PDF]
|
|
