|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 118, Issue 1 229-240, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
DL Chapman and DJ Wolgemuth
Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032.
A cDNA encoding the murine cyclin B2 (cycB2) was isolated from an adult mouse testis cDNA library as part of studies designed to identify cyclins involved in murine germ cell development. This cycB2 cDNA was then used to examine the pattern of cycB2 expression during male and female germ cell development and in early embryogenesis, and to compare this expression with the previously characterized expression of cycB1. A single 1.7 kb cycB2 transcript was detected by northern blot hybridization analysis of total RNA isolated from midgestation embryos and various adult tissues. Northern blot and in situ hybridization analyses revealed that cycB2 expression in the testis was most abundant in the germ cells, specifically in pachytene spermatocytes. This is in contrast to the highest levels of expression of cycB1 being present in early spermatids. In situ analysis of the ovary revealed cycB2 transcripts in both germ cells and somatic cells, specifically in the oocytes and granulosa cells of growing and mature follicles. The pattern of cycB1 and cycB2 expression in ovulated and fertilized eggs was also examined. While the steady state level of cycB1 and cycB2 signal remained constant in oocytes and ovulated eggs, signal of both appeared to decrease following fertilization. In addition, both cycB1 and cycB2 transcripts were detected in the cells of the inner cell mass and the trophectoderm of the blastocyst. These results demonstrate that there are lineage- and developmental-specific differences in the pattern of the B cyclins in mammalian germ cells, in contrast to the co-expression of B cyclins in the early conceptus.
This article has been cited by other articles:
|
|
 |
|
  S.-H. Park, G.-R. Yu, W.-H. Kim, W.-S. Moon, J.-H. Kim, and D.-G. Kim NF-Y-Dependent Cyclin B2 Expression in Colorectal Adenocarcinoma Clin. Cancer Res., February 1, 2007; 13(3): 858 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. M. Lin, Y. N. Teng, C. L. Chung, W. C. Tsai, Y. H. Lin, J. S. N. Lin, and P. L. Kuo Decreased mRNA transcripts of M-phase promoting factor and its regulators in the testes of infertile men Hum. Reprod., January 1, 2006; 21(1): 138 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Archer, J. Johnson, H.-J. Kim, Q. Ma, H. Mou, V. Daesety, S. Meng, and R. A. Hodin The histone deacetylase inhibitor butyrate downregulates cyclin B1 gene expression via a p21/WAF-1-dependent mechanism in human colon cancer cells Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G696 - G703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Persson, Q. Zhang, X. Y. Wang, S. E Ravnik, S. Muhlrad, and D. J Wolgemuth Distinct roles for the mammalian A-type cyclins during oogenesis Reproduction, October 1, 2005; 130(4): 411 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Wolgemuth, E. Laurion, and K. M. Lele Regulation of the Mitotic and Meiotic Cell Cycles in the Male Germ Line Recent Prog. Horm. Res., January 1, 2002; 57(1): 75 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Draviam, S. Orrechia, M. Lowe, R. Pardi, and J. Pines The Localization of Human Cyclins B1 and B2 Determines CDK1 Substrate Specificity and Neither Enzyme Requires MEK to Disassemble the Golgi Apparatus J. Cell Biol., March 5, 2001; 152(5): 945 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Beumer, H. L. Roepers-Gajadien, I. S. Gademan, H. B. Kal, and D. G. de Rooij Involvement of the D-Type Cyclins in Germ Cell Proliferation and Differentiation in the Mouse Biol Reprod, December 1, 2000; 63(6): 1893 - 1898. [Abstract] [Full Text]
|
|
|
|
|
|
C. Mori, J. W. Allen, D. J. Dix, N. Nakamura, M. Fujioka, K. Toshimori, and E. M. Eddy Completion of Meiosis Is Not Always Required for Acrosome Formation in HSP70-2 Null Mice Biol Reprod, September 1, 1999; 61(3): 813 - 822. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. Besset, K. Rhee, and D. J. Wolgemuth The Cellular Distribution and Kinase Activity of the Cdk Family Member Pctaire1 in the Adult Mouse Brain and Testis Suggest Functions in Differentiation Cell Growth Differ., March 1, 1999; 10(3): 173 - 181. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Brandeis, I. Rosewell, M. Carrington, T. Crompton, M. A. Jacobs, J. Kirk, J. Gannon, and T. Hunt Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero PNAS, April 14, 1998; 95(8): 4344 - 4349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. MacAuley, J. C. Cross, and Z. Werb Reprogramming the Cell Cycle for Endoreduplication in Rodent Trophoblast Cells Mol. Biol. Cell, April 1, 1998; 9(4): 795 - 807. [Abstract] [Full Text]
|
|
|
|
 |
|
 K Rhee, M Brunori, V Besset, R Trousdale, and D. Wolgemuth Expression and potential role of Fsrg1, a murine bromodomain-containing homologue of the Drosophila gene female sterile homeotic J. Cell Sci., January 12, 1998; 111(23): 3541 - 3550. [Abstract] [PDF]
|
|
|
|
 |
|
 D Zhu, D. Dix, and E. Eddy HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes Development, January 8, 1997; 124(15): 3007 - 3014. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Zhang, Z. Wang, and K. Ravid The Cell Cycle in Polyploid Megakaryocytes Is Associated with Reduced Activity of Cyclin B1-dependent Cdc2 Kinase J. Biol. Chem., February 23, 1996; 271(8): 4266 - 4272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Sweeney, M Murphy, M Kubelka, S. Ravnik, C. Hawkins, D. Wolgemuth, and M Carrington A distinct cyclin A is expressed in germ cells in the mouse Development, January 1, 1996; 122(1): 53 - 64. [Abstract] [PDF]
|
|
|
|
|
|
D. Rappolee, C Basilico, Y Patel, and Z Werb Expression and function of FGF-4 in peri-implantation development in mouse embryos Development, January 8, 1994; 120(8): 2259 - 2269. [Abstract] [PDF]
|
|
