spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Durbeej, M.
Right arrow Articles by Ekblom, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Durbeej, M.
Right arrow Articles by Ekblom, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 119, Issue 4 977-989, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Differential expression of neurotrophin receptors during renal development

M Durbeej, S Soderstrom, T Ebendal, C Birchmeier and P Ekblom
Department of Animal Physiology, Uppsala University, Sweden.

Early kidney differentiation is driven by local cell-cell interactions. The metanephrogenic mesenchyme stimulates the epithelial ureter bud to grow and branch, whereas the ureter bud stimulates the mesenchyme to convert into a new epithelium. These interactions may be dependent on local growth factors and their receptors. We studied the expression of receptors for nerve growth factors during kidney development. Expression of the low- and high-affinity receptors was cell-type specific. The low-affinity NGF receptor was found in the uninduced mesenchyme at early developmental stages, but in the glomerular podocytes at later developmental stages. In contrast, the high-affinity trkB receptor was found in the cortical mesenchyme cells that will differentiate into stroma. The trkC receptor was found only weakly expressed and in a few parts of the collecting ducts. The role of these receptors and c-ros, a receptor-type kinase expressed on the tip of the ureter bud, was studied by modified antisense oligonucleotides. However, we found that both sense, antisense and nonsense phosphorothioate oligonucleotides inhibited mouse and rat embryonic kidney development in vitro. The oligonucleotides appeared to be toxic for rodent embryonic kidneys in the experimental conditions that we used. Moreover, oligonucleotides did not penetrate well into the epithelial sheets in the organ cultures. We conclude that studies with phosphorothioate antisense oligonucleotides in organ cultures of embryonic kidneys should be interpreted with caution. Our current data do not allow us to not assign a function for the low- or high-affinity NGF receptors or c-ros in kidney development.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Sims-Lucas, L. Cullen-McEwen, V. P. Eswarakumar, D. Hains, K. Kish, B. Becknell, J. Zhang, J. F. Bertram, F. Wang, and C. M. Bates
Deletion of Frs2{alpha} from the ureteric epithelium causes renal hypoplasia
Am J Physiol Renal Physiol, November 1, 2009; 297(5): F1208 - F1219.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. A. Davies, M. Ladomery, P. Hohenstein, L. Michael, A. Shafe, L. Spraggon, and N. Hastie
Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation
Hum. Mol. Genet., January 15, 2004; 13(2): 235 - 246.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
A. Eggert, M. A. Grotzer, N. Ikegaki, H. Zhao, A. Cnaan, G. M. Brodeur, and A. E. Evans
Expression of the Neurotrophin Receptor TrkB Is Associated With Unfavorable Outcome in Wilms' Tumor
J. Clin. Oncol., February 1, 2001; 19(3): 689 - 696.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Li, R. O. Stuart, J. Qiao, A. Pavlova, K. T. Bush, M. Pohl, H. Sakurai, and S. K. Nigam
A role for Timeless in epithelial morphogenesis during kidney development
PNAS, August 29, 2000; 97(18): 10038 - 10043.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C Brakebusch, E Hirsch, A Potocnik, and R Fassler
Genetic analysis of beta1 integrin function: confirmed, new and revised roles for a crucial family of cell adhesion molecules
J. Cell Sci., January 12, 1997; 110(23): 2895 - 2904.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. Tazi, S. Le Bras, H. O. Lamghitnia, J. D. Vincent, P. Czernichow, and R. Scharfmann
Neurotrophin-3 Increases Intracellular Calcium in a Rat Insulin-secreting Cell Line through Its Action on a Functional TrkC Receptor
J. Biol. Chem., April 26, 1996; 271(17): 10154 - 10160.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M Falk, K Salmivirta, M Durbeej, E Larsson, M Ekblom, D Vestweber, and P Ekblom
Integrin alpha 6B beta 1 is involved in kidney tubulogenesis in vitro
J. Cell Sci., January 12, 1996; 109(12): 2801 - 2810.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993