|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 120, Issue 11 3267-3274, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
J Helms, C Thaller and G Eichele
Department of Otorhinolaryngology, Baylor College of Medicine, Houston, Texas 77030.
Local application of all-trans-retinoic acid (RA) to the anterior margin of chick limb buds results in pattern duplications reminescent of those that develop after grafting cells from the zone of polarizing activity (ZPA). RA may act directly by conferring positional information to limb bud cells, or it may act indirectly by creating a polarizing region in the tissue distal to the RA source. Here we demonstrate that tissue distal to an RA-releasing bead acquires polarizing activity in a dose-dependent manner. Treatments with pharmacological (beads soaked in 330 micrograms/ml) and physiological (beads soaked in 10 micrograms/ml) doses of RA are equally capable of inducing digit pattern duplication. Additionally, both treatments induce sonic hedgehog (shh; also known as vertebrate hedgehog-1, vhh-1), a putative ZPA morphogen and Hoxd-11, a gene induced by the polarizing signal. However, tissue transplantation assays reveal that pharmacological, but not physiological, doses create a polarizing region. This differential response could be explained if physiological doses induced less shh than pharmacological doses. However, our in situ hybridization analyses demonstrate that both treatments result in similar amounts of mRNA encoding this candidate ZPA morphogen. We outline a model describing the apparently disparate effects of pharmacologic and physiological doses RA on limb bud tissue.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Ribes, I. Le Roux, M. Rhinn, B. Schuhbaur, and P. Dolle Early mouse caudal development relies on crosstalk between retinoic acid, Shh and Fgf signalling pathways Development, February 15, 2009; 136(4): 665 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ribes, Z. Wang, P. Dolle, and K. Niederreither Retinaldehyde dehydrogenase 2 (RALDH2)-mediated retinoic acid synthesis regulates early mouse embryonic forebrain development by controlling FGF and sonic hedgehog signaling Development, January 15, 2006; 133(2): 351 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Krebs, C. M. Schreiner, W. J. Scott Jr, S. M. Bell, D. J. Robbins, J. A. Goetz, H. Alt, N. Hawes, E. Wolf, and J. Favor Replicated anterior zeugopod (raz): a polydactylous mouse mutant with lowered Shh signaling in the limb bud Development, December 15, 2003; 130(24): 6037 - 6047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Ros, R. D. Dahn, M. Fernandez-Teran, K. Rashka, N. C. Caruccio, S. M. Hasso, J. J. Bitgood, J. J. Lancman, and J. F. Fallon The chick oligozeugodactyly (ozd) mutant lacks sonic hedgehog function in the limb Development, February 1, 2003; 130(3): 527 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Purton, I. D. Bernstein, and S. J. Collins All-trans retinoic acid enhances the long-term repopulating activity of cultured hematopoietic stem cells Blood, January 15, 2000; 95(2): 470 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Mercader, E Leonardo, M. Piedra, C Martinez-A, M. Ros, and M Torres Opposing RA and FGF signals control proximodistal vertebrate limb development through regulation of Meis genes Development, January 9, 2000; 127(18): 3961 - 3970. [Abstract] [PDF]
|
|
|
|
|
|
L. E. Purton, I. D. Bernstein, and S. J. Collins All-Trans Retinoic Acid Delays the Differentiation of Primitive Hematopoietic Precursors (lin-c-kit+Sca-1+) While Enhancing the Terminal Maturation of Committed Granulocyte/Monocyte Progenitors Blood, July 15, 1999; 94(2): 483 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Warburton, J. Zhao, M. A. Berberich, and M. Bernfield Molecular embryology of the lung: then, now, and in the future Am J Physiol Lung Cell Mol Physiol, May 1, 1999; 276(5): L697 - L704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Franco, A. Paganelli, S. Lopez, and A. Carrasco Functional association of retinoic acid and hedgehog signaling in Xenopus primary neurogenesis Development, January 10, 1999; 126(19): 4257 - 4265. [Abstract] [PDF]
|
|
|
|
 |
|
 S. K. Kim and D. A. Melton Pancreas development is promoted by cyclopamine, a Hedgehog signaling inhibitor PNAS, October 27, 1998; 95(22): 13036 - 13041. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Levine, H. Roelink, J. Turner, and T. A. Reh Sonic Hedgehog Promotes Rod Photoreceptor Differentiation in Mammalian Retinal Cells In Vitro J. Neurosci., August 15, 1997; 17(16): 6277 - 6288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lu, J. Revelli, L Goering, C Thaller, and G Eichele Retinoid signaling is required for the establishment of a ZPA and for the expression of Hoxb-8, a mediator of ZPA formation Development, January 5, 1997; 124(9): 1643 - 1651. [Abstract] [PDF]
|
|
|
|
|
|
J. Chin and Z Werb Matrix metalloproteinases regulate morphogenesis, migration and remodeling of epithelium, tongue skeletal muscle and cartilage in the mandibular arch Development, January 4, 1997; 124(8): 1519 - 1530. [Abstract] [PDF]
|
|
|
|
|
|
J. Helms, C. Kim, G Eichele, and C Thaller Retinoic acid signaling is required during early chick limb development Development, January 5, 1996; 122(5): 1385 - 1394. [Abstract] [PDF]
|
|
|
|
|
|
T Ogura, I. Alvarez, A Vogel, C Rodriguez, R. Evans, and J. Izpisua Belmonte Evidence that Shh cooperates with a retinoic acid inducible co-factor to establish ZPA-like activity Development, January 2, 1996; 122(2): 537 - 542. [Abstract] [PDF]
|
|
