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Development, Vol 120, Issue 10 3043-3049, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
C Schulz and D Tautz
Zoologisches Institut, Universitat Munchen, Germany.
The subdivision of the anterior-posterior axis in Drosophila is achieved by a cascade of spatially regulated transcription factors which form short-range gradients at the syncytial blastoderm stage. These factors are assumed to have concentration-dependent regulatory effects on their target genes. However, there is so far little direct in vivo evidence that a single factor can autonomously activate and repress a given target gene. We have analysed here the regulatory capabilities of the gap gene hunchback by creating an artificial gradient of hunchback in the early embryo. This was achieved by providing the maternally expressed mRNA of hunchback with the anterior localization signal of the bicoid RNA. The effects of this artificial hunchback gradient were then studied in different types of mutant background. We show that under these conditions hb is autonomously capable of activating the target gene Kruppel at low concentrations and repressing it at high concentrations. In addition, we show that the artificially created hunchback gradient can organize a large part of the segment pattern, although it is expressed at a different position and in a different shape than the wild-type gradient of hunchback.
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V. Schaeffer, F. Janody, C. Loss, C. Desplan, and E. A. Wimmer Bicoid functions without its TATA-binding protein-associated factor interaction domains PNAS, April 13, 1999; 96(8): 4461 - 4466. [Abstract] [Full Text] [PDF]
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X Wu, R Vakani, and S Small Two distinct mechanisms for differential positioning of gene expression borders involving the Drosophila gap protein giant Development, January 10, 1998; 125(19): 3765 - 3774. [Abstract] [PDF]
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C Wolff, R Schroder, C Schulz, D Tautz, and M Klingler Regulation of the Tribolium homologues of caudal and hunchback in Drosophila: evidence for maternal gradient systems in a short germ embryo Development, January 9, 1998; 125(18): 3645 - 3654. [Abstract] [PDF]
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C Wolff, R Sommer, R Schroder, G Glaser, and D Tautz Conserved and divergent expression aspects of the Drosophila segmentation gene hunchback in the short germ band embryo of the flour beetle Tribolium Development, January 12, 1995; 121(12): 4227 - 4236. [Abstract] [PDF]
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C Schulz and D Tautz Zygotic caudal regulation by hunchback and its role in abdominal segment formation of the Drosophila embryo Development, January 4, 1995; 121(4): 1023 - 1028. [Abstract] [PDF]
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