|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 118, Issue 3 829-844, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
CA Burdsal, CH Damsky and RA Pedersen
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
We have examined the role of cell-cell and cell-extracellular matrix (ECM) interactions during mesoderm differentiation and migration at the primitive streak of the mouse embryo with the use of function-perturbing antibodies. Explants of epiblast or mesoderm tissue dissected from the primitive streak of 7.5- to 7.8-day mouse embryos were cultured on a fibronectin substratum in serum-free, chemically defined medium. After 16-24 hours in culture, cells in explants of epiblast exhibited the typical close-packed morphology of epithelia, and the tissue remained as a coherent patch of cells that were shown to express transcripts of the cytokeratin Endo B by in situ analysis. In contrast, cells in explants of primitive streak mesoderm exhibited a greatly flattened, fibroblastic morphology, did not express Endo B transcripts, and migrated away from the center of the explant. As epiblast cells in vivo undergo the epithelial-mesenchymal transition at the primitive streak, they cease expressing the prominent calcium-sensitive cell adhesion molecule E-cadherin (uvomorulin, Cell-CAM 120/80). We asked whether the loss of E-cadherin expression was a passive result of differentiation or if it might play a more causative role in mesoderm differentiation and migration. Culture with function-perturbing antibodies against E-cadherin caused cells within epiblast explants to lose cell-cell contacts, to flatten, and to assume a mesenchymal morphology; they were also induced to migrate. Anti-E-cadherin antibodies had no effect on explants of primitive streak mesoderm. In immunofluorescence studies, anti-E-cadherin-treated epiblast cells ceased to express SSEA-1, a carbohydrate moiety that is lost as mesoderm differentiates from the epiblast in vivo, and they also ceased to express E-cadherin itself. In contrast, these cells began to express the intermediate filament protein vimentin, a cytoskeletal protein characteristic of the primitive streak mesoderm at this stage of development. As epiblast cells differentiate into mesoderm, their predominant adhesive interactions change from cell-cell to cell-substratum. Therefore, we also investigated the adhesive interactions between primitive streak tissues and extracellular matrix (ECM) components. Epiblast explants adhered well to fibronectin, more poorly to laminin and type IV collagen, and not at all to vitronectin. In contrast, mesoderm explants attached well to all these proteins. Furthermore, epiblast, but not mesoderm, displayed an anchorage-dependent viability in culture. After anti-E-cadherin treatment, epiblast cells that had assumed the mesenchymal morphology did attach to vitronectin, another characteristic shared with primitive streak mesoderm.(ABSTRACT TRUNCATED AT 400 WORDS)
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Stepniak, G. L. Radice, and V. Vasioukhin Adhesive and Signaling Functions of Cadherins and Catenins in Vertebrate Development Cold Spring Harb Perspect Biol, November 1, 2009; 1(5): a002949 - a002949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Egea, C. Erlacher, E. Montanez, I. Burtscher, S. Yamagishi, M. Hess, F. Hampel, R. Sanchez, M. T. Rodriguez-Manzaneque, M. R. Bosl, et al. Genetic ablation of FLRT3 reveals a novel morphogenetic function for the anterior visceral endoderm in suppressing mesoderm differentiation Genes & Dev., December 1, 2008; 22(23): 3349 - 3362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ogata, J. Morokuma, T. Hayata, G. Kolle, C. Niehrs, N. Ueno, and K. W.Y. Cho TGF-beta signaling-mediated morphogenesis: modulation of cell adhesion via cadherin endocytosis Genes & Dev., July 15, 2007; 21(14): 1817 - 1831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Lee, N. F. Silva-Gagliardi, U. Tepass, C. J. McGlade, and K. V. Anderson The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo Development, June 1, 2007; 134(11): 2007 - 2016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Furusawa, M. Ikeda, F. Inoue, K. Ohkoshi, T. Hamano, and T. Tokunaga Gene Expression Profiling of Mouse Embryonic Stem Cell Subpopulations Biol Reprod, October 1, 2006; 75(4): 555 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Christiansen and A. K. Rajasekaran Reassessing Epithelial to Mesenchymal Transition as a Prerequisite for Carcinoma Invasion and Metastasis. Cancer Res., September 1, 2006; 66(17): 8319 - 8326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Rakeman and K. V. Anderson Axis specification and morphogenesis in the mouse embryo require Nap1, a regulator of WAVE-mediated actin branching Development, August 15, 2006; 133(16): 3075 - 3083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hisatsune, K. Matsumura, M. Ogawa, A. Uemura, N. Kondo, J. K. Yamashita, H. Katsuta, S. Nishikawa, T. Chiba, and S.-I. Nishikawa High level of endothelial cell-specific gene expression by a combination of the 5' flanking region and the 5' half of the first intron of the VE-cadherin gene Blood, June 15, 2005; 105(12): 4657 - 4663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Ng, L. Azzola, K. Sourris, L. Robb, E. G. Stanley, and A. G. Elefanty The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells Development, March 1, 2005; 132(5): 873 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Peinado, F. Marin, E. Cubillo, H.-J. Stark, N. Fusenig, M. A. Nieto, and A. Cano Snail and E47 repressors of E-cadherin induce distinct invasive and angiogenic properties in vivo J. Cell Sci., June 1, 2004; 117(13): 2827 - 2839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fagman, M. Grande, J. Edsbagge, H. Semb, and M. Nilsson Expression of Classical Cadherins in Thyroid Development: Maintenance of an Epithelial Phenotype throughout Organogenesis Endocrinology, August 1, 2003; 144(8): 3618 - 3624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bolos, H. Peinado, M. A. Perez-Moreno, M. F. Fraga, M. Esteller, and A. Cano The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions: a comparison with Snail and E47 repressors J. Cell Sci., February 1, 2003; 116(3): 499 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Amieux, D. G. Howe, H. Knickerbocker, D. C. Lee, T. Su, G. S. Laszlo, R. L. Idzerda, and G. S. McKnight Increased Basal cAMP-dependent Protein Kinase Activity Inhibits the Formation of Mesoderm-derived Structures in the Developing Mouse Embryo J. Biol. Chem., July 19, 2002; 277(30): 27294 - 27304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Heymann, I. About, U. Lendahl, J.-C. Franquin, B. Obrink, and T. A. Mitsiadis E- and N-Cadherin Distribution in Developing and Functional Human Teeth under Normal and Pathological Conditions Am. J. Pathol., June 1, 2002; 160(6): 2123 - 2133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Carver, R. Jiang, Y. Lan, K. F. Oram, and T. Gridley The Mouse Snail Gene Encodes a Key Regulator of the Epithelial-Mesenchymal Transition Mol. Cell. Biol., December 1, 2001; 21(23): 8184 - 8188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Sorkin, M. Y. Wang, J. M. Dobeck, K. L. Albergo, and Z. Skobe The Cadherin-Catenin Complex Is Expressed Alternately with the Adenomatous Polyposis Coli Protein During Rat Incisor Amelogenesis J. Histochem. Cytochem., March 1, 2000; 48(3): 397 - 406. [Abstract] [Full Text]
|
|
|
|
|
|
P. Miettinen, M Huotari, T Koivisto, J Ustinov, J Palgi, S Rasilainen, E Lehtonen, J Keski-Oja, and T Otonkoski Impaired migration and delayed differentiation of pancreatic islet cells in mice lacking EGF-receptors Development, January 6, 2000; 127(12): 2617 - 2627. [Abstract] [PDF]
|
|
|
|
|
|
S Orsulic and R Kemler Expression of Eph receptors and ephrins is differentially regulated by E-cadherin J. Cell Sci., January 5, 2000; 113(10): 1793 - 1802. [Abstract] [PDF]
|
|
|
|
|
|
X. Sun, E. N. Meyers, M. Lewandoski, and G. R. Martin Targeted disruption of Fgf8 causes failure of cell migration in the gastrulating mouse embryo Genes & Dev., July 15, 1999; 13(14): 1834 - 1846. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Niewiadomska, D. Godt, and U. Tepass DE-Cadherin Is Required for Intercellular Motility during Drosophila Oogenesis J. Cell Biol., February 8, 1999; 144(3): 533 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zhu and F. Watt beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes independently of intercellular adhesion Development, January 5, 1999; 126(10): 2285 - 2298. [Abstract] [PDF]
|
|
|
|
|
|
N. Nakayama, I. Fang, and G. Elliott Natural Killer and B-Lymphoid Potential in CD34+ Cells Derived From Embryonic Stem Cells Differentiated in the Presence of Vascular Endothelial Growth Factor Blood, April 1, 1998; 91(7): 2283 - 2295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nishikawa, S Nishikawa, M Hirashima, N Matsuyoshi, and H Kodama Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages Development, January 5, 1998; 125(9): 1747 - 1757. [Abstract] [PDF]
|
|
|
|
|
|
C. Wu, S. Y. Keightley, C. Leung-Hagesteijn, G. Radeva, M. Coppolino, S. Goicoechea, J. A. McDonald, and S. Dedhar Integrin-linked Protein Kinase Regulates Fibronectin Matrix Assembly, E-cadherin Expression, and Tumorigenicity J. Biol. Chem., January 2, 1998; 273(1): 528 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ciruna, L Schwartz, K Harpal, T. Yamaguchi, and J Rossant Chimeric analysis of fibroblast growth factor receptor-1 (Fgfr1) function: a role for FGFR1 in morphogenetic movement through the primitive streak Development, January 7, 1997; 124(14): 2829 - 2841. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Rossant, B. Ciruna, and J. Partanen FGF Signaling in Mouse Gastrulation and Anteroposterior Patterning Cold Spring Harb Symp Quant Biol, January 1, 1997; 62(0): 127 - 133. [Abstract] [PDF]
|
|
|
|
|
|
P Dvorak, J. Flechon, E. Thompson, V Horak, P Adenot, and J. Renard Embryoglycans regulate FGF-2-mediated mesoderm induction in the rabbit embryo J. Cell Sci., January 1, 1997; 110(1): 1 - 10. [Abstract] [PDF]
|
|
|
|
|
|
S. Klein, A. Bikfalvi, T. M. Birkenmeier, F. G. Giancotti, and D. B. Rifkin Integrin Regulation by Endogenous Expression of 18-kDa Fibroblast Growth Factor-2 J. Biol. Chem., September 13, 1996; 271(37): 22583 - 22590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Eisenberg and D. M. Bader Establishment of the Mesodermal Cell Line QCE-6 : A Model System for Cardiac Cell Differentiation Circ. Res., February 1, 1996; 78(2): 205 - 216. [Abstract] [Full Text]
|
|
|
|
|
|
M Kanatsu and S. Nishikawa In vitro analysis of epiblast tissue potency for hematopoietic cell differentiation Development, January 3, 1996; 122(3): 823 - 830. [Abstract] [PDF]
|
|
|
|
|
|
J. Cross, M. Flannery, M. Blanar, E Steingrimsson, N. Jenkins, N. Copeland, W. Rutter, and Z Werb Hxt encodes a basic helix-loop-helix transcription factor that regulates trophoblast cell development Development, January 8, 1995; 121(8): 2513 - 2523. [Abstract] [PDF]
|
|
|
|
|
|
E. Newman-Smith and Z Werb Stem cell defects in parthenogenetic peri-implantation embryos Development, January 7, 1995; 121(7): 2069 - 2077. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Nieto, M. Sargent, D. Wilkinson, and J Cooke Control of cell behavior during vertebrate development by Slug, a zinc finger gene Science, May 6, 1994; 264(5160): 835 - 839. [Abstract] [PDF]
|
|
|
|
|
|
F. Conlon, K. Lyons, N Takaesu, K. Barth, A Kispert, B Herrmann, and E. Robertson A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse Development, January 7, 1994; 120(7): 1919 - 1928. [Abstract] [PDF]
|
|
|
|
|
|
B. Holdener, C Faust, N. Rosenthal, and T Magnuson msd is required for mesoderm induction in mice Development, January 5, 1994; 120(5): 1335 - 1346. [Abstract] [PDF]
|
|
