|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 120, Issue 10 2811-2822, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
DE Emerling and AD Lander
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, 02139, USA.
In nervous system development, the growth cones of advancing axons are thought to navigate to their targets by recognizing cell-surface and extracellular matrix molecules that act as specific guidance cues. To identify and map cues that guide the growth of a particular axonal system, the thalamocortical afferents, an assay was devised to examine short-term interactions of dissociated embryonic thalamic cells with living, approximately 150 microns slices of developing mouse forebrain. Thalamic cells rapidly (< 3 hours) and efficiently attached to and extended neurites on pre- and postnatal slices, but a broad zone throughout the neocortex was generally non-permissive for both thalamic cell attachment and the ingrowth of neurites. This zone coincided with the cortical plate at early stages (embryonic day 15), but later became restricted, in rostral-to-caudal fashion, to cortical laminae 2/3. Thus, at each stage, thalamic cells in vitro avoided just that area that thalamic axons confront, but generally do not enter, in vivo. In addition, neurites that extended on some layers were found to be significantly oriented in directions that coincide with the pathways that thalamic axons follow in vivo. These results imply that local adhesive cues and signals that affect process outgrowth are distributed among developing cortical laminae in a manner that could underlie much of the temporal and spatial patterning of thalamocortical innervation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Poskanzer, L. A. Needleman, O. Bozdagi, and G. W. Huntley N-Cadherin Regulates Ingrowth and Laminar Targeting of Thalamocortical Axons J. Neurosci., March 15, 2003; 23(6): 2294 - 2305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Polleux and A. Ghosh The Slice Overlay Assay: A Versatile Tool to Study the Influence of Extracellular Signals on Neuronal Development Sci. Signal., June 11, 2002; 2002(136): pl9 - pl9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Becker and T. Becker Repellent Guidance of Regenerating Optic Axons by Chondroitin Sulfate Glycosaminoglycans in Zebrafish J. Neurosci., February 1, 2002; 22(3): 842 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Skaliora, R. Adams, and C. Blakemore Morphology and Growth Patterns of Developing Thalamocortical Axons J. Neurosci., May 15, 2000; 20(10): 3650 - 3662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Polleux, R. J. Giger, D. D. Ginty, A. L. Kolodkin, and A. Ghosh Patterning of Cortical Efferent Projections by Semaphorin-Neuropilin Interactions Science, December 4, 1998; 282(5395): 1904 - 1906. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Molnar, R. Adams, and C. Blakemore Mechanisms Underlying the Early Establishment of Thalamocortical Connections in the Rat J. Neurosci., August 1, 1998; 18(15): 5723 - 5745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Forster, C Kaltschmidt, J Deng, H Cremer, T Deller, and M Frotscher Lamina-specific cell adhesion on living slices of hippocampus Development, January 9, 1998; 125(17): 3399 - 3410. [Abstract] [PDF]
|
|
|
|
|
|
N. Yamamoto, S. Higashi, and K. Toyama Stop and Branch Behaviors of Geniculocortical Axons: A Time-Lapse Study in Organotypic Cocultures J. Neurosci., May 15, 1997; 17(10): 3653 - 3663. [Abstract] [Full Text] [PDF]
|
|
