|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 118, Issue 1 127-138, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
MB Melnick, LA Perkins, M Lee, L Ambrosio and N Perrimon
Department of Genetics, Howard Hughes Medical Institute, Boston, Massachusetts 02115.
Formation of the tail region of the Drosophila larva requires the activities of the terminal class genes. Genetic and molecular analyses of these genes suggests that localized activation of the receptor tyrosine kinase torso at the posterior egg pole triggers a signal transduction pathway. This pathway, mediated through the serine/threonine protein kinase D-raf and the protein tyrosine phosphatase corkscrew, controls the domains of expression of the transcription factors tailless and huckebein. In this paper, we report the molecular and developmental characterization of mutations in the D-raf gene. We show that mutations that alter conserved residues known to be necessary for kinase activity are associated with a null phenotype, demonstrating that D-raf kinase activity is required for its role in torso signaling. Another mutation, D-rafPB26, which prematurely truncates the kinase domain shows a weaker maternal effect phenotype that is strikingly similar to the corkscrew maternal effect phenotype, suggesting that a lower amount of kinase activity decreases the terminal signaling pathway. Finally, molecular and developmental characterization of two mutations that affect the late D-raf zygotic function(s) implies a novel role for D-raf in cell fate establishment in the eye. One of these mutations, D-rafC110, is associated with a single amino acid change within the putative D-raf regulatory region, while the other, D-rafHM-7, most likely reduces the wild-type amount of D-raf protein.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. E. Rocheleau, K. Cullison, K. Huang, Y. Bernstein, A. C. Spilker, and M. V. Sundaram The Caenorhabditis elegans ekl (Enhancer of ksr-1 Lethality) Genes Include Putative Components of a Germline Small RNA Pathway Genetics, March 1, 2008; 178(3): 1431 - 1443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Smit, E. Limpens, R. Geurts, E. Fedorova, E. Dolgikh, C. Gough, and T. Bisseling Medicago LYK3, an Entry Receptor in Rhizobial Nodulation Factor Signaling Plant Physiology, September 1, 2007; 145(1): 183 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li and W. X. Li Drosophila Gain-of-Function Mutant RTK Torso Triggers Ectopic Dpp and STAT Signaling Genetics, May 1, 2003; 164(1): 247 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. X. Li, H. Agaisse, B. Mathey-Prevot, and N. Perrimon Differential requirement for STAT by gain-of-function and wild-type receptor tyrosine kinase Torso in Drosophila Development, March 11, 2003; 129(18): 4241 - 4248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. del Alamo, J. Terriente, and F. J. Diaz-Benjumea Spitz/EGFr signalling via the Ras/MAPK pathway mediates the induction of bract cells in Drosophila legs Development, March 6, 2003; 129(8): 1975 - 1982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Hsu, C. L. Zobel, E. J. Lambie, T. Schedl, and K. Kornfeld Caenorhabditis elegans lin-45 raf Is Essential for Larval Viability, Fertility and the Induction of Vulval Cell Fates Genetics, February 1, 2002; 160(2): 481 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Radke, K. Johnson, R. Guo, A. Davidson, and L. Ambrosio Drosophila-Raf Acts to Elaborate Dorsoventral Pattern in the Ectoderm of Developing Embryos Genetics, November 1, 2001; 159(3): 1031 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-J. Kwon, E.-J. Oh, Y.-S. Kim, F. Hirose, K. Ohno, Y. Nishida, A. Matsukage, M. Yamaguchi, and M.-A. Yoo E2F-dependent transcription of the raf proto-oncogene during Drosophila development Nucleic Acids Res., April 15, 2001; 29(8): 1808 - 1814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Halfar, C Rommel, H Stocker, and E Hafen Ras controls growth, survival and differentiation in the Drosophila eye by different thresholds of MAP kinase activity Development, January 5, 2001; 128(9): 1687 - 1696. [Abstract] [PDF]
|
|
|
|
|
|
L Yang and N. Baker Role of the EGFR/Ras/Raf pathway in specification of photoreceptor cells in the Drosophila retina Development, January 4, 2001; 128(7): 1183 - 1191. [Abstract] [PDF]
|
|
|
|
|
|
F Wittwer, A van der Straten, K Keleman, B. Dickson, and E Hafen Lilliputian: an AF4/FMR2-related protein that controls cell identity and cell growth Development, January 3, 2001; 128(5): 791 - 800. [Abstract] [PDF]
|
|
|
|
|
|
A. Tang, T. Neufeld, G. Rubin, and H. Muller Transcriptional regulation of cytoskeletal functions and segmentation by a novel maternal pair-rule gene, lilliputian Development, January 3, 2001; 128(5): 801 - 813. [Abstract] [PDF]
|
|
|
|
|
|
M. Therrien, D. K. Morrison, A. M. Wong, and G. M. Rubin A Genetic Screen for Modifiers of a Kinase Suppressor of Ras-Dependent Rough Eye Phenotype in Drosophila Genetics, November 1, 2000; 156(3): 1231 - 1242. [Abstract] [Full Text]
|
|
|
|
|
|
W. Li, E. Noll, and N. Perrimon Identification of Autosomal Regions Involved in Drosophila Raf Function Genetics, October 1, 2000; 156(2): 763 - 774. [Abstract] [Full Text]
|
|
|
|
|
|
A. Taguchi, K. Sawamoto, and H. Okano Mutations Modulating the Argos-Regulated Signaling Pathway in Drosophila Eye Development Genetics, April 1, 2000; 154(4): 1639 - 1648. [Abstract] [Full Text]
|
|
|
|
|
|
Q. Zhang, Q. Zheng, and X. Lu A Genetic Screen for Modifiers of Drosophila Src42A Identifies Mutations in Egfr, rolled and a Novel Signaling Gene Genetics, February 1, 1999; 151(2): 697 - 711. [Abstract] [Full Text]
|
|
|
|
|
|
S Greenwood and G Struhl Progression of the morphogenetic furrow in the Drosophila eye: the roles of Hedgehog, Decapentaplegic and the Raf pathway Development, January 12, 1999; 126(24): 5795 - 5808. [Abstract] [PDF]
|
|
|
|
|
|
W Li, M Melnick, and N Perrimon Dual function of Ras in Raf activation Development, January 12, 1998; 125(24): 4999 - 5008. [Abstract] [PDF]
|
|
|
|
|
|
F Roch, A Baonza, E Martin-Blanco, and A Garcia-Bellido Genetic interactions and cell behaviour in blistered mutants during proliferation and differentiation of the Drosophila wing Development, January 5, 1998; 125(10): 1823 - 1832. [Abstract] [PDF]
|
|
|
|
 |
|
 H C Chang and G M Rubin 14-3-3 epsilon positively regulates Ras-mediated signaling in Drosophila. Genes & Dev., May 1, 1997; 11(9): 1132 - 1139. [Abstract] [PDF]
|
|
|
|
|
|
W Li, E. Skoulakis, R. Davis, and N Perrimon The Drosophila 14-3-3 protein Leonardo enhances Torso signaling through D-Raf in a Ras 1-dependent manner Development, January 10, 1997; 124(20): 4163 - 4171. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Ghosh, J. C. Strum, V. A. Sciorra, L. Daniel, and R. M. Bell Raf-1 Kinase Possesses Distinct Binding Domains for Phosphatidylserine and Phosphatidic Acid J. Biol. Chem., April 5, 1996; 271(14): 8472 - 8480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D A Wassarman, N M Solomon, H C Chang, F D Karim, M Therrien, and G M Rubin Protein phosphatase 2A positively and negatively regulates Ras1-mediated photoreceptor development in Drosophila. Genes & Dev., February 1, 1996; 10(3): 272 - 278. [Abstract] [PDF]
|
|
|
|
|
|
R Schweitzer, M Shaharabany, R Seger, and B Z Shilo Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination. Genes & Dev., June 15, 1995; 9(12): 1518 - 1529. [Abstract] [PDF]
|
|
|
|
|
|
C. W. M. Reuter, A. D. Catling, T. Jelinek, and M. J. Weber Biochemical Analysis of MEK Activation in NIH3T3 Fibroblasts J. Biol. Chem., March 31, 1995; 270(13): 7644 - 7655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Andjelkovic, P. F. Jones, U. Grossniklaus, P. Cron, A. F. Schier, M. Dick, G. Bilbe, and B. A. Hemmings Developmental Regulation of Expression and Activity of Multiple Forms of the Drosophila RAC Protein Kinase J. Biol. Chem., February 24, 1995; 270(8): 4066 - 4075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J C Hsu and N Perrimon A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. Genes & Dev., September 15, 1994; 8(18): 2176 - 2187. [Abstract] [PDF]
|
|
|
|
|
|
J Rusch and M Levine Regulation of the dorsal morphogen by the Toll and torso signaling pathways: a receptor tyrosine kinase selectively masks transcriptional repression. Genes & Dev., June 1, 1994; 8(11): 1247 - 1257. [Abstract] [PDF]
|
|
|
|
|
|
A H Brand and N Perrimon Raf acts downstream of the EGF receptor to determine dorsoventral polarity during Drosophila oogenesis. Genes & Dev., March 1, 1994; 8(5): 629 - 639. [Abstract] [PDF]
|
|
|
|
|
|
M Reichman-Fried, B Dickson, E Hafen, and B Z Shilo Elucidation of the role of breathless, a Drosophila FGF receptor homolog, in tracheal cell migration. Genes & Dev., February 15, 1994; 8(4): 428 - 439. [Abstract] [PDF]
|
|
|
|
|
|
F. Diaz-Benjumea and E Hafen The sevenless signalling cassette mediates Drosophila EGF receptor function during epidermal development Development, January 3, 1994; 120(3): 569 - 578. [Abstract] [PDF]
|
|
