Development, Vol 120, Issue 3 673-681, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

bundle sheath defective, a mutation that disrupts cellular differentiation in maize leaves

J. A. Langdale and C. A. Kidner

Post-primordial differentiation events in developing maize leaves produce two photosynthetic cell types (bundle sheath and mesophyll) that are morphologically and biochemically distinct. We have isolated a mutation that disrupts the differentiation of one of these cell types in light-grown leaves. bundle sheath defective 1-mutable 1 (bsd1-m1) is an unstable allele that was induced by transposon mutagenesis. In the bundle sheath cells of bsd1-m1 leaves, chloroplasts differentiate aberrantly and C4 photosynthetic enzymes are absent. The development of mesophyll cells is unaffected. In dark-grown bsd1-m1 seedlings, morphological differentiation of etioplasts is only disrupted in bundle sheath cells but photosynthetic enzyme accumulation patterns are altered in both cell types. These data suggest that, during normal development, the Bsd1 gene directs the morphological differentiation of chloroplasts in a light-independent and bundle sheath cell-specific fashion. In contrast, Bsd1 gene action on photosynthetic gene expression patterns is cell-type independent in the dark (C3 state) but bundle sheath cell-specific in the light (C4 state). Current models hypothesize that C4 photosynthetic differentiation is achieved through a light-induced interaction between bundle sheath and mesophyll cells (J. A. Langdale and T. Nelson (1991) Trends in Genetics 7, 191-196). Based on the data shown in this paper, we propose that induction of the C4 state restricts Bsd1 gene action to bundle sheath cells.

This article has been cited by other articles:

				D. M. Braun, Y. Ma, N. Inada, M. G. Muszynski, and R. F. Baker tie-dyed1 Regulates Carbohydrate Accumulation in Maize Leaves Plant Physiology, December 1, 2006; 142(4): 1511 - 1522. [Abstract] [Full Text] [PDF]

				W. Majeran, Y. Cai, Q. Sun, and K. J. van Wijk Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics PLANT CELL, November 1, 2005; 17(11): 3111 - 3140. [Abstract] [Full Text] [PDF]

				Y. Yasumura, E. C. Moylan, and J. A. Langdale A Conserved Transcription Factor Mediates Nuclear Control of Organelle Biogenesis in Anciently Diverged Land Plants PLANT CELL, July 1, 2005; 17(7): 1894 - 1907. [Abstract] [Full Text] [PDF]

				L. Cribb, L. N. Hall, and J. A. Langdale Four Mutant Alleles Elucidate the Role of the G2 Protein in the Development of C4 and C3 Photosynthesizing Maize Tissues Genetics, October 1, 2001; 159(2): 787 - 797. [Abstract] [Full Text] [PDF]

				L. Rossini, L. Cribb, D. J. Martin, and J. A. Langdale The Maize Golden2 Gene Defines a Novel Class of Transcriptional Regulators in Plants PLANT CELL, May 1, 2001; 13(5): 1231 - 1244. [Abstract] [Full Text]

				G. Shu, V. Pontieri, N. G. Dengler, and L. J. Mets Light Induction of Cell Type Differentiation and Cell-Type-Specific Gene Expression in Cotyledons of a C4 Plant, Flaveria trinervia Plant Physiology, November 1, 1999; 121(3): 731 - 741. [Abstract] [Full Text]

				T. P. Brutnell, R. J. H. Sawers, A. Mant, and J. A. Langdale BUNDLE SHEATH DEFECTIVE2, a Novel Protein Required for Post-Translational Regulation of the rbcL Gene of Maize PLANT CELL, May 1, 1999; 11(5): 849 - 864. [Abstract] [Full Text]

				L. N. Hall, L. Rossini, L. Cribb, and J. A. Langdale GOLDEN 2: A Novel Transcriptional Regulator of Cellular Differentiation in the Maize Leaf PLANT CELL, June 1, 1998; 10(6): 925 - 936. [Abstract] [Full Text]

				O. Ueno Induction of Kranz Anatomy and C4-like Biochemical Characteristics in a Submerged Amphibious Plant by Abscisic Acid PLANT CELL, April 1, 1998; 10(4): 571 - 584. [Abstract] [Full Text]

				E. Kinsman and K. Pyke Bundle sheath cells and cell-specific plastid development in Arabidopsis leaves Development, January 5, 1998; 125(10): 1815 - 1822. [Abstract] [PDF]