spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, T.
Right arrow Articles by Dziadek, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, T.
Right arrow Articles by Dziadek, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 117, Issue 1 253-262, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Capacity to form choroid plexus-like cells in vitro is restricted to specific regions of the mouse neural ectoderm

T Thomas and M Dziadek
Centre for Early Human Development, Monash Medical Centre, Clayton, Victoria, Australia.

Neural ectoderm was dissected from 9.5-day and 8.5-day gestation mouse embryos and divided into forebrain, midbrain, hindbrain and spinal cord regions. Forebrain and hindbrain material from 9.5-day neural ectoderm was further divided into presumptive choroid plexus regions and regions that would normally form nervous tissue in vivo. All tissues were plated onto a basement membrane substratum for culture in vitro. It was found that explants of neural ectoderm that would normally form choroid plexus in vivo, readily differentiated to form choroid plexus-like cells in culture. Cells from hindbrain segments and forebrain regions, which would normally form nervous tissue, also had the potential to differentiate into cells resembling the choroid plexus epithelium in culture, provided that the normal cell-cell interactions were disrupted. Cells from the midbrain neuromeres of 9.5-day embryos, which do not form a choroid plexus in vivo, did not form this lineage in vitro. However, cells cultured from the earlier head-fold stage midbrain neural ectoderm could develop into choroid plexus epithelium. There was no evidence that neural ectoderm from the spinal cord had the developmental potential to form choroid plexus epithelial cells at either of these two developmental stages. These studies show that the restrictions in the potential of neural ectoderm stem cells to form different lineages proceeds according to morphological divisions that appear along the anterior-posterior axis during the early stages of brain development. These results suggest that the division of neural ectoderm into segments which contain discrete stem cell populations may be a general feature of the early phase of development of the central nervous system.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
X. Huang, T. Ketova, J. T. Fleming, H. Wang, S. K. Dey, Y. Litingtung, and C. Chiang
Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexus
Development, August 1, 2009; 136(15): 2535 - 2543.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
I. Imayoshi, T. Shimogori, T. Ohtsuka, and R. Kageyama
Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline
Development, August 1, 2008; 135(15): 2531 - 2541.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. K. Voss, J. M. Britto, M. P. Dixon, B. N. Sheikh, C. Collin, S.-S. Tan, and T. Thomas
C3G regulates cortical neuron migration, preplate splitting and radial glial cell attachment
Development, June 15, 2008; 135(12): 2139 - 2149.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
N. L. Hunter and S. M. Dymecki
Molecularly and temporally separable lineages form the hindbrain roof plate and contribute differentially to the choroid plexus
Development, October 1, 2007; 134(19): 3449 - 3460.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. D. Merson, M. P. Dixon, C. Collin, R. L. Rietze, P. F. Bartlett, T. Thomas, and A. K. Voss
The transcriptional coactivator querkopf controls adult neurogenesis.
J. Neurosci., November 1, 2006; 26(44): 11359 - 11370.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. S. Currle, X. Cheng, C.-m. Hsu, and E. S. Monuki
Direct and indirect roles of CNS dorsal midline cells in choroid plexus epithelia formation
Development, August 1, 2005; 132(15): 3549 - 3559.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Tole, G. Goudreau, S. Assimacopoulos, and E. A. Grove
Emx2 Is Required for Growth of the Hippocampus But Not for Hippocampal Field Specification
J. Neurosci., April 1, 2000; 20(7): 2618 - 2625.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. Grove, S Tole, J Limon, L Yip, and C. Ragsdale
The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice
Development, January 6, 1998; 125(12): 2315 - 2325.
[Abstract] [PDF]

Home page
 DevelopmentHome page
Y Furuta, D. Piston, and B. Hogan
Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development
Development, January 6, 1997; 124(11): 2203 - 2212.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993