spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Agata, K.
Right arrow Articles by Eguchi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Agata, K.
Right arrow Articles by Eguchi, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 118, Issue 4 1025-1030, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Genetic characterization of the multipotent dedifferentiated state of pigmented epithelial cells in vitro

K Agata, H Kobayashi, Y Itoh, M Mochii, K Sawada and G Eguchi
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.

Retinal pigmented epithelial cells (PECs) of chicken embryos extensively and almost synchronously transdifferentiate into lens cells in medium containing phenylthiourea and testicular hyaluronidase, passing through the bipotent dedifferentiated state. We have isolated genes that are expressed specifically by either pigment or lens cells and analyzed their expression in the transdifferentiation process. The expression of some proto-oncogenes was also studied. In the dedifferentiation process, expression of the c-myc gene was enhanced and the transcription of PEC-specific genes (MMP115, pP344) was completely repressed. However, transcription of lens-specific genes (alpha-, beta- and delta-crystallins genes) remained silent in dedifferentiated pigment cells. Activation of len- or PEC-specific genes occurred only in conditions permissive for lens or PEC differentiation, respectively. These results indicated that lens transdifferentiation from PECs proceeds through a multipotent (or at least bipotent) intermediate cell state in which the c-myc gene is activated, but neither PEC- nor lens-specific genes are expressed.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Sekiguchi-Tonosaki, M. Obata, A. Haruki, T. Himi, and J. Kosaka
Acetylcholine induces Ca2+ signaling in chicken retinal pigmented epithelial cells during dedifferentiation
Am J Physiol Cell Physiol, May 1, 2009; 296(5): C1195 - C1206.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
R.G. Edwards and H. K. Beard
Hypothesis: sex determination and germline formation are committed at the pronucleate stage in mammalian embryos
Mol. Hum. Reprod., July 1, 1999; 5(7): 595 - 606.
[Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
N. Planque, N. Turque, K. Opdecamp, M. Bailly, P. Martin, and S. Saule
Expression of the Microphthalmia-associated Basic Helix-Loop-Helix Leucine Zipper Transcription Factor Miin Avian Neuroretina Cells Induces aPigmented Phenotype
Cell Growth Differ., July 1, 1999; 10(7): 525 - 536.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Toy, J.-M. Yang, G. S. Leppert, and O. H. Sundin
The Optx2 homeobox gene is expressed in early precursors of the eye and activates retina-specific genes
PNAS, September 1, 1998; 95(18): 10643 - 10648.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. Bertelli and M. Bendayan
Intermediate endocrine-acinar pancreatic cells in duct ligation conditions
Am J Physiol Cell Physiol, November 1, 1997; 273(5): C1641 - C1649.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Planque, L. Leconte, F. M. Coquelle, P. Martin, and S. Saule
Specific Pax-6/Microphthalmia Transcription Factor Interactions Involve Their DNA-binding Domains and Inhibit Transcriptional Properties of Both Proteins
J. Biol. Chem., July 27, 2001; 276(31): 29330 - 29337.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1993