|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 117, Issue 2 471-482, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
T Diekwisch, S David, P Bringas, V Santos and HC Slavkin
Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles 90033.
During tooth development, enamel organ epithelial cells express a tissue-specific gene product (amelogenin) which presumably functions to control calcium hydroxyapatite crystal growth patterns during enamel biomineralization. The present studies were designed to test the hypothesis that amelogenin as a supramolecular aggregate regulates crystal growth during enamel biomineralization. Antisense oligodeoxynucleotide strategy was used in a simple organ culture system to inhibit amelogenin translation. Under these experimental conditions, antisense treatment prior to and during amelogenin expression resulted in inhibition of amelogenin translation products within immunoprecipitated [35S]methionine metabolically labeled proteins. To determine the efficiency of antisense treatment in this model system, digoxigenin-labeled oligodeoxynucleotides were observed to diffuse throughout the tooth explants including the target ameloblast cells within 24 hours. Ultrastructural analyses of amelogenin supramolecular assembly as electron-dense stippled materials in antisense treated cultures demonstrated dysmorphology of the extracellular enamel matrix with a significant reduction in crystal length and width. We conclude that secreted extracellular proteins form a supramolecular aggregate, which controls both the orientation and dimensions of enamel crystal formation during tooth development.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  O. Golonzhka, D. Metzger, J.-M. Bornert, B. K. Bay, M. K. Gross, C. Kioussi, and M. Leid Ctip2/Bcl11b controls ameloblast formation during mammalian odontogenesis PNAS, March 17, 2009; 106(11): 4278 - 4283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Moradian-Oldak The Emergence of "Nanospheres" as Basic Structural Components Adopted by Amelogenin Journal of Dental Research, June 1, 2007; 86(6): 487 - 490. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hatakeyama, T. Sreenath, Y. Hatakeyama, T. Thyagarajan, L. Shum, C. W. Gibson, J. T. Wright, and A. B. Kulkarni The Receptor Activator of Nuclear Factor-{kappa}B Ligand-mediated Osteoclastogenic Pathway Is Elevated in Amelogenin-null Mice J. Biol. Chem., September 12, 2003; 278(37): 35743 - 35748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. L. Zhou and M. L. Snead Identification of CCAAT/Enhancer-binding Protein alpha as a Transactivator of the Mouse Amelogenin Gene J. Biol. Chem., April 14, 2000; 275(16): 12273 - 12280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G.H. Diekwisch, J. Ware, A. G. Fincham, and M. Zeichner-David Immunohistochemical Similarities and Differences between Amelogenin and Tuftelin Gene Products During Tooth Development J. Histochem. Cytochem., June 1, 1997; 45(6): 859 - 866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Deutsch, E. Chityat, M. Hekmati, A. Palmon, Y. Farkash, and L. Dafni High Expression of Human Amelogenin in E. Coli Advances in Dental Research, November 1, 1996; 10(2): 187 - 194. [Abstract] [PDF]
|
|
|
|
|
|
K. Ibaraki-O'Connor, K. Nakata, and M.F. Young Toward Understanding the Function of Amelogenin Using Transgenic Mice Advances in Dental Research, November 1, 1996; 10(2): 208 - 214. [Abstract] [PDF]
|
|
|
|
|
|
C.-C. Hu, C. Zhang, Q. Qian, O.H. Ryu, J. Moradian-Oldak, A.G. Fincham, and J.P. Simmer Cloning, DNA Sequence, and Alternative Splicing of Opossum Amelogenin mRNAs Journal of Dental Research, October 1, 1996; 75(10): 1728 - 1734. [Abstract] [PDF]
|
|
|
|
 |
|
 M Post, P Souza, J Liu, I Tseu, J Wang, M Kuliszewski, and A. Tanswell Keratinocyte growth factor and its receptor are involved in regulating early lung branching Development, January 10, 1996; 122(10): 3107 - 3115. [Abstract] [PDF]
|
|
|
|
|
|
M. Tabata, K Kim, J. Liu, K Yamashita, T Matsumura, J Kato, M Iwamoto, S Wakisaka, K Matsumoto, T Nakamura, et al. Hepatocyte growth factor is involved in the morphogenesis of tooth germ in murine molars Development, January 4, 1996; 122(4): 1243 - 1251. [Abstract] [PDF]
|
|
|
|
|
|
P Souza, L Sedlackova, M Kuliszewski, J Wang, J Liu, I Tseu, M Liu, A. Tanswell, and M Post Antisense oligodeoxynucleotides targeting PDGF-B mRNA inhibit cell proliferation during embryonic rat lung development Development, January 8, 1994; 120(8): 2163 - 2173. [Abstract] [PDF]
|
|
|
|
|
|
M. Durbeej, S. Soderstrom, T. Ebendal, C. Birchmeier, and P. Ekblom Differential expression of neurotrophin receptors during renal development Development, December 1, 1993; 119(4): 977 - 989. [Abstract] [PDF]
|
|
|
|
|
|
Y. L. Zhou, Y. Lei, and M. L. Snead Functional Antagonism between Msx2 and CCAAT/Enhancer-binding Protein alpha in Regulating the Mouse Amelogenin Gene Expression Is Mediated by Protein-Protein Interaction J. Biol. Chem., September 8, 2000; 275(37): 29066 - 29075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Gibson, Z.-A. Yuan, B. Hall, G. Longenecker, E. Chen, T. Thyagarajan, T. Sreenath, J. T. Wright, S. Decker, R. Piddington, et al. Amelogenin-deficient Mice Display an Amelogenesis Imperfecta Phenotype J. Biol. Chem., August 17, 2001; 276(34): 31871 - 31875. [Abstract] [Full Text] [PDF]
|
|
