|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 116, Issue 4 915-930, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
J Shih and R Keller
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
In a companion paper (Shih, J. and Keller, R. (1992) Development 116, 901-914), we described a sequence of cell behaviors, called mediolateral intercalation behavior (MIB), that produces mediolateral cell intercalation, the process that drives convergence and extension of the axial and paraxial mesoderm of Xenopus. In this paper, we describe the pattern of expression of MIB in the mesoderm during gastrulation, using video image processing and recording of cell behavior in 'shaved', open-faced explants of the marginal zone. At midgastrula stage (10.5), MIB begins at two dorsolateral sites in the prospective anterior mesoderm and progresses medially along two arcs that lengthen toward and meet at the midline to form a single arc of cells expressing MIB, called the vegetal alignment zone (VgAZ). The notochordal-somitic mesodermal boundary forms within the VgAZ at stage 11, and then progresses animally and laterally, along the prospective anterior-posterior axis, eventually bounding a trapezoidal area the shape of the fate-mapped notochord. Meanwhile, from its origin in the VgAZ, MIB spreads in the prospective posterior direction along the lateral boundaries of both the notochordal and somitic mesoderm. From there it spreads medially in both tissues. Subsequently, vacuolation of notochord cells, and segmentation and expression of a somite-specific marker repeat the progression of mediolateral intercalation behavior. Thus cells in the posterior, medial regions of the notochordal and the somitic territories are the last to express mediolateral intercalation behavior and subsequent tissue differentiations. In explants that do not converge, these cells neither express mediolateral intercalation behavior nor differentiate. These facts suggest that progressions of MIB in the anterior-posterior and lateral-medial directions may be organized by signals emanating from the lateral somitic and notochordal boundaries. These signals may have limited range and may be dependent on convergence, driven by mediolateral cell intercalation, to bring cells within their range. In the embryo, the posterior progression of MIB results in arcs of convergence, anchored in the vegetal endoderm at each end, acting on the inside of the blastoporal lip to produce involution of the IMZ.
This article has been cited by other articles:
|
|
 |
|
  P. Skoglund, A. Rolo, X. Chen, B. M. Gumbiner, and R. Keller Convergence and extension at gastrulation require a myosin IIB-dependent cortical actin network Development, July 15, 2008; 135(14): 2435 - 2444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Veeman, Y. Nakatani, C. Hendrickson, V. Ericson, C. Lin, and W. C. Smith chongmague reveals an essential role for laminin-mediated boundary formation in chordate convergence and extension movements Development, January 1, 2008; 135(1): 33 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sato, D. K. Khadka, W. Liu, R. Bharti, L. W. Runnels, I. B. Dawid, and R. Habas Profilin is an effector for Daam1 in non-canonical Wnt signaling and is required for vertebrate gastrulation Development, November 1, 2006; 133(21): 4219 - 4231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Keller Mechanisms of elongation in embryogenesis Development, June 15, 2006; 133(12): 2291 - 2302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Borchers, Y. Fonar, D. Frank, and J. C. Baker XNF-ATc3 affects neural convergent extension Development, May 1, 2006; 133(9): 1745 - 1755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. E. Reintsch, A. Habring-Mueller, R. W. Wang, A. Schohl, and F. Fagotto {beta}-Catenin controls cell sorting at the notochord-somite boundary independently of cadherin-mediated adhesion J. Cell Biol., August 15, 2005; 170(4): 675 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Ewald, S. M. Peyrot, J. M. Tyszka, S. E. Fraser, and J. B. Wallingford Regional requirements for Dishevelled signaling during Xenopus gastrulation: separable effects on blastopore closure, mesendoderm internalization and archenteron formation Development, December 15, 2004; 131(24): 6195 - 6209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Wallingford and R. M. Harland Neural tube closure requires Dishevelled-dependent convergent extension of the midline Development, March 14, 2003; 129(24): 5815 - 5825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Glickman, C. B. Kimmel, M. A. Jones, and R. J. Adams Shaping the zebrafish notochord Development, March 1, 2003; 130(5): 873 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Habas, I. B. Dawid, and X. He Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation Genes & Dev., January 15, 2003; 17(2): 295 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Keller Shaping the Vertebrate Body Plan by Polarized Embryonic Cell Movements Science, December 6, 2002; 298(5600): 1950 - 1954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Munro and G. M. Odell Polarized basolateral cell motility underlies invagination and convergent extension of the ascidian notochord Development, January 1, 2002; 129(1): 13 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Nutt, K. S. Dingwell, C. E. Holt, and E. Amaya Xenopus Sprouty2 inhibits FGF-mediated gastrulation movements but does not affect mesoderm induction and patterning Genes & Dev., May 1, 2001; 15(9): 1152 - 1166. [Abstract] [Full Text]
|
|
|
|
|
|
P. Niewiadomska, D. Godt, and U. Tepass DE-Cadherin Is Required for Intercellular Motility during Drosophila Oogenesis J. Cell Biol., February 8, 1999; 144(3): 533 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lane and W. Smith The origins of primitive blood in Xenopus: implications for axial patterning Development, January 2, 1999; 126(3): 423 - 434. [Abstract] [PDF]
|
|
|
|
|
|
M. Lane and R Keller Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by the vegetal alignment zone Development, January 2, 1997; 124(4): 895 - 906. [Abstract] [PDF]
|
|
|
|
|
|
S. Moore, R. Keller, and M. Koehl The dorsal involuting marginal zone stiffens anisotropically during its convergent extension in the gastrula of Xenopus laevis Development, January 10, 1995; 121(10): 3131 - 3140. [Abstract] [PDF]
|
|
|
|
|
|
C Domingo and R Keller Induction of notochord cell intercalation behavior and differentiation by progressive signals in the gastrula of Xenopus laevis Development, January 10, 1995; 121(10): 3311 - 3321. [Abstract] [PDF]
|
|
|
|
|
|
J Shih and S. Fraser Distribution of tissue progenitors within the shield region of the zebrafish gastrula Development, January 9, 1995; 121(9): 2755 - 2765. [Abstract] [PDF]
|
|
|
|
|
|
Frontiers in biology: development Science, October 28, 1994; 266(5185): 561 - 614. [PDF]
|
|
|
|
|
|
F Fagotto and B. Gumbiner Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries Development, January 12, 1994; 120(12): 3667 - 3679. [Abstract] [PDF]
|
|
|
|
|
|
Y Hatada and C. Stern A fate map of the epiblast of the early chick embryo Development, January 10, 1994; 120(10): 2879 - 2889. [Abstract] [PDF]
|
|
|
|
|
|
C. Kimmel, R. Warga, and D. Kane Cell cycles and clonal strings during formation of the zebrafish central nervous system Development, January 2, 1994; 120(2): 265 - 276. [Abstract] [PDF]
|
|
