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Development, Vol 119, Issue 3 813-821, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
T Tada, M Tada and N Takagi
Research Center for Molecular Genetics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
A cytogenetic and biochemical study of balloon-like cystic embryoid bodies, formed by newly established embryonic stem (ES) cell lines having a cytogenetically or genetically marked X chromosome, revealed that the paternally derived X chromosome was inactivated in the majority of cells in the yolk sac-like mural region consisting of the visceral endoderm and mesoderm. The nonrandomness was less evident in the more solid polar region containing the ectodermal vesicle, mesoderm and visceral endoderm. Since the same was true in embryoid bodies derived from ES cells at the 30th subculture generation, it was concluded that the imprinting responsible for the preferential inactivation of the paternal X chromosome that was limited to non-epiblast cells of the female mouse embryos, was stably maintained in undifferentiated ES cells. Differentiating epiblast cells should be able to erase or avoid responding to the imprint.
This article has been cited by other articles:
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  B. C. Popova, T. Tada, N. Takagi, N. Brockdorff, and T. B. Nesterova Attenuated spread of X-inactivation in an X;autosome translocation PNAS, May 16, 2006; 103(20): 7706 - 7711. [Abstract] [Full Text] [PDF]
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 C. Buzin, J. Mann, and J Singer-Sam Quantitative RT-PCR assays show Xist RNA levels are low in mouse female adult tissue, embryos and embryoid bodies Development, January 12, 1994; 120(12): 3529 - 3536. [Abstract] [PDF]
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P. Tam, S. Zhou, and S. Tan X-chromosome activity of the mouse primordial germ cells revealed by the expression of an X-linked lacZ transgene Development, January 10, 1994; 120(10): 2925 - 2932. [Abstract] [PDF]
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