spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rotello, R. J.
Right arrow Articles by Yuan, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rotello, R. J.
Right arrow Articles by Yuan, J.

Development, Vol 120, Issue 6 1421-1431, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

Anti-apogens and anti-engulfens: monoclonal antibodies reveal specific antigens on apoptotic and engulfment cells during chicken embryonic development

RJ Rotello, PA Fernandez and J Yuan
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129.

We have isolated a group of monoclonal antibodies that specifically recognize either apoptotic or engulfment cells in the interdigit areas of chicken hind limb foot plates, and throughout the embryo. Ten of these antibodies (anti-apogens) detect epitopes on dying cells that colocalize to areas of programmed cell death, characterized by the presence of apoptotic cells and bodies with typical cellular and nuclear morphology. Our results indicate that cells destined to die, or that are in the process of dying, express specific antigens that are not detectable in or on the surface of living cells. The detection of these apoptotic cell antigens in other areas of programmed cell death throughout the chick embryo indicates that different cell types, which form specific tissues and organs, may utilize similar cell death mechanisms. Six of the monoclonal antibodies (antiengulfens) define a class of engulfment cells which contain various numbers of apoptotic cells and/or apoptotic bodies in areas of programmed cell death. The immunostaining pattern of the anti-engulfen R15F is similar to that of an antibody against a common leukocyte antigen, suggesting the participation of cells from the immune system in the removal of apoptotic cell debris. These novel monoclonal antibody markers for apoptotic and engulfment cells will provide new tools to assist the further understanding of developmental programmed cell death in vertebrates.


This article has been cited by other articles:


Home page
 DevelopmentHome page
W Wood, M Turmaine, R Weber, V Camp, R. Maki, S. McKercher, and P Martin
Mesenchymal cells engulf and clear apoptotic footplate cells in macrophageless PU.1 null mouse embryos
Development, January 12, 2000; 127(24): 5245 - 5252.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
U. E. Knies, H. A. Behrensdorf, C. A. Mitchell, U. Deutsch, W. Risau, H. C. A. Drexler, and M. Clauss
Regulation of endothelial monocyte-activating polypeptide II release by apoptosis
PNAS, October 13, 1998; 95(21): 12322 - 12327.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1994