|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 118, Issue 1 9-20, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
TV Orenic, LI Held, SW Paddock and SB Carroll
Howard Hughes Medical Institute, Laboratory of Molecular Biology, University of Wisconsin-Madison 53706.
The spatial organization of Drosophila melanogaster epidermal structures in embryos and adults constitutes a classic model system for understanding how the two dimensional arrangement of particular cell types is generated. For example, the legs of the Drosophila melanogaster adult are covered with bristles, which in most segments are arranged in longitudinal rows. Here we elucidate the key roles of two regulatory genes, hairy and achaete, in setting up this periodic bristle pattern. We show that achaete is expressed during pupal leg development in a dynamic pattern which changes, by approximately 6 hours after puparium formation, into narrow longitudinal stripes of 3-4 cells in width, each of which represents a field of cells (proneural field) from which bristle precursor cells are selected. This pattern of gene expression foreshadows the adult bristle pattern and is established in part through the function of the hairy gene, which also functions in patterning other adult sense organs. In pupal legs, hairy is expressed in four longitudinal stripes, located between every other pair of achaete stripes. We show that in the absence of hairy function achaete expression expands into the interstripe regions that normally express hairy, fusing the two achaete stripes and resulting in extra-wide stripes of achaete expression. This misexpression of achaete, in turn, alters the fields of potential bristle precursor cells which leads to the misalignment of bristle rows in the adult. This function of hairy in patterning achaete expression is distinct from that in the wing in which hairy suppresses late expression of achaete but has no effect on the initial patterning of achaete expression. Thus, the leg bristle pattern is apparently regulated at two levels: a global regulation of the hairy and achaete expression patterns which partitions the leg epidermis into striped zones (this study) and a local regulation (inferred from other studies on the selection of neural precursor cells) that involves refinement steps which may control the alignment and spacing of bristle cells within these zones.
This article has been cited by other articles:
|
|
 |
|
  S. Sinha, X. Ling, C. W. Whitfield, C. Zhai, and G. E. Robinson Genome scan for cis-regulatory DNA motifs associated with social behavior in honey bees PNAS, October 31, 2006; 103(44): 16352 - 16357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-K. Bae, T. Shimizu, and M. Hibi Patterning of proneuronal and inter-proneuronal domains by hairy- and enhancer of split-related genes in zebrafish neuroectoderm Development, March 15, 2005; 132(6): 1375 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kwon, R. Hays, J. Fetting, and T. V. Orenic Opposing inputs by Hedgehog and Brinker define a stripe of hairy expression in the Drosophila leg imaginal disc Development, June 1, 2004; 131(11): 2681 - 2692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W Yi, J. Ross, and D Zarkower Mab-3 is a direct tra-1 target gene regulating diverse aspects of C. elegans male sexual development and behavior Development, January 10, 2000; 127(20): 4469 - 4480. [Abstract] [PDF]
|
|
|
|
|
|
E Cau, G Gradwohl, S Casarosa, R Kageyama, and F Guillemot Hes genes regulate sequential stages of neurogenesis in the olfactory epithelium Development, January 6, 2000; 127(11): 2323 - 2332. [Abstract] [PDF]
|
|
|
|
|
|
R Hays, K. Buchanan, C Neff, and T. Orenic Patterning of Drosophila leg sensory organs through combinatorial signaling by hedgehog, decapentaplegic and wingless Development, January 7, 1999; 126(13): 2891 - 2899. [Abstract] [PDF]
|
|
|
|
|
|
P Simpson, R Woehl, and K Usui The development and evolution of bristle patterns in Diptera Development, January 4, 1999; 126(7): 1349 - 1364. [Abstract] [PDF]
|
|
|
|
|
|
P. Alifragis, G. Poortinga, S. M. Parkhurst, and C. Delidakis A network of interacting transcriptional regulators involved in Drosophila neural fate specification revealed by the yeast two-hybrid system PNAS, November 25, 1997; 94(24): 13099 - 13104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lai and J. Posakony The Bearded box, a novel 3' UTR sequence motif, mediates negative post-transcriptional regulation of Bearded and Enhancer of split Complex gene expression Development, January 12, 1997; 124(23): 4847 - 4856. [Abstract] [PDF]
|
|
|
|
|
|
L. Wrischnik and C. Kenyon The role of lin-22, a hairy/enhancer of split homolog, in patterning the peripheral nervous system of C. elegans Development, January 8, 1997; 124(15): 2875 - 2888. [Abstract] [PDF]
|
|
|
|
 |
|
 J L Gomez-Skarmeta, I Rodriguez, C Martinez, J Culi, D Ferres-Marco, D Beamonte, and J Modolell Cis-regulation of achaete and scute: shared enhancer-like elements drive their coexpression in proneural clusters of the imaginal discs. Genes & Dev., August 1, 1995; 9(15): 1869 - 1882. [Abstract] [PDF]
|
|
|
|
|
|
E. Rulifson and S. Blair Notch regulates wingless expression and is not required for reception of the paracrine wingless signal during wing margin neurogenesis in Drosophila Development, January 9, 1995; 121(9): 2813 - 2824. [Abstract] [PDF]
|
|
|
|
|
|
M Van Doren, A M Bailey, J Esnayra, K Ede, and J W Posakony Negative regulation of proneural gene activity: hairy is a direct transcriptional repressor of achaete. Genes & Dev., November 15, 1994; 8(22): 2729 - 2742. [Abstract] [PDF]
|
|
