|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 118, Issue 2 499-507, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
H Steinbeisser, EM De Robertis, M Ku, DS Kessler and DA Melton
Molecular Biology Institute, University of California, Los Angeles 90024-1737.
In this study, we compare the effects of three mRNAs-goosecoid, activin and Xwnt-8- that are able to induce partial or complete secondary axes when injected into Xenopus embryos. Xwnt-8 injection produces complete secondary axes including head structures whereas activin and goosecoid injection produce partial secondary axes at high frequency that lack head structures anterior to the auditory vesicle and often lack notochord. Xwnt-8 can activate goosecoid only in the deep marginal zone, i.e., in the region in which this organizer-specific homeobox gene is normally expressed on the dorsal side. Activin B mRNA, however, can turn on goosecoid in all regions of the embryo. We also tested the capacity of these gene products to restore axis formation in embryos in which the cortical rotation was blocked by UV irradiation. Whereas Xwnt-8 gives complete rescue of anterior structures, both goosecoid and activin give partial rescue. Rescued axes including hindbrain structures up to level of the auditory vesicle can be obtained at high frequency even in the absence of notochord structures. The possible functions of Wnt-like and activin-like signals and of the goosecoid homeobox gene, and their order of action in the formation of Spemann's organizer are discussed.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Cao, D. Siegel, F. Oswald, and W. Knochel Oct25 Represses Transcription of Nodal/Activin Target Genes by Interaction with Signal Transducers during Xenopus Gastrulation J. Biol. Chem., December 5, 2008; 283(49): 34168 - 34177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Houston, M. Kofron, E. Resnik, R. Langland, O. Destree, C. Wylie, and J. Heasman Repression of organizer genes in dorsal and ventral Xenopus cells mediated by maternal XTcf3 Development, September 1, 2002; 129(17): 4015 - 4025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Xanthos, M. Kofron, Q. Tao, K. Schaible, C. Wylie, and J. Heasman The roles of three signaling pathways in the formation and function of the Spemann Organizer Development, September 1, 2002; 129(17): 4027 - 4043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gradl, A. Konig, and D. Wedlich Functional Diversity of Xenopus Lymphoid Enhancer Factor/T-cell Factor Transcription Factors Relies on Combinations of Activating and Repressing Elements J. Biol. Chem., April 12, 2002; 277(16): 14159 - 14171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Falchuk, J. M. Contin, T. S. Dziedzic, Z. Feng, T. C. French, G. J. Heffron, and M. Montorzi A role for biliverdin IXalpha in dorsal axis development of Xenopus laevis embryos PNAS, January 8, 2002; 99(1): 251 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Skromne and C. D. Stern Interactions between Wnt and Vg1 signalling pathways initiate primitive streak formation in the chick embryo Development, August 1, 2001; 128(15): 2915 - 2927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Montross, H Ji, and P. McCrea A beta-catenin/engrailed chimera selectively suppresses Wnt signaling J. Cell Sci., May 1, 2000; 113(10): 1759 - 1770. [Abstract] [PDF]
|
|
|
|
|
|
E Agius, M Oelgeschlager, O Wessely, C Kemp, and E. De Robertis Endodermal Nodal-related signals and mesoderm induction in Xenopus Development, January 3, 2000; 127(6): 1173 - 1183. [Abstract] [PDF]
|
|
|
|
|
|
S. Kim, A Yamamoto, T Bouwmeester, E Agius, and E. Robertis The role of paraxial protocadherin in selective adhesion and cell movements of the mesoderm during Xenopus gastrulation Development, January 12, 1998; 125(23): 4681 - 4690. [Abstract] [PDF]
|
|
|
|
|
|
B Ferreiro, M Artinger, K Cho, and C Niehrs Antimorphic goosecoids Development, January 4, 1998; 125(8): 1347 - 1359. [Abstract] [PDF]
|
|
|
|
|
|
J. B. A. Green, T. L. Cook, J. C. Smith, and R. M. Grainger Anteroposterior neural tissue specification by activin-induced mesoderm PNAS, August 5, 1997; 94(16): 8596 - 8601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Conway, A. Margoliath, S. Wong-Madden, R. J. Roberts, and W. Gilbert Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos PNAS, April 1, 1997; 94(7): 3082 - 3087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Fagotto, K Guger, and B. Gumbiner Induction of the primary dorsalizing center in Xenopus by the Wnt/GSK/beta-catenin signaling pathway, but not by Vg1, Activin or Noggin Development, January 1, 1997; 124(2): 453 - 460. [Abstract] [PDF]
|
|
|
|
|
|
J Zhang and M. King Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning Development, January 12, 1996; 122(12): 4119 - 4129. [Abstract] [PDF]
|
|
|
|
|
|
A Goriely, M Stella, C Coffinier, D Kessler, C Mailhos, S Dessain, and C Desplan A functional homologue of goosecoid in Drosophila Development, January 5, 1996; 122(5): 1641 - 1650. [Abstract] [PDF]
|
|
|
|
|
|
J. Rivera-Perez, M Mallo, M Gendron-Maguire, T Gridley, and R. Behringer Goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development Development, January 9, 1995; 121(9): 3005 - 3012. [Abstract] [PDF]
|
|
|
|
|
|
G. Kelly, P Greenstein, D. Erezyilmaz, and R. Moon Zebrafish wnt8 and wnt8b share a common activity but are involved in distinct developmental pathways Development, January 6, 1995; 121(6): 1787 - 1799. [Abstract] [PDF]
|
|
|
|
|
|
T Yamada Caudalization by the amphibian organizer: brachyury, convergent extension and retinoic acid Development, January 11, 1994; 120(11): 3051 - 3062. [Abstract] [PDF]
|
|
|
|
|
|
D. Bauer, S Huang, and S. Moody The cleavage stage origin of Spemann's Organizer: analysis of the movements of blastomere clones before and during gastrulation in Xenopus Development, January 5, 1994; 120(5): 1179 - 1189. [Abstract] [PDF]
|
|
|
|
|
|
P Lemaire and J. Gurdon A role for cytoplasmic determinants in mesoderm patterning: cell-autonomous activation of the goosecoid and Xwnt-8 genes along the dorsoventral axis of early Xenopus embryos Development, January 5, 1994; 120(5): 1191 - 1199. [Abstract] [PDF]
|
|
