Development, Vol 116, Issue 3 585-600, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

Expression of alpha 1 integrin, a laminin-collagen receptor, during myogenesis and neurogenesis in the avian embryo

JL Duband, AM Belkin, J Syfrig, JP Thiery and VE Koteliansky
Institut Jacques Monod, Universite Paris, France.

In this study, we have examined the spatiotemporal distribution of the alpha 1 integrin subunit, a putative laminin and collagen receptor, in avian embryos, using immunofluorescence microscopy and immunoblotting techniques. We used an antibody raised against a gizzard 175 x 10(3) M(r) membrane protein which was described previously and which we found to be immunologically identical to the chicken alpha 1 integrin subunit. In adult avian tissues, alpha 1 integrin exhibited a very restricted pattern of expression; it was detected only in smooth muscle and in capillary endothelial cells. In the developing embryo, alpha 1 integrin subunit expression was discovered in addition to smooth muscle and capillary endothelial cells, transiently, in both central and peripheral nervous systems and in striated muscles, in association with laminin and collagen IV. alpha 1 integrin was practically absent from most epithelial tissues, including the liver, pancreas and kidney tubules, and was weakly expressed by tissues that were not associated with laminin and collagen IV. In the nervous system, alpha 1 integrin subunit expression occurred predominantly at the time of early neuronal differentiation. During skeletal muscle development, alpha 1 integrin was expressed on myogenic precursors, during myoblast migration, and in differentiating myotubes. alpha 1 integrin disappeared from skeletal muscle cells as they became contractile. In visceral and vascular smooth muscles, alpha 1 integrin appeared specifically during early smooth muscle cell differentiation and, later, was permanently expressed after cell maturation. These results indicate that (i) the expression pattern of alpha 1 integrin is consistent with a function as a laminin/collagen IV receptor; (ii) during avian development, expression of the alpha 1 integrin subunit is spatially and temporally regulated; (iii) during myogenesis and neurogenesis, expression of alpha 1 integrin is transient and correlates with cell migration and differentiation.

This article has been cited by other articles:

				G. R. Bolduc and L. C. Madoff The group B streptococcal alpha C protein binds {alpha}1 1-integrin through a novel KTD motif that promotes internalization of GBS within human epithelial cells Microbiology, December 1, 2007; 153(12): 4039 - 4049. [Abstract] [Full Text] [PDF]

				N. Desban, J.-C. Lissitzky, P. Rousselle, and J.-L. Duband {alpha}1{beta}1-integrin engagement to distinct laminin-1 domains orchestrates spreading, migration and survival of neural crest cells through independent signaling pathways J. Cell Sci., August 1, 2006; 119(15): 3206 - 3218. [Abstract] [Full Text] [PDF]

				W. M. Abraham, A. Ahmed, I. Serebriakov, A. N. Carmillo, J. Ferrant, A. R. de Fougerolles, E. A. Garber, P. J. Gotwals, V. E. Koteliansky, F. Taylor, et al. A Monoclonal Antibody to {alpha}1{beta}1 Blocks Antigen-induced Airway Responses in Sheep Am. J. Respir. Crit. Care Med., January 1, 2004; 169(1): 97 - 104. [Abstract] [Full Text] [PDF]

				S. O. Andreasen, A. R. Thomsen, V. E. Koteliansky, T. I. Novobrantseva, A. G. Sprague, A. R. de Fougerolles, and J. P. Christensen Expression and Functional Importance of Collagen-Binding Integrins, {alpha}1{beta}1 and {alpha}2{beta}1, on Virus-Activated T Cells J. Immunol., September 15, 2003; 171(6): 2804 - 2811. [Abstract] [Full Text] [PDF]

				A. M. Mercurio Lessons from the {alpha}2 Integrin Knockout Mouse Am. J. Pathol., July 1, 2002; 161(1): 3 - 6. [Full Text] [PDF]

				C. Stipp and M. Hemler Transmembrane-4-superfamily proteins CD151 and CD81 associate with alpha 3 beta 1 integrin, and selectively contribute to alpha 3 beta 1-dependent neurite outgrowth J. Cell Sci., January 6, 2000; 113(11): 1871 - 1882. [Abstract] [PDF]

				S Testaz, M Delannet, and J Duband Adhesion and migration of avian neural crest cells on fibronectin require the cooperating activities of multiple integrins of the (beta)1 and (beta)3 families J. Cell Sci., January 12, 1999; 112(24): 4715 - 4728. [Abstract] [PDF]

				H. Ra, C Picart, H Feng, H. Sweeney, and D. Discher Muscle cell peeling from micropatterned collagen: direct probing of focal and molecular properties of matrix adhesion J. Cell Sci., January 5, 1999; 112(10): 1425 - 1436. [Abstract] [PDF]

				H Gardner, A Broberg, A Pozzi, M Laato, and J Heino Absence of integrin alpha1beta1 in the mouse causes loss of feedback regulation of collagen synthesis in normal and wounded dermis J. Cell Sci., January 2, 1999; 112(3): 263 - 272. [Abstract] [PDF]

				J. K. Ivins, H. Colognato, J. A. Kreidberg, P. D. Yurchenco, and A. D. Lander Neuronal Receptors Mediating Responses to AntibodyActivated Laminin-1 J. Neurosci., December 1, 1998; 18(23): 9703 - 9715. [Abstract] [Full Text] [PDF]

				D. Taverna, M.-H. Disatnik, H. Rayburn, R. T. Bronson, J. Yang, T. A. Rando, and R. O. Hynes Dystrophic Muscle in Mice Chimeric for Expression of alpha 5 Integrin J. Cell Biol., November 2, 1998; 143(3): 849 - 859. [Abstract] [Full Text] [PDF]

				D. Franco, W. H Lamers, and A. F.M Moorman Patterns of expression in the developing myocardium: towards a morphologically integrated transcriptional model Cardiovasc Res, April 1, 1998; 38(1): 25 - 53. [Full Text] [PDF]

				U. Treubert and T. Brummendorf Functional Cooperation of beta 1-Integrins and Members of the Ig Superfamily in Neurite Outgrowth Induction J. Neurosci., March 1, 1998; 18(5): 1795 - 1805. [Abstract] [Full Text] [PDF]

				E Hirsch, L Lohikangas, D Gullberg, S Johansson, and R Fassler Mouse myoblasts can fuse and form a normal sarcomere in the absence of beta1 integrin expression J. Cell Sci., January 8, 1998; 111(16): 2397 - 2409. [Abstract] [PDF]

				R. Wang, M. H. Stromer, and T. W. Huiatt Integrin Expression in Developing Smooth Muscle Cells J. Histochem. Cytochem., January 1, 1998; 46(1): 119 - 126. [Abstract] [Full Text]

				H. Colognato, M. MacCarrick, J. J. O'Rear, and P. D. Yurchenco The Laminin alpha 2-Chain Short Arm Mediates Cell Adhesion through Both the alpha 1beta 1 and alpha 2beta 1 Integrins J. Biol. Chem., November 14, 1997; 272(46): 29330 - 29336. [Abstract] [Full Text] [PDF]

				H. Obata, K.'i. Hayashi, W. Nishida, T. Momiyama, A. Uchida, T. Ochi, and K. Sobue Smooth Muscle Cell Phenotype-dependent Transcriptional Regulation of the alpha 1 Integrin Gene J. Biol. Chem., October 17, 1997; 272(42): 26643 - 26651. [Abstract] [Full Text] [PDF]

				N Desban and J. Duband Avian neural crest cell migration on laminin: interaction of the alpha1beta1 integrin with distinct laminin-1 domains mediates different adhesive responses J. Cell Sci., January 11, 1997; 110(21): 2729 - 2744. [Abstract] [PDF]

				P. Martin and J. Sanes Integrins mediate adhesion to agrin and modulate agrin signaling Development, January 10, 1997; 124(19): 3909 - 3917. [Abstract] [PDF]

				C. Yao, B. Ziober, A. Sutherland, D. Mendrick, and R. Kramer Laminins promote the locomotion of skeletal myoblasts via the alpha 7 integrin receptor J. Cell Sci., January 12, 1996; 109(13): 3139 - 3150. [Abstract] [PDF]

				H. Colognato-Pyke, J. J. O'Rear, Y. Yamada, S. Carbonetto, Y.-S. Cheng, and P. D. Yurchenco Mapping of Network-forming, Heparin-binding, and [IMAGE]1[IMAGE]1 Integrin-recognition Sites within the [IMAGE]-Chain Short Arm of Laminin-1 J. Biol. Chem., April 21, 1995; 270(16): 9398 - 9406. [Abstract] [Full Text] [PDF]

				A. Bradshaw, K. McNagny, D. Gervin, G. Cann, T Graf, and D. Clegg Integrin alpha 2 beta 1 mediates interactions between developing embryonic retinal cells and collagen Development, January 11, 1995; 121(11): 3593 - 3602. [Abstract] [PDF]

				N. Osses and E. Brandan ECM is required for skeletal muscle differentiation independently of muscle regulatory factor expression Am J Physiol Cell Physiol, February 1, 2002; 282(2): C383 - C394. [Abstract] [Full Text] [PDF]