spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Billoud, B.
Right arrow Articles by Angelier, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Billoud, B.
Right arrow Articles by Angelier, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 119, Issue 3 921-932, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Constitutive expression of a somatic heat-inducible hsp70 gene during amphibian oogenesis

B Billoud, ML Rodriguez-Martin, L Berard, N Moreau and N Angelier
Centre de Biologie Cellulaire, CNRS, Ivry-sur-Seine, France.

We isolated and characterized a sequence coding for heat-shock protein 70 (HSP70) of the amphibian Pleurodeles waltl. Results from S1 nuclease protection assays led us to conclude that an hsp70 gene, strictly inducible in somatic cells during heat shock, is constitutively active during oogenesis. By quantitative northern and western blot analysis, we showed that both hsp70 mRNA and HSP70-related protein levels increased in oocytes from stage II to stage VI under physiological conditions. Furthermore, by in situ hybridization to the nascent transcripts of lampbrush chromosome loops, we provided evidence for a clear-cut relationship between this increase in hsp70 mRNA and transcriptional activity during the lampbrush stage of oogenesis. These results strongly suggest that hsp70 genes are actively transcribed throughout oogenesis. HSP70-related proteins localized in the cytoplasm of young oocytes are progressively transferred to the nucleus in the course of oogenesis and preferentially accumulated in the nuclei of some stage VI oocytes.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
E. J. de la Rosa, E. Vega-Nunez, A. V. Morales, J. Serna, E. Rubio, and F. de Pablo
Modulation of the chaperone heat shock cognate 70 by embryonic (pro)insulin correlates with prevention of apoptosis
PNAS, August 18, 1998; 95(17): 9950 - 9955.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R Marino, M. Pinto, F Cotelli, C. Lamia, and R De Santis
The hsp70 protein is involved in the acquisition of gamete self-sterility in the ascidian Ciona intestinalis
Development, January 3, 1998; 125(5): 899 - 907.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993