spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, X.
Right arrow Articles by Sarras, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, X.
Right arrow Articles by Sarras, M. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 120, Issue 2 425-432, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

Cell-extracellular matrix interactions under in vivo conditions during interstitial cell migration in Hydra vulgaris

X Zhang and MP Sarras
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City 66160-7400.

Interstitial cell (I-cell) migration in hydra is essential for establishment of the regional cell differentiation pattern in the organism. All previous in vivo studies have indicated that cell migration in hydra is a result of cell-cell interactions and chemotaxic gradients. Recently, in vitro cell adhesion studies indicated that isolated nematocytes could bind to substrata coated with isolated hydra mesoglea, fibronectin and type IV collagen. Under these conditions, nematocytes could be observed to migrate on some of these extracellular matrix components. By modifying previously described hydra grafting techniques, two procedures were developed to test specifically the role of extracellular matrix components during in vivo I-cell migration in hydra. In one approach, the extracellular matrix structure of the apical half of the hydra graft was perturbed using beta-aminopropionitrile and beta-xyloside. In the second approach, grafts were treated with fibronectin, RGDS synthetic peptide and antibody to fibronectin after grafting was performed. In both cases, I-cell migration from the basal half to the apical half of the grafts was quantitatively analyzed. Statistical analysis indicated that beta-aminopropionitrile, fibronectin, RGDS synthetic peptide and antibody to fibronectin all were inhibitory to I-cell migration as compared to their respective controls. beta-xyloside treatment had no effect on interstitial cell migration. These results indicate the potential importance of cell-extracellular matrix interactions during in vivo I-cell migration in hydra.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J.-B. Michel
Anoikis in the Cardiovascular System: Known and Unknown Extracellular Mediators
Arterioscler Thromb Vasc Biol, December 1, 2003; 23(12): 2146 - 2154.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. Shimizu, X. Zhang, J. Zhang, A. Leontovich, K. Fei, L. Yan, and M. P. Sarras Jr.
Epithelial morphogenesis in hydra requires de novo expression of extracellular matrix components and matrix metalloproteinases
Development, March 5, 2003; 129(6): 1521 - 1532.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R Deutzmann, S Fowler, X Zhang, K Boone, S Dexter, R. Boot-Handford, R Rachel, and M. Sarras
Molecular, biochemical and functional analysis of a novel and developmentally important fibrillar collagen (Hcol-I) in hydra
Development, January 11, 2000; 127(21): 4669 - 4680.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. L. Brower, S. M. Brower, D. C. Hayward, and E. E. Ball
Molecular evolution of integrins: Genes encoding integrin beta  subunits from a coral and a sponge
PNAS, August 19, 1997; 94(17): 9182 - 9187.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Takahashi, Y. Muneoka, J. Lohmann, M. S. L. de Haro, G. Solleder, T. C. G. Bosch, C. N. David, H. R. Bode, O. Koizumi, H. Shimizu, et al.
Systematic isolation of peptide signal molecules regulating development in hydra: LWamide and PW families
PNAS, February 18, 1997; 94(4): 1241 - 1246.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L Yan, G. Pollock, H Nagase, and M. Sarras
A 25.7 x 10(3) M(r) hydra metalloproteinase (HMP1), a member of the astacin family, localizes to the extracellular matrix of Hydra vulgaris in a head-specific manner and has a developmental function
Development, January 6, 1995; 121(6): 1591 - 1602.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Fowler, S. Jose, X. Zhang, R. Deutzmann, M. P. Sarras Jr., and R. P. Boot-Handford
Characterization of Hydra Type IV Collagen. TYPE IV COLLAGEN IS ESSENTIAL FOR HEAD REGENERATION AND ITS EXPRESSION IS UP-REGULATED UPON EXPOSURE TO GLUCOSE
J. Biol. Chem., December 8, 2000; 275(50): 39589 - 39599.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1994