|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 120, Issue 7 1851-1859, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
Z Xu, GS Kopf and RM Schultz
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104.
Sperm-induced activation of mammalian eggs is associated with a transient increase in the concentration of intracellular Ca2+. The role of inositol 1,4,5-trisphosphate (IP3)-mediated release of Ca2+ from intracellular stores during mouse egg activation was examined in the present study by determining the effects of microinjected monoclonal antibody (mAb) 18A10, which binds to the IP3 receptor and inhibits IP3-induced Ca2+ release, on endpoints of egg activation following insemination. The antibody inhibited in a concentration-dependent manner the ZP2 to ZP2f conversion that is involved in the zona pellucida block to polyspermy, as well as the ZP2 to ZP2f conversion promoted by microinjected IP3 in non-inseminated eggs. As anticipated, inseminated eggs that had been microinjected with the antibody were polyspermic. In addition, the antibody inhibited the fertilization-associated decrease in H1 kinase activity and pronucleus formation, and the concentration dependence for inhibition of these events was similar to that observed for inhibiting the ZP2 to ZP2f conversion. Last, the antibody inhibited the fertilization-induced recruitment of maternal mRNAs and post-translational modifications of proteins. In each case, eggs microinjected with the mAb 4C11, which also binds to the IP3 receptor but does not inhibit IP3-induced Ca2+ release, had no inhibitory effect on fertilization and egg activation. Results of these studies suggest that IP3-mediated Ca2+ release is essential for both early and late events of mouse egg activation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Cui, K. L. Sackton, V. L. Horner, K. E. Kumar, and M. F. Wolfner Wispy, the Drosophila Homolog of GLD-2, Is Required During Oogenesis and Egg Activation Genetics, April 1, 2008; 178(4): 2017 - 2029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-Y. Yoon and R. A Fissore Release of phospholipase C {zeta}and [Ca2+]i oscillation-inducing activity during mammalian fertilization Reproduction, November 1, 2007; 134(5): 695 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Malcuit, J. G. Knott, C. He, T. Wainwright, J. B. Parys, J. M. Robl, and R. A. Fissore Fertilization and Inositol 1,4,5-Trisphosphate (IP3)-Induced Calcium Release in Type-1 Inositol 1,4,5-Trisphosphate Receptor Down-Regulated Bovine Eggs Biol Reprod, July 1, 2005; 73(1): 2 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Knott, M. Kurokawa, R. A. Fissore, R. M. Schultz, and C. J. Williams Transgenic RNA Interference Reveals Role for Mouse Sperm Phospholipase C{zeta} in Triggering Ca2+ Oscillations During Fertilization Biol Reprod, April 1, 2005; 72(4): 992 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Laporte, A. Hui, and I. Laher Pharmacological Modulation of Sarcoplasmic Reticulum Function in Smooth Muscle Pharmacol. Rev., December 1, 2004; 56(4): 439 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Halet, R. Tunwell, S. J. Parkinson, and J. Carroll Conventional PKCs regulate the temporal pattern of Ca2+ oscillations at fertilization in mouse eggs J. Cell Biol., March 29, 2004; 164(7): 1033 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Tosti and R. Boni Electrical events during gamete maturation and fertilization in animals and humans Hum. Reprod. Update, January 1, 2004; 10(1): 53 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.T. Goud, A.P. Goud, L. Leybaert, P.V. Oostveldt, K. Mikoshiba, M.P. Diamond, and M. Dhont Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP3 are blocked by a specific antibody to the type I receptor Mol. Hum. Reprod., October 1, 2002; 8(10): 912 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Halet, R. Tunwell, T. Balla, K. Swann, and J. Carroll The dynamics of plasma membrane PtdIns(4,5)P2 at fertilization of mouse eggs J. Cell Sci., May 15, 2002; 115(10): 2139 - 2149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Knott, K. Poothapillai, H. Wu, C. L. He, R. A. Fissore, and J. M. Robl Porcine Sperm Factor Supports Activation and Development of Bovine Nuclear Transfer Embryos Biol Reprod, April 1, 2002; 66(4): 1095 - 1103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fissore, F. J. Longo, E. Anderson, J. B. Parys, and T. Ducibella Differential Distribution of Inositol Trisphosphate Receptor Isoforms in Mouse Oocytes Biol Reprod, January 1, 1999; 60(1): 49 - 57. [Abstract] [Full Text]
|
|
|
|
|
|
A. L. Abbott, Z. Xu, G. S. Kopf, T. Ducibella, and R. M. Schultz In Vitro Culture Retards Spontaneous Activation of Cell Cycle Progression and Cortical Granule Exocytosis That Normally Occur in In Vivo UnfertilizedMouse Eggs Biol Reprod, December 1, 1998; 59(6): 1515 - 1521. [Abstract] [Full Text]
|
|
|
|
|
|
C. Sette, A. Bevilacqua, R. Geremia, and P. Rossi Involvement of Phospholipase C{gamma}1 in Mouse Egg Activation Induced by a Truncated Form of the C-kit Tyrosine Kinase Present in Spermatozoa J. Cell Biol., August 24, 1998; 142(4): 1063 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Stachecki and D. Armant Transient release of calcium from inositol 1,4,5-trisphosphate-specific stores regulates mouse preimplantation development Development, January 8, 1996; 122(8): 2485 - 2496. [Abstract] [PDF]
|
|
|
|
|
|
J Moos, G. Kopf, and R. Schultz Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities J. Cell Sci., January 4, 1996; 109(4): 739 - 748. [Abstract] [PDF]
|
|
|
|
 |
|
 A Ghosh and M. Greenberg Calcium signaling in neurons: molecular mechanisms and cellular consequences Science, April 14, 1995; 268(5208): 239 - 247. [Abstract] [PDF]
|
|
|
|
|
|
T Ayabe, G. Kopf, and R. Schultz Regulation of mouse egg activation: presence of ryanodine receptors and effects of microinjected ryanodine and cyclic ADP ribose on uninseminated and inseminated eggs Development, January 7, 1995; 121(7): 2233 - 2244. [Abstract] [PDF]
|
|
|
|
|
|
G. Moore, T Ayabe, P. Visconti, R. Schultz, and G. Kopf Roles of heterotrimeric and monomeric G proteins in sperm-induced activation of mouse eggs Development, January 11, 1994; 120(11): 3313 - 3323. [Abstract] [PDF]
|
|
