|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 116, Issue 3 755-766, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
MJ Beanan and S Strome
Department of Biology, Indiana University, Bloomington 47405.
The C. elegans germ line is generated by extensive proliferation of the two germ-line progenitor cells present in newly hatched larvae. We describe genetic and phenotypic characterization of glp-4, a locus whose product is required for normal proliferation of the germ line. glp-4(bn2ts) mutant worms raised at the restrictive temperature contain approximately 12 germ nuclei, in contrast to the 700-1000 present in wild-type adults. The few germ cells present in sterile glp-4 adults appear to be arrested at prophase of the mitotic cell cycle. This cell-cycle disruption prevents the germ cells from entering meiosis and differentiating into gametes. Shifting sterile glp-4 worms to the permissive temperature enables their germ cells to undergo extensive proliferation and form gametes, demonstrating that the bn2-induced cell-cycle arrest is reversible and that proliferation and differentiation of germ cells can be uncoupled from development of the somatic gonad. The glp-4(bn2ts) mutation can be used to generate large populations of worms that are severely depleted in germ cells, facilitating determination of whether any gene of interest is expressed in the germ line or soma or both.
This article has been cited by other articles:
|
|
 |
|
  R. E. Muir and M.-W. Tan Virulence of Leucobacter chromiireducens subsp. solipictus to Caenorhabditis elegans: Characterization of a Novel Host-Pathogen Interaction Appl. Envir. Microbiol., July 1, 2008; 74(13): 4185 - 4198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takeda, C. Watanabe, H. Qadota, M. Hanazawa, and A. Sugimoto Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method. Genes Cells, July 1, 2008; 13(7): 653 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Audhya and A. Desai Proteomics in Caenorhabditis elegans Brief Funct Genomic Proteomic, May 1, 2008; 7(3): 205 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. McColl, D. W. Killilea, A. E. Hubbard, M. C. Vantipalli, S. Melov, and G. J. Lithgow Pharmacogenetic Analysis of Lithium-induced Delayed Aging in Caenorhabditis elegans J. Biol. Chem., January 4, 2008; 283(1): 350 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Updike and S. E. Mango Genetic Suppressors of Caenorhabditis elegans pha-4/FoxA Identify the Predicted AAA Helicase ruvb-1/RuvB Genetics, October 1, 2007; 177(2): 819 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu and E. M. Maine The Bro1-Domain Protein, EGO-2, Promotes Notch Signaling in Caenorhabditis elegans Genetics, August 1, 2007; 176(4): 2265 - 2277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Matsubara, I. Kawasaki, S. Urushiyama, T. Yasuda, M. Shirakata, Y. Iino, H. Shibuya, and Y. Yamanashi The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans Genes Cells, March 1, 2007; 12(3): 407 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Paulander, A. Pennhag, D. I. Andersson, and S. Maisnier-Patin Caenorhabditis elegans as a Model To Determine Fitness of Antibiotic-Resistant Salmonella enterica Serovar Typhimurium Antimicrob. Agents Chemother., February 1, 2007; 51(2): 766 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Kritikou, S. Milstein, P.-O. Vidalain, G. Lettre, E. Bogan, K. Doukoumetzidis, P. Gray, T. G. Chappell, M. Vidal, and M. O. Hengartner C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway required for germ cell survival. Genes & Dev., August 15, 2006; 20(16): 2279 - 2292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. I. Moy, A. R. Ball, Z. Anklesaria, G. Casadei, K. Lewis, and F. M. Ausubel From the Cover: Identification of novel antimicrobials using a live-animal infection model PNAS, July 5, 2006; 103(27): 10414 - 10419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Parusel, E. A. Kritikou, M. O. Hengartner, W. Krek, and M. Gotta URI-1 is required for DNA stability in C. elegans Development, February 15, 2006; 133(4): 621 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Suzuki and M. Han Genetic redundancy masks diverse functions of the tumor suppressor gene PTEN during C. elegans development. Genes & Dev., February 15, 2006; 20(4): 423 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Cheung, M. Schertzer, A. Rose, and P. M. Lansdorp High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans Nucleic Acids Res., January 10, 2006; 34(1): 96 - 103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Boag, A. Nakamura, and T. K. Blackwell A conserved RNA-protein complex component involved in physiological germline apoptosis regulation in C. elegans Development, November 15, 2005; 132(22): 4975 - 4986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Maciejowski, J. H. Ahn, P. G. Cipriani, D. J. Killian, A. L. Chaudhary, J. I. Lee, R. Voutev, R. C. Johnsen, D. L. Baillie, K. C. Gunsalus, et al. Autosomal Genes of Autosomal/X-Linked Duplicated Gene Pairs and Germ-Line Proliferation in Caenorhabditis elegans Genetics, April 1, 2005; 169(4): 1997 - 2011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Benard, H. Kebir, S. Takagi, and S. Hekimi mau-2 acts cell-autonomously to guide axonal migrations in Caenorhabditis elegans Development, December 1, 2004; 131(23): 5947 - 5958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. McElwee, E. Schuster, E. Blanc, J. H. Thomas, and D. Gems Shared Transcriptional Signature in Caenorhabditis elegans Dauer Larvae and Long-lived daf-2 Mutants Implicates Detoxification System in Longevity Assurance J. Biol. Chem., October 22, 2004; 279(43): 44533 - 44543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Mootz, D. M. Ho, and C. P. Hunter The STAR/Maxi-KH domain protein GLD-1 mediates a developmental switch in the translational control of C. elegans PAL-1 Development, July 15, 2004; 131(14): 3263 - 3272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kawasaki, A. Amiri, Y. Fan, N. Meyer, S. Dunkelbarger, T. Motohashi, T. Karashima, O. Bossinger, and S. Strome The PGL Family Proteins Associate With Germ Granules and Function Redundantly in Caenorhabditis elegans Germline Development Genetics, June 1, 2004; 167(2): 645 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fridkin, E. Mills, A. Margalit, E. Neufeld, K. K. Lee, N. Feinstein, M. Cohen, K. L. Wilson, and Y. Gruenbaum Matefin, a Caenorhabditis elegans germ line-specific SUN-domain nuclear membrane protein, is essential for early embryonic and germ cell development PNAS, May 4, 2004; 101(18): 6987 - 6992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Reinke, I. S. Gil, S. Ward, and K. Kazmer Genome-wide germline-enriched and sex-biased expression profiles in Caenorhabditis elegans Development, January 15, 2004; 131(2): 311 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Segbert, R. Barkus, J. Powers, S. Strome, W. M. Saxton, and O. Bossinger KLP-18, a Klp2 Kinesin, Is Required for Assembly of Acentrosomal Meiotic Spindles in Caenorhabditis elegans Mol. Biol. Cell, November 1, 2003; 14(11): 4458 - 4469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Lim, N. C. Lau, E. G. Weinstein, A. Abdelhakim, S. Yekta, M. W. Rhoades, C. B. Burge, and D. P. Bartel The microRNAs of Caenorhabditis elegans Genes & Dev., April 15, 2003; 17(8): 991 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Van Auken, D. Weaver, B. Robertson, M. Sundaram, T. Saldi, L. Edgar, U. Elling, M. Lee, Q. Boese, and W. B. Wood Roles of the Homothorax/Meis/Prep homolog UNC-62 and the Exd/Pbx homologs CEH-20 and CEH-40 in C. elegans embryogenesis Development, March 13, 2003; 129(22): 5255 - 5268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mylonakis, F. M. Ausubel, J. R. Perfect, J. Heitman, and S. B. Calderwood Killing of Caenorhabditiselegans by Cryptococcus neoformans as a model of yeast pathogenesis PNAS, November 26, 2002; 99(24): 15675 - 15680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Colaiacovo, G. M. Stanfield, K. C. Reddy, V. Reinke, S. K. Kim, and A. M. Villeneuve A Targeted RNAi Screen for Genes Involved in Chromosome Morphogenesis and Nuclear Organization in the Caenorhabditis elegans Germline Genetics, September 1, 2002; 162(1): 113 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Kim, R. Feinbaum, G. Alloing, F. E. Emerson, D. A. Garsin, H. Inoue, M. Tanaka-Hino, N. Hisamoto, K. Matsumoto, M.-W. Tan, et al. A Conserved p38 MAP Kinase Pathway in Caenorhabditis elegans Innate Immunity Science, July 26, 2002; 297(5581): 623 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fong, L. Bender, W. Wang, and S. Strome Regulation of the Different Chromatin States of Autosomes and X Chromosomes in the Germ Line of C. elegans Science, June 21, 2002; 296(5576): 2235 - 2238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu and S. Strome Depletion of a Novel SET-Domain Protein Enhances the Sterility of mes-3 and mes-4 Mutants of Caenorhabditis elegans Genetics, November 1, 2001; 159(3): 1019 - 1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. C. Lau, L. P. Lim, E. G. Weinstein, and D. P. Bartel An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans Science, October 26, 2001; 294(5543): 858 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Amiri, B. D. Keiper, I. Kawasaki, Y. Fan, Y. Kohara, R. E. Rhoads, and S. Strome An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegans Development, October 15, 2001; 128(20): 3899 - 3912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Benard, B. McCright, Y. Zhang, S. Felkai, B. Lakowski, and S. Hekimi The C. elegans maternal-effect gene clk-2 is essential for embryonic development, encodes a protein homologous to yeast Tel2p and affects telomere length Development, October 15, 2001; 128(20): 4045 - 4055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Navarro, E. Y. Shim, Y. Kohara, A. Singson, and T. K. Blackwell cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans Development, September 1, 2001; 128(17): 3221 - 3232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hanazawa, M. Mochii, N. Ueno, Y. Kohara, and Y. Iino Use of cDNA subtraction and RNA interference screens in combination reveals genes required for germ-line development in Caenorhabditis elegans PNAS, July 5, 2001; (2001) 141004698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Golden, P. L. Sadler, M. R. Wallenfang, J. M. Schumacher, D. R. Hamill, G. Bates, B. Bowerman, G. Seydoux, and D. C. Shakes Metaphase to Anaphase (mat) Transition-defective Mutants in Caenorhabditis elegans J. Cell Biol., December 27, 2000; 151(7): 1469 - 1482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takanami, A. Mori, H. Takahashi, and A. Higashitani Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans Nucleic Acids Res., November 1, 2000; 28(21): 4232 - 4236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Furuta, S. Tuck, J. Kirchner, B. Koch, R. Auty, R. Kitagawa, A. M. Rose, and D. Greenstein EMB-30: An APC4 Homologue Required for Metaphase-to-Anaphase Transitions during Meiosis and Mitosis in Caenorhabditis elegans Mol. Biol. Cell, April 1, 2000; 11(4): 1401 - 1419. [Abstract] [Full Text]
|
|
|
|
|
|
L. Berkowitz and S Strome MES-1, a protein required for unequal divisions of the germline in early C. elegans embryos, resembles receptor tyrosine kinases and is localized to the boundary between the germline and gut cells Development, January 10, 2000; 127(20): 4419 - 4431. [Abstract] [PDF]
|
|
|
|
|
|
T Karashima, A Sugimoto, and M Yamamoto Caenorhabditis elegans homologue of the human azoospermia factor DAZ is required for oogenesis but not for spermatogenesis Development, January 3, 2000; 127(5): 1069 - 1079. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Puoti and J. Kimble The Caenorhabditis elegans Sex Determination Gene mog-1 Encodes a Member of the DEAH-Box Protein Family Mol. Cell. Biol., March 1, 1999; 19(3): 2189 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Subramaniam and G Seydoux nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans Development, January 11, 1999; 126(21): 4861 - 4871. [Abstract] [PDF]
|
|
|
|
|
|
R Holdeman, S Nehrt, and S Strome MES-2, a maternal protein essential for viability of the germline in Caenorhabditis elegans, is homologous to a Drosophila Polycomb group protein Development, January 7, 1998; 125(13): 2457 - 2467. [Abstract] [PDF]
|
|
|
|
|
|
I Korf, Y Fan, and S Strome The Polycomb group in Caenorhabditis elegans and maternal control of germline development Development, January 7, 1998; 125(13): 2469 - 2478. [Abstract] [PDF]
|
|
|
|
|
|
L. Kadyk and J Kimble Genetic regulation of entry into meiosis in Caenorhabditis elegans Development, January 5, 1998; 125(10): 1803 - 1813. [Abstract] [PDF]
|
|
|
|
|
|
The Phenotype of mes-2, mes-3, mes-4 and mes-6, Maternal-Effect Genes Required for Survival of the Germline in Caenorhabditis elegans, Is Sensitive to Chromosome Dosage Genetics, January 1, 1998; 148(1): 167 - 186.
|
|
|
|
|
|
H Browning and S Strome A sperm-supplied factor required for embryogenesis in C. elegans Development, January 1, 1996; 122(1): 391 - 404. [Abstract] [PDF]
|
|
|
|
|
|
A R Jones and T Schedl Mutations in gld-1, a female germ cell-specific tumor suppressor gene in Caenorhabditis elegans, affect a conserved domain also found in Src-associated protein Sam68. Genes & Dev., June 15, 1995; 9(12): 1491 - 1504. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Kreutzer, J. Richards, M. De Silva-Udawatta, J. Temenak, J. Knoblich, C. Lehner, and K. Bennett Caenorhabditis elegans cyclin A- and B-type genes: a cyclin A multigene family, an ancestral cyclin B3 and differential germline expression J. Cell Sci., January 6, 1995; 108(6): 2415 - 2424. [Abstract] [PDF]
|
|
|
|
|
|
D Greenstein, S Hird, R H Plasterk, Y Andachi, Y Kohara, B Wang, M Finney, and G Ruvkun Targeted mutations in the Caenorhabditis elegans POU homeo box gene ceh-18 cause defects in oocyte cell cycle arrest, gonad migration, and epidermal differentiation. Genes & Dev., August 15, 1994; 8(16): 1935 - 1948. [Abstract] [PDF]
|
|
|
|
|
|
P. Okkema and A Fire The Caenorhabditis elegans NK-2 class homeoprotein CEH-22 is involved in combinatorial activation of gene expression in pharyngeal muscle Development, January 8, 1994; 120(8): 2175 - 2186. [Abstract] [PDF]
|
|
|
|
|
|
M. Hanazawa, M. Mochii, N. Ueno, Y. Kohara, and Y. Iino Use of cDNA subtraction and RNA interference screens in combination reveals genes required for germ-line development in Caenorhabditis elegans PNAS, July 17, 2001; 98(15): 8686 - 8691. [Abstract] [Full Text] [PDF]
|
|
