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Development, Vol 118, Issue 4 1353-1361, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

A novel H+ permeability dominating intracellular pH in the early mouse embryo

JM Baltz, JD Biggers and C Lechene
Laboratory of Human Reproduction and Reproductive Biology, Harvard Medical School, Boston, MA 02115.

Most cell types are relatively impermeant to H+ and are able to regulate their intracellular pH by means of plasma membrane proteins, which transport H+ or bicarbonate across the membrane in response to perturbations of intracellular pH. Mouse preimplantation embryos at the 2-cell stage, however, do not appear to possess specific pH-regulatory mechanisms for relieving acidosis. They are, instead, highly permeable to H+, so that the intracellular pH in the acid and neutral range is determined by the electrochemical equilibrium of H+ across the plasma membrane. When intracellular pH is perturbed, the rate of the ensuing H+ flux across the plasma membrane is determined by the H+ electrochemical gradient: its dependence on external K+ concentration indicates probable dependence on membrane potential and the rate depends on the H+ concentration gradient across the membrane. The large permeability at the 2-cell stage is absent or greatly diminished in the trophectoderm of blastocysts, but still present in the inner cell mass. Thus, the permeability to H+ appears to be developmentally regulated.


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© The Company of Biologists Ltd 1993