|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 120, Issue 6 1685-1693, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
EJ Williams, J Furness, FS Walsh and P Doherty
Department of Experimental Pathology, UMDS, Guy's Hospital, London Bridge, London, UK.
Cerebellar neurons, cultured on monolayers of 3T3 fibroblasts or on a polylysine/laminin-coated substratum, responded to recombinant basic FGF by extending longer neurites. The response was biphasic reaching a maximum at 5 ng/ml FGF, but desensitising at 100-200 ng/ml FGF. The response to FGF could be inhibited by a tyrosine kinase inhibitor (the erbstatin analogue), by a diacylglycerol lipase inhibitor (RHC-80267) and by a combination of N- and L-type calcium channel antagonists or other agents that negate the effects of calcium influx into neurons. The response to FGF could be fully mimicked by arachidonic acid added directly to the cultures, or generated via activation of phospholipase A2 with melittin. The response to melittin, but not to FGF or arachidonic acid, was inhibited by 4-bromophenacyl bromide, a phospholipase A2 inhibitor. The response to arachidonic acid was also biphasic and high concentrations of this agent could cross-desensitise the FGF response and vice versa. The response to arachidonic acid could be fully inhibited by the agents that block or negate the effects of calcium influx into neurons, but was not inhibited by the tyrosine kinase or diacylglycerol lipase inhibitors. These data suggest that FGF stimulates neurite outgrowth by activating a cascade that involves activation of phospholipase C gamma to produce diacylglycerol, conversion of diacylglycerol to arachidonic acid by diacylglycerol lipase and the activation of voltage-gated calcium channels by arachidonic acid.
This article has been cited by other articles:
|
|
 |
|
  M. P. Fogarty, B. A. Emmenegger, L. L. Grasfeder, T. G. Oliver, and R. J. Wechsler-Reya Fibroblast growth factor blocks Sonic hedgehog signaling in neuronal precursors and tumor cells PNAS, February 20, 2007; 104(8): 2973 - 2978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mitsunaga, T. Mikami, S. Mizumoto, J. Fukuda, and K. Sugahara Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains in the Development of Cerebellum: SPATIOTEMPORAL REGULATION OF THE EXPRESSION OF CRITICAL DISULFATED DISACCHARIDES BY SPECIFIC SULFOTRANSFERASES J. Biol. Chem., July 14, 2006; 281(28): 18942 - 18952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Marszalek, C. Kitidis, C. C. DiRusso, and H. F. Lodish Long-chain Acyl-CoA Synthetase 6 Preferentially Promotes DHA Metabolism J. Biol. Chem., March 18, 2005; 280(11): 10817 - 10826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dityatev, G. Dityateva, V. Sytnyk, M. Delling, N. Toni, I. Nikonenko, D. Muller, and M. Schachner Polysialylated Neural Cell Adhesion Molecule Promotes Remodeling and Formation of Hippocampal Synapses J. Neurosci., October 20, 2004; 24(42): 9372 - 9382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Marszalek, C. Kitidis, A. Dararutana, and H. F. Lodish Acyl-CoA Synthetase 2 Overexpression Enhances Fatty Acid Internalization and Neurite Outgrowth J. Biol. Chem., June 4, 2004; 279(23): 23882 - 23891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-J. Williams, F. S. Walsh, and P. Doherty The FGF receptor uses the endocannabinoid signaling system to couple to an axonal growth response J. Cell Biol., February 18, 2003; 160(4): 481 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Williams, E.-J. Williams, and P. Doherty Dimeric Versions of Two Short N-cadherin Binding Motifs (HAVDI and INPISG) Function as N-cadherin Agonists J. Biol. Chem., February 1, 2002; 277(6): 4361 - 4367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-J. Williams, G. Williams, F. V. Howell, S. D. Skaper, F. S. Walsh, and P. Doherty Identification of an N-cadherin Motif That Can Interact with the Fibroblast Growth Factor Receptor and Is Required for Axonal Growth J. Biol. Chem., November 16, 2001; 276(47): 43879 - 43886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. ROSENTHAL, H. THIEME, and O. STRAUSS Fibroblast growth factor receptor 2 (FGFR2) in brain neurons and retinal pigment epithelial cells act via stimulation of neuroendocrine L-type channels (Cav1.3) FASEB J, April 1, 2001; 15(6): 970 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Schaefer, H. Kamiguchi, E. V. Wong, C. M. Beach, G. Landreth, and V. Lemmon Activation of the MAPK Signal Cascade by the Neural Cell Adhesion Molecule L1 Requires L1 Internalization J. Biol. Chem., December 31, 1999; 274(53): 37965 - 37973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Bearer, A. R. Swick, M. A. O'Riordan, and G. Cheng Ethanol Inhibits L1-mediated Neurite Outgrowth in Postnatal Rat Cerebellar Granule Cells J. Biol. Chem., May 7, 1999; 274(19): 13264 - 13270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. F. Meiri, J. L. Saffell, F. S. Walsh, and P. Doherty Neurite Outgrowth Stimulated by Neural Cell Adhesion Molecules Requires Growth-Associated Protein-43 (GAP-43) Function and Is Associated with GAP-43 Phosphorylation in Growth Cones J. Neurosci., December 15, 1998; 18(24): 10429 - 10437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Aplin, A. Howe, S. K. Alahari, and R. L. Juliano Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins Pharmacol. Rev., June 1, 1998; 50(2): 197 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Yip, X. Zhao, A. M.P. Montgomery, and C.-H. Siu The Arg-Gly-Asp Motif in the Cell Adhesion Molecule L1 Promotes Neurite Outgrowth via Interaction with the alpha vbeta 3 Integrin Mol. Biol. Cell, February 1, 1998; 9(2): 277 - 290. [Abstract] [Full Text]
|
|
|
|
|
|
M. Zhu, C. H. Gelband, J. M. Moore, P. Posner, and C. Sumners Angiotensin II Type 2 Receptor Stimulation of Neuronal Delayed-Rectifier Potassium Current Involves Phospholipase A2 and Arachidonic Acid J. Neurosci., January 15, 1998; 18(2): 679 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Charlton, W. A. Mohler, G. L. Radice, R. O. Hynes, and H. M. Blau Fusion Competence of Myoblasts Rendered Genetically Null for N-Cadherin in Culture J. Cell Biol., July 28, 1997; 138(2): 331 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. D. Garver, Q. Ren, S. Tuvia, and V. Bennett Tyrosine Phosphorylation at a Site Highly Conserved in the L1 Family of Cell Adhesion Molecules Abolishes Ankyrin Binding and Increases Lateral Mobility of Neurofascin J. Cell Biol., May 5, 1997; 137(3): 703 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bikfalvi, S. Klein, G. Pintucci, and D. B. Rifkin Biological Roles of Fibroblast Growth Factor-2 Endocr. Rev., February 1, 1997; 18(1): 26 - 45. [Abstract] [Full Text]
|
|
|
|
|
|
X. Zhao and C.-H. Siu Colocalization of the Homophilic Binding Site and the Neuritogenic Activity of the Cell Adhesion Molecule L1 to Its Second Ig-like Domain J. Biol. Chem., December 8, 1995; 270(49): 29413 - 29421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Williams, B Mittal, F. Walsh, and P Doherty FGF inhibits neurite outgrowth over monolayers of astrocytes and fibroblasts expressing transfected cell adhesion molecules J. Cell Sci., January 11, 1995; 108(11): 3523 - 3530. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Lamb and R. Harland Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anterior-posterior neural pattern Development, January 11, 1995; 121(11): 3627 - 3636. [Abstract] [PDF]
|
|
|
|
|
|
A. Stoker, B Gehrig, F Haj, and B. Bay Axonal localisation of the CAM-like tyrosine phosphatase CRYP alpha: a signalling molecule of embryonic growth cones Development, January 6, 1995; 121(6): 1833 - 1844. [Abstract] [PDF]
|
|
