spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Anderson, R.
Right arrow Articles by Muneoka, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Anderson, R.
Right arrow Articles by Muneoka, K.

Development, Vol 117, Issue 4 1421-1433, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Maintenance of ZPA signaling in cultured mouse limb bud cells

R Anderson, M Landry and K Muneoka
Department of Cell and Molecular Biology, Tulane University, New Orleans, LA 70118.

The positional signal localized to the posterior (zone of polarizing activity or ZPA) region of the vertebrate limb is transiently expressed during development and a decline in ZPA signaling is accelerated when posterior cells are dissociated and cultured in vitro. The evidence that cultured posterior cells display a precocious decline in ZPA signaling when compared to in vivo studies suggests that a factor present in the limb bud maintains or stabilizes ZPA signaling during limb outgrowth and that this maintenance factor is lost and/or exhausted in in vitro studies. We have developed a new culture technique, 'microdissociation', which preserves extracellular components that we have found to be necessary for ZPA signal maintenance. Our data suggest that the limb bud ectoderm produces a maintenance activity that becomes stored in the extracellular matrix where it acts on limb bud cells to stabilize the activity of the ZPA signal. Using our initial characterization of this maintenance activity, we have identified a growth factor, FGF-2 (bFGF), that can replace all of the ZPA signaling maintenance activity observed in microdissociate cultures. The existence of various members of the FGF family in the developing limb strongly argues a role for FGF in stabilizing ZPA signaling in vivo.


This article has been cited by other articles:


Home page
 DevelopmentHome page
A. Moon, A. Boulet, and M. Capecchi
Normal limb development in conditional mutants of Fgf4
Development, January 3, 2000; 127(5): 989 - 996.
[Abstract] [PDF]

Home page
 JCBHome page
H. Makarenkova, D.L. Becker, C. Tickle, and A.E. Warner
Fibroblast Growth Factor 4 Directs Gap Junction Expression in the Mesenchyme of the Vertebrate Limb Bud
J. Cell Biol., September 8, 1997; 138(5): 1125 - 1137.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Stott and C. Chuong
Dual action of sonic hedgehog on chondrocyte hypertrophy: retrovirus mediated ectopic sonic hedgehog expression in limb bud micromass culture induces novel cartilage nodules that are positive for alkaline phosphatase and type X collagen
J. Cell Sci., January 11, 1997; 110(21): 2691 - 2701.
[Abstract] [PDF]

Home page
 DevelopmentHome page
C. Dealy and R. Kosher
IGF-I, insulin and FGFs induce outgrowth of the limb buds of amelic mutant chick embryos
Development, January 4, 1996; 122(4): 1323 - 1330.
[Abstract] [PDF]

Home page
 DevelopmentHome page
A Vogel and C Tickle
FGF-4 maintains polarizing activity of posterior limb bud cells in vivo and in vitro
Development, January 9, 1993; 119(1): 199 - 206.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993