spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poulin, M. L.
Right arrow Articles by Chiu, I. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poulin, M. L.
Right arrow Articles by Chiu, I. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 119, Issue 2 353-361, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Heterogeneity in the expression of fibroblast growth factor receptors during limb regeneration in newts (Notophthalmus viridescens)

ML Poulin, KM Patrie, MJ Botelho, RA Tassava and IM Chiu
Department of Molecular Genetics, Ohio State University, Davis Medical Research Center, Columbus 43210.

Two closely related fibroblast growth factor receptors, FGFR1 and FGFR2, have been cloned from a newt (Notophthalmus viridescens) limb blastema cDNA library. Sequence analysis revealed that we have isolated both the bek and KGFR variants of FGFR2. These two variants differ only in the second half of the last of their three Ig-like domains. The expression patterns of FGFR1 and FGFR2 during limb regeneration have been determined by in situ hybridization. During the preblastema stages of regeneration, FGFR2 expression is observed in the basal layer of the wound epithelium and in the cells of the periosteum. As regeneration progresses to the blastema stages, FGFR2 expression continues to be observed in the basal layer of the wound epithelium with additional hybridization seen in the blastema mesenchyme closely associated with the bisected bones. From the early bud to the mid-bud blastema stage, FGFR1 expression is observed throughout the blastema mesenchyme but, unlike FGFR2, is distinctly absent from the wound epithelium. In the differentiation stages of regeneration, the mesenchymal expression of FGFR2 becomes restricted to the cells of the condensing cartilage and later to the perichondrium. During these later stages of regeneration, the wound epithelium hybridization to the FGFR2 probe is no longer observed. The expression patterns of these receptors suggest that FGFR1 and FGFR2 have distinct roles in limb regeneration, despite their sharing a number of the FGF ligands. Further investigation regarding the potential sources of the FGF ligands will help establish the role that FGFs and FGFRs play in limb regeneration.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J BiochemHome page
T. Ishino, T. Kunieda, S. Natori, K. Sekimizu, and T. Kubo
Identification of Novel Members of the Xenopus Ca2+-dependent Lectin Family and Analysis of Their Gene Expression During Tail Regeneration and Development
J. Biochem., April 1, 2007; 141(4): 479 - 488.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Lee, S. Grill, A. Sanchez, M. Murphy-Ryan, and K. D. Poss
Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration
Development, December 1, 2005; 132(23): 5173 - 5183.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
T. Launay, J.-M. Cabelguen, J.-F. Marini, and C. Chanoine
Effects of locomotor training on hindlimb regeneration in the urodele amphibian Pleurodeles waltlii
J. Physiol., August 15, 2001; 535(1): 241 - 248.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
P. M. Hopkins
Limb Regeneration in the Fiddler Crab, Uca pugilator: Hormonal and Growth Factor Control
Integr. Comp. Biol., June 1, 2001; 41(3): 389 - 398.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Del Rio-Tsonis, J. C. Jung, I.-M. Chiu, and P. A. Tsonis
Conservation of fibroblast growth factor function in lens regeneration
PNAS, December 9, 1997; 94(25): 13701 - 13706.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. Simon, R Kittappa, P. Khan, C Tsilfidis, R. Liversage, and S Oppenheimer
A novel family of T-box genes in urodele amphibian limb development and regeneration: candidate genes involved in vertebrate forelimb/hindlimb patterning
Development, January 4, 1997; 124(7): 1355 - 1366.
[Abstract] [PDF]

Home page
 DevelopmentHome page
L. Mullen, S. Bryant, M. Torok, B Blumberg, and D. Gardiner
Nerve dependency of regeneration: the role of Distal-less and FGF signaling in amphibian limb regeneration
Development, January 11, 1996; 122(11): 3487 - 3497.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Patrie, A. J. Kudla, B. B. Olwin, and I.-M. Chiu
Conservation of Ligand Specificity between the Mammalian and Amphibian Fibroblast Growth Factor Receptors
J. Biol. Chem., December 1, 1995; 270(48): 29018 - 29024.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1993